Electrophoretic mobility shift assay of DNA and CRISPR- Cas ribonucleoprotein complexes

Methods in Molecular Biology

Volumn 1311, Issue , 2015, Pages 171-184

  Künne, Tim ^a   Westra, Edze R ^a   Brouns, Stan J J ^a  

^a NONE

Author keywords

Affi nity; Binding assay; Cascade; CRISPR; Electrophoretic mobility shift assay; EMSA; Gel shift; Protein DNA interaction; RNA guide

Indexed keywords

AGAROSE; NUCLEIC ACID; OLIGONUCLEOTIDE; PLASMID DNA; RIBONUCLEOPROTEIN; DNA; ESCHERICHIA COLI PROTEIN;

AGAR GEL ELECTROPHORESIS; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; CRISPR CAS SYSTEM; DISSOCIATION CONSTANT; DNA FOOTPRINTING; DNA PROTEIN COMPLEX; ESCHERICHIA COLI; GEL; GEL MOBILITY SHIFT ASSAY; IMAGE ANALYSIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN NUCLEIC ACID INTERACTION; QUANTITATIVE ANALYSIS; GENETICS; IMAGE PROCESSING; METABOLISM; PLASMID; PROCEDURES; STAINING;

ESCHERICHIA COLI;

CRISPR-CAS SYSTEMS; DNA; ELECTROPHORETIC MOBILITY SHIFT ASSAY; ESCHERICHIA COLI PROTEINS; IMAGE PROCESSING, COMPUTER-ASSISTED; OLIGONUCLEOTIDES; PLASMIDS; RIBONUCLEOPROTEINS; STAINING AND LABELING;

EID: 84929629524     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2687-9_11     Document Type: Article

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