Mapping protein-protein interactions using yeast two-hybrid assays

Cold Spring Harbor Protocols

Volumn 2015, Issue 5, 2015, Pages 442-452

  Mehla, Jitender ^a   Caufield, J Harry ^a   Uetz, Peter ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CARDIOVASCULAR EQUIPMENT; CRUSH TRAUMA; EMERGENCY WARD; EXERCISE; FALLING; HEALTH CENTER; HUMAN; INJURY; OVEREXERTION; PHYSICAL ACTIVITY; GENETIC SCREENING; GENETICS; METABOLISM; PROCEDURES; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; TWO HYBRID SYSTEM;

RECOMBINANT PROTEIN;

GENETIC TESTING; PROTEIN INTERACTION MAPS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 84928790141     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot086157     Document Type: Article

References (18)

1. Chen YC, Rajagopala SV, Stellberger T, Uetz P. 2010. Exhaustive benchmarking of the yeast two-hybrid system. Nat Methods 7: 667-668.
2. Gong W, Shen YP, Ma LG, Pan Y, Du YL, Wang DH, Yang JY, Hu LD, Liu XF, Dong CX, et al. 2004. Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes. Plant Physiol 135: 773-782.
3. Harper JW, Adami GR, Wei N, Keyomarsi K, Elledge SJ. 1993. The p21 Cdkinteracting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816.
4. James P, Halladay J, Craig EA. 1996. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144: 1425-1436.
5. Lamesch P, Milstein S, Hao T, Rosenberg J, Li N, Sequerra R, Bosak S, Doucette-Stamm L, Vandenhaute J, Hill DE, et al. 2004. C. elegans ORFeome version 3.1: Increasing the coverage of ORFeome resources with improved gene predictions. Genome Res 14: 2064-2069.
6. Landy A. 1989. Dynamic, structural, and regulatory aspects of λ site-specific recombination. Annu Rev Biochem 58: 913-949.
7. Mehla J, Caufield JH, Uetz P. 2015. The yeast two-hybrid system: A tool for mapping protein-protein interactions. Cold Spring Harb Protoc doi: 10.1101/pdb.top083345.
8. Rain JC, Selig L, De Reuse H, Battaglia V, Reverdy C, Simon S, Lenzen G, Petel F, Wojcik J, Schachter V, et al. 2001. The protein-protein interaction map of Helicobacter pylori. Nature 409: 211-215.
9. Rajagopala SV, Uetz P. 2011. Analysis of protein-protein interactions using high-throughput yeast two-hybrid screens. Methods Mol Biol 781: 1-29.
10. Rajagopala SV, Titz B, Uetz P. 2007. Array-based yeast two-hybrid screening for protein-protein interactions. In Yeast gene analysis, 2nd ed. (Methods in Microbiology Series) (Stansfield I, Stark MJ), Vol. 36, pp. 139-163. Academic, London.
11. Rajagopala SV, Yamamoto N, Zweifel AE, Nakamichi T, Huang HK, Mendez-Rios JD, Franca-Koh J, Boorgula MP, Fujita K, Suzuki K, et al. 2010. The Escherichia coli K-12 ORFeome: A resource for comparative molecular microbiology. BMC Genomics 11: 470.
12. Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, et al. 2014. The binary protein-protein interaction landscape of Escherichia coli. Nat Biotechnol 32: 285-290.
13. Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li N, Rosenberg J, Lamesch P, Vidalain PO, et al. 2004. Human ORFeome version 1.1: A platform for reverse proteomics. Genome Res 14: 2128-2135.
14. Serebriiskii I, Golemis E, Uetz P. 2005. The yeast two-hybrid system for detecting interacting proteins. In The proteomics handbook (Walker JM), pp. 653-682. Humana, Totowa, NJ.
15. Stellberger T, Hauser R, Baiker A, Pothineni VR, Haas J, Uetz P. 2010. Improving the yeast two-hybrid system with permutated fusions proteins: The Varicella Zoster virus interactome. Proteome Sci 8: 8.
16. Uetz P. 2002. Two-hybrid arrays. Curr Opin Chem Biol 6: 57-62.
17. Uetz P, Dong YA, Zeretzke C, Atzler C, Baiker A, Berger B, Rajagopala SV, Roupelieva M, Rose D, Fossum E, et al. 2006. Herpesviral protein networks and their interaction with the human proteome. Science 311: 239-242.
18. Walhout AJ, Temple GF, Brasch MA, Hartley JL, Lorson MA, van den Heuvel S, Vidal M. 2000. GATEWAY recombinational cloning: Application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol 328: 575-592.