Development of a Dry-Reagent-Based qPCR to Facilitate the Diagnosis of Mycobacterium ulcerans Infection in Endemic Countries

PLoS Neglected Tropical Diseases

Volumn 9, Issue 4, 2015, Pages

  Babonneau, Jérémie ^a   Bernard, Christian ^b   Marion, Estelle ^c,d   Chauty, Annick ^b,c,i   Kempf, Marie ^d   Robert, Raymond ^b   Vincent, Quentin B ^e,f   Ab, Laurent ^e,f,g   Johnson, Christian ^b   Alcaïs, Alexandre ^e,f,g,h   Cottin, Jane ^d   Saint André, Jean Paul ^d   Marsollier, Laurent ^a,d   Ardant, Marie Françoise ^b,i   Adeye, Ambroise ⁱ   Goundote, Aimé ⁱ   Agossadou, Didier ^j  

^a UNIVERSITÉ D'ANGERS   (France)

^b SR2B ^*  (France)

^c Centre de Diagnostic et de Traitement de l'ulcère de Buruli Fondation Raoul Follereau   (Benin)

^d ANGERS UNIVERSITY HOSPITAL   (France)

^e IMAGINE INSTITUTE   (France)

^f UNIVERSITÉ PARIS DESCARTES   (France)

^g ROCKEFELLER UNIVERSITY   (United States)

^h ASSISTANCE PUBLIQUE HÔPITAUX DE PARIS   (France)

ⁱ Centre de Dépistage et de Traitement de l Ulcère de Buruli CDTUB   (Benin)

^j MINISTÈRE DE LA SANTÉ   (Benin)

more..

Author keywords

[No Author keywords available]

Indexed keywords

CRYOPROTECTIVE AGENT; DIAGNOSTIC AGENT; BACTERIAL DNA;

ARTICLE; BURULI ULCER; DNA DETERMINATION; FINE NEEDLE ASPIRATION BIOPSY; GENE AMPLIFICATION; HEAT TOLERANCE; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; QUALITY CONTROL; QUANTITATIVE ASSAY; SENSITIVITY ANALYSIS; VALIDATION PROCESS; COMPARATIVE STUDY; EVALUATION STUDY; FEMALE; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MYCOBACTERIUM ULCERANS; NEGLECTED DISEASES; PROCEDURES;

BURULI ULCER; DNA, BACTERIAL; FEMALE; HUMANS; MYCOBACTERIUM ULCERANS; NEGLECTED DISEASES; POLYMERASE CHAIN REACTION;

EID: 84929500896     PISSN: 19352727     EISSN: 19352735     Source Type: Journal    
DOI: 10.1371/journal.pntd.0003606     Document Type: Article

References (17)

1. Vincent QB, Ardant MF, Adeye A, Goundote A, Saint-Andre JP, Cottin J, et al. (2014) Clinical epidemiology of laboratory-confirmed Buruli ulcer in Benin: a cohort study. Lancet Glob Health 2: e422–430. doi: 10.1016/S2214-109X(14)70223-2 25103396
2. Asiedu K, Sherpbier R, Raviglione MC, (2000) Buruli Ulcer Mycobacterium ulcerans infection. W.H.O. Global Buruli Ulcer initiative. Report 2000 World Health Organisation Geneva Switzerland.
3. Johnson PD, Stinear T, Small PL, Pluschke G, Merritt RW, Portaels F, et al. (2005) Buruli ulcer (M. ulcerans infection): new insights, new hope for disease control. PLoS Med 2: e108. 15839744
4. Chauty A, Ardant MF, Adeye A, Euverte H, Guedenon A, Johnson C, et al. (2007) Promising clinical efficacy of streptomycin-rifampin combination for treatment of buruli ulcer (Mycobacterium ulcerans disease). Antimicrob Agents Chemother 51: 4029–4035. 17526760
5. Chauty A, Ardant MF, Marsollier L, Pluschke G, Landier J, Adeye A, et al. (2011) Oral treatment for Mycobacterium ulcerans infection: results from a pilot study in Benin. Clin Infect Dis 52: 94–96. doi: 10.1093/cid/ciq072 21148526
6. Portaels F, Agular J, Fissette K, Fonteyne PA, De Beenhouwer H, de Rijk P, et al. (1997) Direct detection and identification of Mycobacterium ulcerans in clinical specimens by PCR and oligonucleotide-specific capture plate hybridization. J Clin Microbiol 35: 1097–1100. 9114387
7. Rondini S, Mensah-Quainoo E, Troll H, Bodmer T, Pluschke G, (2003) Development and application of real-time PCR assay for quantification of Mycobacterium ulcerans DNA. J Clin Microbiol 41: 4231–4237. 12958250
8. Marion E, Ganlonon L, Claco E, Blanchard S, Kempf M, Adeye A, et al. (2014) Establishment of qPCR and culture laboratory facilities in a field hospital in Benin: one year results. Journal of clinical Microbiology 52: 4398–4400 doi: 10.1128/JCM.02131-14 25320228
9. Portaels F, WorldHealthOrganization (2014) Laboratory diagnosis of buruli ulcer; A manual for health care providers. In: Organization WH, editor. Geneva: World Health Organization. pp. 105.
10. WorldHealthOrganization (2013) Guidance on sampling techniques for laboratory-confirmation of Mycobacterium ulcerans infection (Buruli ulcer disease). Geneva: WHO.
11. Siegmund V, Adjei O, Nitschke J, Thompson W, Klutse E, Herbinger KH, et al. (2007) Dry reagent-based polymerase chain reaction compared with other laboratory methods available for the diagnosis of Buruli ulcer disease. Clin Infect Dis 45: 68–75. 17554703
12. Siegmund V, Adjei O, Racz P, Berberich C, Klutse E, van Vloten F, et al. (2005) Dry-reagent-based PCR as a novel tool for laboratory confirmation of clinically diagnosed Mycobacterium ulcerans-associated disease in areas in the tropics where M. ulcerans is endemic. J Clin Microbiol 43: 271–276. 15634982
13. Rondini S, Mensah-Quainoo E, Junghanss T, Pluschke G, (2006) What does detection of Mycobacterium ulcerans DNA in the margin of an excised Buruli ulcer lesion tell us? J Clin Microbiol 44: 4273–4275. 16928966
14. Cassisa V, Chauty A, Marion E, Ardant MF, Eyangoh S, Cottin J, et al. (2010) Use of fine-needle aspiration for diagnosis of Mycobacterium ulcerans infection. J Clin Microbiol 48: 2263–2264. doi: 10.1128/JCM.00558-10 20375229
15. Marion E, Eyangoh S, Yeramian E, Doannio J, Landier J, Aubry J, et al. (2010) Seasonal and regional dynamics of M. ulcerans transmission in environmental context: deciphering the role of water bugs as hosts and vectors. PLoS Negl Trop Dis 4: e731. doi: 10.1371/journal.pntd.0000731 20625552
16. WorldHealthOrganization, editor (2012) Treatment of Mycobacterium ulcerans (Buruli ulcer): Guidance for health workers. Geneva. 73 p.
17. Etuaful S, Carbonnelle B, Grosset J, Lucas S, Horsfield C, Phillips R, et al. (2005) Efficacy of the combination rifampin-streptomycin in preventing growth of Mycobacterium ulcerans in early lesions of Buruli ulcer in humans. Antimicrob Agents Chemother 49: 3182–3186. 16048922