Stable expression and integrated hepatitis B virus genome in a human hepatoma cell line

Genetics and Molecular Research

Volumn 11, Issue 2, 2012, Pages 1442-1448

  Wang, L Y ^a   Li, Y G ^a   Chen, K ^a   Li, K ^a   Qu, J L ^a   Qin, D D ^a   Tang, H ^a  

^a CHONGQING MEDICAL UNIVERSITY   (China)

Author keywords

Hepatitis B virus; HepG2 cell; Hygromycin; Stable cell line

Indexed keywords

CELL DNA; HEPATITIS B SURFACE ANTIGEN; HEPATITIS B(E) ANTIGEN; HYGROMYCIN; PLASMID VECTOR; VIRUS DNA;

ANTIGEN EXPRESSION; ARTICLE; CELL STRAIN HEPG2; CHROMOSOME; CONTROLLED STUDY; DNA INTEGRATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE DOSAGE; GENE EXPRESSION PROFILING; GENETIC STABILITY; GENETIC TRANSFECTION; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; IN VITRO STUDY; NONHUMAN; RECOMBINANT PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; VIRUS GENOME;

EID: 84930474949     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: 10.4238/2012.May.18.3     Document Type: Article

References (10)

1. Ganem D, Weiser B, Barchuk A, Brown RJ, et al. (1982). Biological characterization of acute infection with ground squirrel hepatitis virus. J. Virol. 44: 366-373.
2. Halpern MS, England JM, Flores L, Egan J, et al. (1984). Individual cells in tissues of DHBV-infected ducks express antigens crossreactive with those on virus surface antigen particles and immature viral cores. Virology 137: 408-413.
3. Knowles BB, Howe CC and Aden DP (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.
4. Lee W (1997). Hepatitis B virus infection. N. Engl. J. Med. 337: 1733-1745.
5. Lindberg J, Pichoud C, Hantz O, Vitvitski L, et al. (1985). Woodchuck hepatitis virus infection: serologic and histopathologic course and outcome. Eur. J. Clin. Microbiol. 4: 59-61.
6. Ochiya T, Tsurimoto T, Ueda K, Okubo K, et al. (1989). An in vitro system for infection with hepatitis B virus that uses primary human fetal hepatocytes. Proc. Natl. Acad. Sci. U. S. A. 86: 1875-1879.
7. Sells MA, Chen ML and Acs G (1987). Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA. Proc. Natl. Acad. Sci. U. S. A. 84: 1005-1009.
8. Sells MA, Zelent AZ, Shvartsman M and Acs G (1988). Replicative intermediates of hepatitis B virus in HepG2 cells that produce infectious virions. J. Virol. 62: 2836-2844.
9. Weiss L, Kekule AS, Jakubowski U, Burgelt E, et al. (1996). The HBV-producing cell line HepG2-4A5: a new in vitro system for studying the regulation of HBV replication and for screening anti-hepatitis B virus drugs. Virology 216: 214-218.
10. Will H, Cattaneo R, Koch HG, Darai G, et al. (1982). Cloned HBV DNA causes hepatitis in chimpanzees. Nature 299: 740-742.