A novel and fully scalable Agrobacterium spray-based process for manufacturing cellulases and other cost-sensitive proteins in plants

Plant Biotechnology Journal

Volumn 13, Issue 5, 2015, Pages 708-716

  Hahn, Simone ^a   Giritch, Anatoli ^a   Bartels, Doreen ^a   Bortesi, Luisa ^a,b   Gleba, Yuri ^a  

^a Nomad Bioscience GmbH   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Nicotiana; Agrobacterium; Bioethanol; Cellulases; Griffithsin; Thaumatin

Indexed keywords

AGROBACTERIUM; NICOTIANA;

BACTERIAL DNA; CELLULASE; PLANT PROTEIN; RECOMBINANT PROTEIN; T-DNA;

AGROBACTERIUM TUMEFACIENS; BIOMASS; BIOTECHNOLOGY; GENE VECTOR; GENETICS; METABOLISM; PROCEDURES; REPLICON; TOBACCO; TRANSGENIC PLANT;

AGROBACTERIUM TUMEFACIENS; BIOMASS; BIOTECHNOLOGY; CELLULASES; DNA, BACTERIAL; GENETIC VECTORS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; REPLICON; TOBACCO;

EID: 84929511874     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/pbi.12299     Document Type: Article

References (31)

1. Azhakanandam, K., Weissinger, S.M., Nicholson, J.S., Qu, R. and Weissinger, A.K. (2007) Amplicon-plus targeting technology (APTT) for rapid production of a highly unstable vaccine protein in tobacco plants. Plant Mol. Biol. 63, 393-404.
2. Bendandi, M., Marillonnet, S., Kandzia, R., Thieme, F., Nickstadt, A., Herz, S., Fröde, R., Inogés, S., Lòpez-Dìaz de Cerio, A., Soria, E., Villanueva, H., Vancanneyt, G., McCormick, A., Tusé, D., Lenz, J., Butler-Ransohoff, J.E., Klimyuk, V. and Gleba, Y. (2010) Rapid, high-yield production in plants of individualized idiotype vaccines for non-Hodgkin's lymphoma. Ann. Oncol. 21, 2420-2427.
3. Bertani, G. (1951) Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62, 293-300.
4. Carroll, A. and Somerville, C. (2009) Cellulosic Biofuels. Annu. Rev. Plant Biol. 60, 165-182.
5. Chung, M.H., Chen, M.K. and Pan, S.M. (2000) Floral spray transformation can efficiently generate Arabidopsis transgenic plants. Transgenic Res. 9, 471-476.
6. Edens, L., Heslinga, L., Klok, R., Ledeboer, A.M., Maat, J., Toonen, M.Y., Visser, C. and Verrips, C.T. (1982) Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Escherichia coli. Gene, 18, 1-12.
7. Faus, I. and Sisniega, H. (2003) Sweet-tasting proteins. In Biopolymers, Volume 8, Polyamides and Complex Proteinaceous Materials II (Fahnestock, S.R. and Steinbüchel, A., eds), pp. 203-222. Hoboken, NJ: Wiley-Blackwell.
8. Ghose, T.K. (1987) Measurement of cellulase activities. Pure Appl. Chem. 59, 257-268.
9. Giritch, A., Marillonnet, S., Engler, C., van Eldik, G., Botterman, J., Klimyuk, V. and Gleba, Y. (2006) Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectors. Proc. Natl Acad. Sci. USA, 103, 14701-14706.
10. Gleba, Y.Y. and Giritch, A. (2012) Vaccines, antibodies, and pharmaceutical proteins. In Plant Biotechnology and Agriculture. Prospects for the 21st century (Altman, A. and Hasegawa, P.M., eds), pp. 465-479. San Diego, California, USA: Academic Press.
11. Gray, B.N., Yang, H., Ahner, B.A. and Hanson, M.R. (2011) An efficient downstream box fusion allows high-level accumulation of active bacterial beta-glucosidase in tobacco chloroplasts. Plant Mol. Biol. 76, 345-355.
12. Harvey, L.T. (1998) A Guide to Agricultural Spray Adjuvants Used in the United States. Fresno, California, USA: Thompson publications.
13. Hood, E.E., Love, R., Lane, J., Bray, J., Clough, R., Pappu, K., Drees, C., Hood, K.R., Yoon, S., Ahmad, A. and Howard, J.A. (2007) Subcellular targeting is a key condition for high-level accumulation of cellulase protein in transgenic maize seed. Plant Biotechnol. J. 5, 709-719.
14. Jung, S., Kim, S., Bae, H., Lim, H.S. and Bae, H.J. (2010) Expression of thermostable bacterial β-glucosidase (BglB) in transgenic tobacco plants. Bioresour. Technol. 101, 7155-7161.
15. Kalthoff, D., Giritch, A., Geisler, K., Bettmann, U., Klimyuk, V., Hehnen, H.R., Gleba, Y. and Beer, M. (2010) Immunization with plant-expressed hemagglutinin protects chickens from lethal highly pathogenic avian influenza virus H5N1 challenge infection. J. Virol. 84, 12002-12010.
16. Kim, S., Lee, D.S., Choi, I.S., Ahn, S.J., Kim, J.H. and Bae, H.J. (2010) Arabidopsis thaliana Rubisco small subunit transit peptide increases the accumulation of Thermotoga maritima endoglucanase Cel5A in chloroplasts of transgenic tobacco plants. Transgenic Res. 19, 489-497.
17. Mahadevan, S.A., Wi, S.G., Kim, Y.O., Lee, K.H. and Bae, H.J. (2011) In planta differential targeting analysis of Thermotoga maritima Cel5A and CBM6-engineered Cel5A for autohydrolysis. Transgenic Res. 20, 877-886.
18. Marillonnet, S., Giritch, A., Gils, M., Kandzia, R., Klimyuk, V. and Gleba, Y. (2004) In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium. Proc. Natl Acad. Sci. USA, 101, 6852-6857.
19. Marillonnet, S., Thoeringer, C., Kandzia, R., Klimyuk, V. and Gleba, Y. (2005) Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat. Biotechnol. 23, 718-723.
20. Merino, S.T. and Cherry, J. (2007) Progress and challenges in enzyme development for biomass utilization. Adv. Biochem. Eng. Biotechnol. 108, 95-120.
21. O'Keefe, B.R., Vojdani, F., Buffa, V., Shattock, R.J., Montefiori, D.C., Bakke, J., Mirsalis, J., d'Andrea, A.L., Hume, S.D., Bratcher, B., Saucedo, C.J., McMahon, J.B., Pogue, G.P. and Palmer, K.E. (2009) Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component. Proc. Natl Acad. Sci. USA, 106, 6099-6104.
22. O'Keefe, B.R., Giomarelli, B., Barnard, D.L., Shenoy, S.R., Chan, P.K., McMahon, J.B., Palmer, K.E., Barnett, B.W., Meyerholz, D.K., Wohlford-Lenane, C.L. and McCray, P.B.Jr.. (2010) Broad-spectrum in vitro activity and in vivo efficacy of the antiviral protein griffithsin against emerging viruses of the family Coronaviridae. J. Virol. 84, 2511-2521.
23. Petersen, K. and Bock, R. (2011) High-level expression of a suite of thermostable cell wall-degrading enzymes from the chloroplast genome. Plant Mol. Biol. 76, 311-321.
24. Ryu, C.M., Anand, A., Kang, L. and Mysore, K.S. (2004) Agrodrench: a novel and effective agroinoculation method for virus-induced gene silencing in roots and diverse Solanaceous species. Plant J. 40, 322-331.
25. Sainz, M.B. (2009) Commercial cellulosic ethanol: The role of plant-expressed enzymes. In Vitro Cell Dev. Biol. Plant. 45, 314-329.
26. Sticklen, M.B. (2008) Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol. Nat. Rev. Genet. 9, 433-443.
27. Takashima, S., Nakamura, A., Hidaka, M., Masaki, H. and Uozumi, T. (1996) Cloning, sequencing, and expression of the cellulase genes of Humicola grisea var. thermoidea. J. Biotechnol. 50, 137-147.
28. Takashima, S., Nakamura, A., Hidaka, M., Masaki, H. and Uozumi, T. (1999) Molecular cloning and expression of the novel fungal β-Glucosidase genes from Humicola grisea and Trichoderma reesei. J. Biochem. 125, 728-736.
29. Yu, L.X., Gray, B.N., Rutzke, C.J., Walker, L.P., Wilson, D.B. and Hanson, M.R. (2007) Expression of thermostable microbial cellulases in the chloroplasts of nicotine-free tobacco. J. Biotechnol. 131, 362-369.
30. Zeitlin, L., Pauly, M. and Whaley, K.J. (2009) Second-generation HIV microbicides: continued development of griffithsin. Proc. Natl Acad. Sci. USA, 106, 6029-6030.
31. Zhang, S., Lao, G. and Wilson, D.B. (1995) Characterization of a Thermomonospora fusca exocellulase. Biochemistry, 34, 3386-3395.