An improved reverse dot hybridization for simple and rapid detection of adefovir dipivoxil-resistant hepatitis B virus

Genetics and Molecular Research

Volumn 11, Issue 1, 2012, Pages 53-60

  Hu, Y ^a   Zhang, W L ^a   Xie, S L ^a   Zhao, Y ^a   Hu, J L ^a   Cai, X F ^a   Lai, G Q ^a   Huang, A L ^a  

^a CHONGQING MEDICAL UNIVERSITY   (China)

Author keywords

Adefovir dipivoxil; Hepatitis B virus; Mutant; Reverse dot hybridization

Indexed keywords

ADEFOVIR DIPIVOXIL; OLIGONUCLEOTIDE;

ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DOT HYBRIDIZATION; GENE IDENTIFICATION; GENE MUTATION; GENE SEQUENCE; GENOTYPE; HEPATITIS B VIRUS; IMMOBILIZATION; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MOLECULAR CLONING; NONHUMAN; REVERSE DOT HYBRIDIZATION; SENSITIVITY ANALYSIS; STANDARDIZATION; VIRUS RESISTANCE; VIRUS STRAIN;

HEPATITIS B VIRUS;

EID: 84930482574     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: 10.4238/2012.January.9.6     Document Type: Article

References (18)

1. Aloman C and Wands JR (2003). Resistance of HBV to adefovir dipivoxil: a case for combination antiviral therapy? Hepatology 38: 1584-1587.
2. Chan V, Yam I, Chen FE and Chan TK (1999). A reverse dot-blot method for rapid detection of non-deletion alpha thalassaemia. Br. J. Haematol. 104: 513-515.
3. Dai CY, Chuang WL, Hsieh MY and Lee LP (2007). Adefovir dipivoxil treatment of lamivudine-resistant chronic hepatitis B. Antiviral Res. 75: 146-151.
4. Delaney WE (2007). Progress in the treatment of chronic hepatitis B: long-term experience with adefovir dipivoxil. J. Antimicrob. Chemother. 59: 827-832.
5. Fung SK, Chae HB, Fontana RJ, Conjeevaram H, et al. (2006). Virologic response and resistance to adefovir in patients with chronic hepatitis B. J. Hepatol. 44: 283-290.
6. Hadziyannis SJ, Tassopoulos NC, Heathcote EJ, Chang TT, et al. (2006). Long-term therapy with adefovir dipivoxil for HBeAg-negative chronic hepatitis B for up to 5 years. Gastroenterology 131: 1743-1751.
7. Lok AS, Zoulim F, Locarnini S, Bartholomeusz A, et al. (2007). Antiviral drug-resistant HBV: standardization of nomenclature and assays and recommendations for management. Hepatology 46: 254-265.
8. Lovicu M, Dessi V, Zappu A, De VS, et al. (2003). Efficient strategy for molecular diagnosis of Wilson disease in the sardinian population. Clin. Chem. 49: 496-498.
9. Luan J, Yuan J, Li X, Jin S, et al. (2009). Multiplex detection of 60 hepatitis B virus variants by maldi-tof mass spectrometry. Clin. Chem. 55: 1503-1509.
10. Moskovitz DN, Osiowy C, Giles E, Tomlinson G, et al. (2005). Response to long-term lamivudine treatment (up to 5 years) in patients with severe chronic hepatitis B, role of genotype and drug resistance. J. Viral. Hepat. 12: 398-404.
11. Ohishi W, Shirakawa H, Kawakami Y, Kimura S, et al. (2004). Identification of rare polymerase variants of hepatitis B virus using a two-stage PCR with peptide nucleic acid clamping. J. Med. Virol. 72: 558-565.
12. Osiowy C, Villeneuve JP, Heathcote EJ, Giles E, et al. (2006). Detection of rtN236T and rtA181V/T mutations associated with resistance to adefovir dipivoxil in samples from patients with chronic hepatitis B virus infection by the INNO- LiPA HBV DR line probe assay (version 2). J. Clin. Microbiol. 44: 1994-1997.
13. Perz JF, Armstrong GL, Farrington LA, Hutin YJ, et al. (2006). The contributions of hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer worldwide. J. Hepatol. 45: 529-538.
14. Santantonio T, Fasano M, Durantel S, Barraud L, et al. (2009). Adefovir dipivoxil resistance patterns in patients with lamivudine-resistant chronic hepatitis B. Antivir. Ther. 14: 557-565.
15. Tran N, Berne R, Chann R, Gauthier M, et al. (2006). European multicenter evaluation of high-density DNA probe arrays for detection of hepatitis B virus resistance mutations and identification of genotypes. J. Clin. Microbiol. 44: 2792-2800.
16. Villet S, Pichoud C, Billioud G, Barraud L, et al. (2008). Impact of hepatitis B virus rtA181V/T mutants on hepatitis B treatment failure. J. Hepatol. 48: 747-755.
17. Wang YZ, Xiao JH, Ruan LH, Zhang HY, et al. (2009). Detection of the rtA181V/T and rtN236T mutations associated with resistance to adefovir dipivoxil using a ligase detection reaction assay. Clin. Chim. Acta 408: 70-74.
18. Zhao Y, Zhang XY, Guo JJ, Zeng AZ, et al. (2010). Simultaneous genotyping and quantification of hepatitis B virus for genotypes B and C by real-time PCR assay. J. Clin. Microbiol. 48: 3690-3697.