A simple method for encapsulating single cells in alginate microspheres allows for direct PCR and whole genome amplification

PLoS ONE

Volumn 10, Issue 2, 2015, Pages

  Bigdeli, Saharnaz ^a,b   Dettloff, Roger O ^a,c   Frank, Curtis W ^b   Davis, Ronald W ^a   Crosby, Laurel D ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Stanford University   (United States)

^c Caerus Molecular Diagnostics   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALGINATE MICROSPHERE; BACTERIAL DNA; MICROSPHERE; UNCLASSIFIED DRUG; ALGINIC ACID; GLUCURONIC ACID; HEXURONIC ACID; MICROCAPSULE;

AEROSOL; ARTICLE; BACTERIAL CELL; BACTERIAL GENOME; BACTERIUM ISOLATE; CELL ENCAPSULATION; CONTROLLED STUDY; CYTOLYSIS; DNA EXTRACTION; FEASIBILITY STUDY; FLUORESCENCE MICROSCOPY; GENE AMPLIFICATION; GENE DOSAGE; MICROSCOPY; MULTIPLE DISPLACEMENT AMPLIFICATION; NEXT GENERATION SEQUENCING; NONHUMAN; POLYMERASE CHAIN REACTION; RHODOBACTER SPHAEROIDES; SEQUENCE ALIGNMENT; WHOLE GENOME AMPLIFICATION; CELL SEPARATION; CHEMISTRY; CYTOLOGY; DNA SEQUENCE; GENETICS; MICROCAPSULE; PROCEDURES;

RHODOBACTER SPHAEROIDES;

ALGINATES; CAPSULES; CELL SEPARATION; FEASIBILITY STUDIES; GENOME, BACTERIAL; GLUCURONIC ACID; HEXURONIC ACIDS; MICROSPHERES; POLYMERASE CHAIN REACTION; RHODOBACTER SPHAEROIDES; SEQUENCE ANALYSIS, DNA;

EID: 84928914571     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0117738     Document Type: Article

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