The transcriptional repressor EUO regulates both subsets of Chlamydia late genes

Molecular Microbiology

Volumn 94, Issue 4, 2014, Pages 888-897

  Rosario, Christopher J ^a   Hanson, Brett R ^a   Tan, Ming ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EUO PROTEIN; RNA POLYMERASE; SIGMA 66 RNA POLYMERASE; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; BACTERIAL DNA; BACTERIAL PROTEIN; FLIA PROTEIN, BACTERIA; PROTEIN BINDING; REPRESSOR PROTEIN; SIGMA 66 FACTOR, CHLAMYDIA; SIGMA FACTOR;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BINDING AFFINITY; BINDING SITE; CHLAMYDIA; CONTROLLED STUDY; GENE EXPRESSION; GENE EXPRESSION REGULATION; IMMUNOPRECIPITATION; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN FUNCTION; PROTEIN LOCALIZATION; TRANSCRIPTION TERMINATION; CHLAMYDIA TRACHOMATIS; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; OPERATOR GENE;

BACTERIA (MICROORGANISMS); CHLAMYDIA; EUKARYOTA;

BACTERIAL PROTEINS; CHLAMYDIA TRACHOMATIS; DNA MUTATIONAL ANALYSIS; DNA, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; IMMUNOPRECIPITATION; OPERATOR REGIONS, GENETIC; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; REPRESSOR PROTEINS; SIGMA FACTOR; TRANSCRIPTION, GENETIC;

EID: 84930982118     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/mmi.12804     Document Type: Article

References (28)

1. Abdelrahman, Y.M., and Belland, R.J. (2005) The chlamydial developmental cycle. FEMS Microbiol Rev 29: 949-959.
2. Barry, C., Hayes, S., and Hackstadt, T. (1992) Nucleoid condensation in Escherichia coli that express a chlamydial histone homolog. Science 256: 377-379.
3. Belland, R.J., Zhong, G., Crane, D.D., Hogan, D., Sturdevant, D., Sharma, J., etal. (2003) Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis. Proc Natl Acad Sci USA 100: 8478-8483.
4. Betts-Hampikian, H.J., and Fields, K.A. (2010) The chlamydial type III secretion mechanism: revealing cracks in a tough nut. Front Microbiol 1: 114. doi: 10.3389/fmicb.2010.00114
5. Brickman, T.J., Barry, C.E., 3rd, and Hackstadt, T. (1993) Molecular cloning and expression of hctB encoding a strain-variant chlamydial histone-like protein with DNA-binding activity. J Bacteriol 175: 4274-4281.
6. Case, E.D., Peterson, E.M., and Tan, M. (2010) Promoters for Chlamydia type III secretion genes show a differential response to DNA supercoiling that correlates with temporal expression pattern. J Bacteriol 192: 2569-2574.
7. Clarke, I.N., Ward, M.E., and Lambden, P.R. (1988) Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis. Gene 71: 307-314.
8. Douglas, A.L., and Hatch, T.P. (2000) Expression of the transcripts of the sigma factors and putative sigma factor regulators of Chlamydia trachomatis L2. Gene 247: 209-214.
9. Kundu, T., Kusano, S., and Ishihama, A. (1997) Promoter selectivity of Escherichia coli RNA polymerase sigmaF holoenzyme involved in transcription of flagellar and chemotaxis genes. J Bacteriol 179: 4264-4269.
10. Lanzer, M., and Bujard, H. (1988) Promoters largely determine the efficiency of repressor action. Proc Natl Acad Sci USA 85: 8973-8977.
11. Liu, X., Afrane, M., Clemmer, D.E., Zhong, G., and Nelson, D.E. (2010) Identification of Chlamydia trachomatis outer membrane complex proteins by differential proteomics. J Bacteriol 192: 2852-2860.
12. Mukhopadhyay, S., Good, D., Miller, R.D., Graham, J.E., Mathews, S.A., Timms, P., and Summersgill, J.T. (2006) Identification of Chlamydia pneumoniae proteins in the transition from reticulate to elementary body formation. Mol Cell Proteomics 5: 2311-2318.
13. Nicholson, T.L., Olinger, L., Chong, K., Schoolnik, G., and Stephens, R.S. (2003) Global stage-specific gene regulation during the developmental cycle of Chlamydia trachomatis. J Bacteriol 185: 3179-3189.
14. Payankaulam, S., Li, L.M., and Arnosti, D.N. (2010) Transcriptional repression: conserved and evolved features. Curr Biol 20: R764-R771.
15. Read, M. (1996) Electrophoretic mobility shift assay (EMSA). In Gene Transcription: DNA Binding Proteins. Docherty, K. (ed.). Chichester, West Sussex, UK: John Wiley & Sons, pp. 6-11.
16. Rojo, F. (2001) Mechanisms of transcriptional repression. Curr Opin Microbiol 4: 145-151.
17. Rosario, C.J., and Tan, M. (2012) The early gene product EUO is a transcriptional repressor that selectively regulates promoters of Chlamydia late genes. Mol Microbiol 84: 1097-1107.
18. Rosario, C.J., Janes, B.K., and Bender, R.A. (2010) Genetic analysis of the nitrogen assimilation control protein from Klebsiella pneumoniae. J Bacteriol 192: 4834-4846.
19. Shaw, E.I., Dooley, C.A., Fischer, E.R., Scidmore, M.A., Fields, K.A., and Hackstadt, T. (2000) Three temporal classes of gene expression during the Chlamydia trachomatis developmental cycle. Mol Microbiol 37: 913-925.
20. Shen, L., Li, M., and Zhang, Y.X. (2004) Chlamydia trachomatis sigma28 recognizes the fliC promoter of Escherichia coli and responds to heat shock in chlamydiae. Microbiology 150: 205-215.
21. Tan, M. (2012) Temporal gene regulation during the chlamydial development cycle. In Intracellular Pathogens I: Chlamydiales. Tan, M., and Bavoil, P.M. (eds). Washington, DC: ASM Press, pp. 149-169.
22. Wichlan, D.G., and Hatch, T.P. (1993) Identification of an early-stage gene of Chlamydia psittaci 6BC. J Bacteriol 175: 2936-2942.
23. Wilson, A.C., and Tan, M. (2002) Functional analysis of the heat shock regulator HrcA of Chlamydia trachomatis. J Bacteriol 184: 6566-6571.
24. Yu, H.H., Di Russo, E.G., Rounds, M.A., and Tan, M. (2006a) Mutational analysis of the promoter recognized by Chlamydia and Escherichia coli sigma(28) RNA polymerase. J Bacteriol 188: 5524-5531.
25. Yu, H.H., Kibler, D., and Tan, M. (2006b) In silico prediction and functional validation of sigma28-regulated genes in Chlamydia and Escherichia coli. J Bacteriol 188: 8206-8212.
26. Yu, H.H.Y., and Tan, M. (2003) Sigma 28 RNA polymerase regulates hctB, a late developmental gene in Chlamydia. Mol Microbiol 50: 577-584.
27. Zhang, L., Douglas, A.L., and Hatch, T.P. (1998) Characterization of a Chlamydia psittaci DNA binding protein (EUO) synthesized during the early and middle phases of the developmental cycle. Infect Immun 66: 1167-1173.
28. Zhang, L., Howe, M.M., and Hatch, T.P. (2000) Characterization of in vitro DNA binding sites of the EUO protein of Chlamydia psittaci. Infect Immun 68: 1337-1349.