Deletion of odhA or pyc improves production of γ-aminobutyric acid and its precursor l-glutamate in recombinant Corynebacterium glutamicum

Biotechnology Letters

Volumn 37, Issue 7, 2015, Pages 1473-1481

  Wang, Nannan ^a   Ni, Yalan ^a   Shi, Feng ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Corynebacterium glutamicum; Glutamate decarboxylase; l glutamate; odhA; pyc; Aminobutyric acid

Indexed keywords

GENES; METABOLIC ENGINEERING;

AMINOBUTYRIC ACIDS; CORYNEBACTERIUM GLUTAMICUM; GLUTAMATE DECARBOXYLASE; L-GLUTAMATE; ODHA; PYC;

AMINO ACIDS;

CORYNEBACTERIUM GLUTAMICUM;

4 AMINOBUTYRIC ACID; BACTERIAL PROTEIN; GLUTAMATE DECARBOXYLASE; GLUTAMIC ACID; RECOMBINANT PROTEIN;

CORYNEBACTERIUM GLUTAMICUM; FERMENTATION; GENE INACTIVATION; GENETICS; METABOLIC ENGINEERING; METABOLISM;

BACTERIAL PROTEINS; CORYNEBACTERIUM GLUTAMICUM; FERMENTATION; GAMMA-AMINOBUTYRIC ACID; GENE KNOCKOUT TECHNIQUES; GLUTAMATE DECARBOXYLASE; GLUTAMIC ACID; METABOLIC ENGINEERING; RECOMBINANT PROTEINS;

EID: 84930373448     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-015-1822-4     Document Type: Article

References (13)

1. Asakura Y, Kimura E, Usuda Y, Kawahara Y, Matsui K, Osumi T, Nakamatsu T (2007) Altered metabolic flux due to deletion of odhA causes l-glutamate overproduction in Corynebacterium glutamicum. Appl Environ Microbiol 73:1308–1319
2. Hu JY, Tan YZ, Li YY, Hu XQ, Xu DQ, Wang XY (2013) Construction and application of an efficient multiple-gene-deletion system in Corynebacterium glutamicum. Plasmid 70:303–313
3. Hu JY, Li YY, Zhang HL, Tan YZ, Wang XY (2014) Construction of a novel expression system for use in Corynebacterium glutamicum. Plasmid 75:18–26
4. Li HX, Qiu T, Gao DD, Cao YS (2010a) Medium optimization for production of gamma-aminobutyric acid by Lactobacillus brevis NCL912. Amino Acids 38:1439–1445
5. Li HX, Qiu T, Huang GD, Cao YS (2010b) Production of gamma-aminobutyric acid by Lactobacillus brevis NCL912 using fed-batch fermentation. Microb Cell Fact 9:85
6. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2–∆∆CT method. Methods 25:402–408
7. Park KB, Oh SH (2007) Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK-3. Bioresour Technol 98:312–319
8. Shi F, Li YX (2011) Synthesis of γ-aminobutyric acid by expressing Lactobacillus brevis-derived glutamate decarboxylase in the Corynebacterium glutamicum strain ATCC 13032. Biotechnol Lett 33:2469–2474
9. Shi F, Jiang JJ, Li YF, Li YX, Xie YL (2013) Enhancement of γ-aminobutyric acid production in recombinant Corynebacterium glutamicum by co-expressing two glutamate decarboxylase genes from Lactobacillus brevis. J Ind Microbiol Biotechnol 40:1285–1296
10. Shi F, Xie YL, Jiang JJ, Wang NN, Li YF, Wang XY (2014) Directed evolution and mutagenesis of glutamate decarboxylase from Lactobacillus brevis Lb85 to broaden the range of its activity toward a near-neutral pH. Enzyme Microb Technol 61–62:35–43
11. Shiio I, Ozaki H (1970) Regulation of nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase from Brevibacterium flavum, a glutamate-producing bacterium. J Biochem 68:633–647
12. Shirai T, Fujimura K, Furusawa C, Nagahisa K, Shioya S, Shimizu H (2007) Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis. Microb Cell Fact 6:19
13. Yao WJ, Deng XZ, Zhong H, Liu M, Zheng P, Sun ZH, Zhang Y (2009) Double deletion of dtsR1 and pyc induce efficient l-glutamate overproduction in Corynebacterium glutamicum. J Ind Microbiol Biotechnol 36:911–921