Direct identification of microorganisms from positive blood cultures using the lysis-filtration technique and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): A multicentre study

New Microbiologica

Volumn 38, Issue 2, 2015, Pages 245-250

  Farina, Claudio ^a   Arena, Fabio ^b   Casprini, Patrizia ^c   Cichero, Paola ^d   Clementi, Massimo ^d   Cosentino, Marina ^a   Degl'Innocenti, Roberto ^c   Giani, Tommaso ^b   Luzzaro, Francesco ^e   Mattei, Romano ^f   Mauri, Carola ^e   Nardone, Maria ^f   Rossolini, Gian Maria ^b,g,h,i   Ortega, Paula Andrea Serna ^a   Vailati, Francesca ^a  

^a OSPEDALI RIUNITI   (Italy)

^b UNIVERSITY OF SIENA   (Italy)

^c Local Health Authority N4   (Italy)

^d SAN RAFFAELE HOSPITAL   (Italy)

^e A MANZONI HOSPITAL   (Italy)

^f CAMPO DI MARTE HOSPITAL   (Italy)

^g UNIVERSITY HOSPITAL OF SIENA   (Italy)

^h UNIVERSITY OF FLORENCE   (Italy)

ⁱ CAREGGI UNIVERSITY HOSPITAL   (Italy)

Author keywords

Blood culture; Identification; Lysis filtration technique; MALDI TOF

Indexed keywords

BACTERIA (MICROORGANISMS); NEGIBACTERIA; POSIBACTERIA; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PNEUMONIAE;

BACTERIAL INFECTION; BACTERIUM; BACTERIUM IDENTIFICATION; BLOOD; CHEMISTRY; CLASSIFICATION; EVALUATION STUDY; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; MICROBIOLOGY; PROCEDURES;

BACTERIA; BACTERIAL INFECTIONS; BACTERIAL TYPING TECHNIQUES; BLOOD; HUMANS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84930971417     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Bizzini A., Greub G. (2010). Matrix-assisted laser desorption ionisation time-of-flight mass spectrom-etry, a revolution in clinical microbial identification. Clin. Microbiol. Infect. 16, 1614-1619.
2. Christner M., Rohde H., Wolters M., Sobattka I., Wegscheider K., Aepfelbacher M. (2010). Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spec-trometry fingerprinting. J. Clin. Microbiol. 48, 1584-1591.
3. Diekema D.J., Beekmann S.E., Chapin K.C., Morel K.A., Munson E.,. Doern G.V. (2003). Epidemiology and outcome of nosocomial and community-onset bloodstream infection. J. Clin. Microbiol. 41, 3655-3660.
4. Hrabák J., Chudácková E., Walková R. (2013). Matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry for detection of antibiotic resistance mechanisms: from research to routine diagnosis. Clin. Microbiol. Rev. 26, 103-114.
5. Idelevich Ea, Schüle I, Grünastel B., Wüllenweber J, Peters G, Becker K. (2014). Acceleration of antimicrobial susceptibility testing of positive blood cultures by inoculation of Vitek2 cards with briefly incubated solid medium cultures. J. Clin. Mi-crobiol. 52, 4058-4062.
6. La Scola B., Raoult D. (2009). Direct Identification of Bacteria in Positive Blood Culture Bottles by Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry. PLoS One. 4, e8041.
7. Loonen A.J., Jansz A.R., Stalpers J., Wolffs P.F., Van Der Brule A.J. (2012). An evaluation of three processing methods and the effect of reduced culture times for faster direct identification of pathogens from BacT/ALERT blood cultures by MALDI-TOF MS. Eur. J. Clin. Microbiol. Infect. Dis. 31, 1575-1583.
8. Mancini N., Carletti S., Ghidoli N., Cichero P., Burio-Ni R., Clementi M. (2010). The era of molecular and other non-culture-based methods in diagnosis of sepsis. Clin. Microbiol. Rev. 23, 235-251.
9. Patel R. (2013). Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Clinical Microbiology. Clin. Infect. Dis. 57, 564-572.
10. Rodríguez-Sánchez B, Sánchez-Carrillo C, Ruiz A, Marín M, Cercenado E, Rodríguez-Créixems M. (2014) Direct identification of pathogens from positive blood cultures using matrix-assisted laser desorption-ionization time-of-flight mass spec-trometry. Clin. Microbiol. Infect. 20, O421-O427.
11. Romero-Gómez M.P., Gómez-Gil R., Paño-Pardo J.R., Mingorance J. (2012). Identification and susceptibility testing of microorganism by direct inoculation from positive blood culture bottles by combining MALDI-TOF and Vitek-2 Compact is rapid and effective. J. Infect. 65, 513-520.
12. Seifert H. (2009). The clinical importance of microbiological findings in the diagnosis and management of bloodstream infections. Clin. Infect. Dis. 48 (Suppl. 4): S238-S245.
13. SØgaard M., NØrgaard M., SchØnheyder H.C. (2007). First notification of positive blood cultures and the high accuracy of the gram stain report. J. Clin. Microbiol. 45, 1113-1117.
14. Stevenson L.G., Drake S.K., Murray P.R. (2010). Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 48, 444-447.