Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus

Veterinary Microbiology

Volumn 177, Issue 3-4, 2015, Pages 373-378

  Jung, Kwonil ^a   Eyerly, Bryan ^a   Annamalai, Thavamathi ^a   Lu, Zhongyan ^a   Saif, Linda J ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

Adherens junction; PEDV, Tight junction; Pig; Porcine epidemic diarrhea virus; Virus

Indexed keywords

OCCLUDIN; UNCLASSIFIED DRUG; UVOMORULIN; VIRUS ANTIGEN; ZONULA OCCLUDIN 1; CADHERIN; PROTEIN ZO1;

ANIMAL TISSUE; ANTIGEN DETECTION; ARTICLE; CELL JUNCTION; CELL STRUCTURE; CONTROLLED STUDY; DIARRHEA; ENTERITIS; ILEUM; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; INTESTINE EPITHELIUM CELL; JEJUNUM; LARGE INTESTINE; NONHUMAN; PIGLET; PORCINE EPIDEMIC DIARRHEA; PORCINE EPIDEMIC DIARRHEA VIRUS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SMALL INTESTINE; TIGHT JUNCTION; VIRUS DETECTION; VIRUS STRAIN; VOMITING; ANIMAL; CORONAVIRUS INFECTION; EPITHELIUM; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GERMFREE ANIMAL; IMMUNOLOGY; INTESTINE; PATHOLOGY; PIG; RANDOMIZATION; SUCKLING ANIMAL; SWINE DISEASE; VETERINARY; VIROLOGY;

BACTERIA (MICROORGANISMS); PORCINE EPIDEMIC DIARRHEA VIRUS; SUIDAE;

ADHERENS JUNCTIONS; ANIMALS; ANIMALS, SUCKLING; ANTIGENS, VIRAL; CADHERINS; CORONAVIRUS INFECTIONS; EPITHELIUM; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOHISTOCHEMISTRY; INTESTINE, LARGE; INTESTINE, SMALL; INTESTINES; PORCINE EPIDEMIC DIARRHEA VIRUS; RANDOM ALLOCATION; SPECIFIC PATHOGEN-FREE ORGANISMS; SWINE; SWINE DISEASES; TIGHT JUNCTIONS; ZONULA OCCLUDENS-1 PROTEIN;

EID: 84928828905     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2015.03.022     Document Type: Article

References (13)

1. Chung C.Y., Alden S.L., Funderburg N.T., Fu P., Levine A.D. Progressive proximal-to-distal reduction in expression of the tight junction complex in colonic epithelium of virally-suppressed HIV+ individuals. PLoS Pathog. 2014, 10:e1004198.
2. Hartsock A., Nelson W.J. Adherens and tight junctions: structure, function and connections to the actin cytoskeleton. Biochim. Biophys. Acta 2008, 1778:660-669.
3. Jung K., Kim J., Ha Y., Choi C., Chae C. The effects of transplacental porcine circovirus type 2 infection on porcine epidemic diarrhoea virus-induced enteritis in preweaning piglets. Vet. J. 2006, 171:445-450.
4. Jung K., Renukaradhya G.J., Alekseev K.P., Fang Y., Tang Y., Saif L.J. Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections. J. Gen. Virol. 2009, 90:2713-2723.
5. Jung K., Saif L.J. Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis. Vet. J. 2015, (in press). 10.1016/j.tvjl.2015.02.017.
6. Jung K., Wang Q., Scheuer K.A., Lu Z., Zhang Y., Saif L.J. Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs. Emerg. Infect. Dis. 2014, 20:662-665.
7. Nicholls J., Dong X.P., Jiang G., Peiris M. SARS: clinical virology and pathogenesis. Respirology 2003, 8(Suppl.):S6-S8.
8. Saif L.J., Pensaert M.P., Sestak K., Yeo S.G., Jung K. Coronaviruses. Diseases of Swine 2012, 501-524. Wiley-Blackwell, Iowa State University. J.J. Zimmerman, L.A. Karriker, A. Ramirez, K.J. Schwartz, G.W. Stevenson (Eds.).
9. Su C.W., Cao Y., Kaplan J., Zhang M., Li W., Conroy M., Walker W.A., Shi H.N. Duodenal helminth infection alters barrier function of the colonic epithelium via adaptive immune activation. Infect. Immun. 2011, 79:2285-2294.
10. Teoh K.T., Siu Y.L., Chan W.L., Schluter M.A., Liu C.J., Peiris J.S., Bruzzone R., Margolis B., Nal B. The SARS coronavirus E protein interacts with PALS1 and alters tight junction formation and epithelial morphogenesis. Mol. Biol. Cell 2010, 21:3838-3852.
11. Turgeon D.C., Morin M., Jolette J., Higgins R., Marsolais G., DiFranco E. Coronavirus-like particles associated with diarrhea in baby pigs in Quebec. Can. Vet. J. 1980, 21:100-110.
12. Wang J., Zhao P., Guo L., Liu Y., Du Y., Ren S., Li J., Zhang Y., Fan Y., Huang B., Liu S., Wu J. Porcine epidemic diarrhea virus variants with high pathogenicity, China. Emerg. Infect. Dis. 2013, 19:2048-2049.
13. Zhao S., Gao J., Zhu L., Yang Q. Transmissible gastroenteritis virus and porcine epidemic diarrhoea virus infection induces dramatic changes in the tight junctions and microfilaments of polarized IPEC-J2 cells. Virus Res. 2014, 192:34-45.