Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Ye, Xiaoting ^a   Morikawa, Kana ^a   Ho, Shih Hsin ^a   Araki, Michihiro ^a   Nishida, Keiji ^a   Hasunuma, Tomohisa ^a   Hara, Kiyotaka Y ^a   Kondo, Akihiko ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

ATP synthesis; High throughput analysis; Mitochondria purification

Indexed keywords

ADENOSINE TRIPHOSPHATE; FIREFLY LUCIFERASE;

ADENINE NUCLEOTIDE TRANSLOCASE GENE; ADENOSINE TRIPHOSPHATE SYNTHETASE GENE; ARTICLE; CENTRIFUGATION; CYTOCHROME BC1 COMPLEX GENE; CYTOCHROME C OXIDASE GENE; CYTOCHROME C REDUCTASE GENE; CYTOLYSIS; FUMARATE REDUCTASE GENE; GENE; GENE DELETION; MITOCHONDRION; NADH DEHYDROGENASE GENE; NONHUMAN; OXIDATIVE PHOSPHORYLATION; RESPIRATORY CHAIN; SACCHAROMYCES CEREVISIAE; SUCCINATE DEHYDROGENASE GENE; SYNTHESIS; HIGH THROUGHPUT SCREENING; METABOLISM; PROCEDURES;

SACCHAROMYCES CEREVISIAE;

ADENOSINE TRIPHOSPHATE; HIGH-THROUGHPUT SCREENING ASSAYS; MITOCHONDRIA; OXIDATIVE PHOSPHORYLATION;

EID: 84928578752     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0239-z     Document Type: Article

References (25)

1. Stump CS, Short KR, Bigelow ML, Schimke JM, Nair KS. Effect of insulin on human skeletal muscle mitochondrial ATP production, protein synthesis, and mRNA transcripts. Proc Natl Acad Sci U S A. 2003;100:7996-8001.
2. Drew B, Leeuwenburgh C. Method for measuring ATP production in isolation mitochondria: ATP production in brain and liver mitochondria of Fischer-344 rats with age and caloric restriction. Am J Physiol Regul Integr Comp Physiol. 2003;285:1259-67.
3. Fujikawa M, Yoshida M. A sensitive simple assay of mitochondria ATP synthesis of cultured mammalian cells suitable for high-throughput analysis. Biochem Biophys Res Commun. 2010;401:538-43.
4. Frezza C, Cipolat S, Scorrano L. Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts. Nat Protoc. 2007;2:287-95.
5. Gregg C, Kyryakov P, Titorenko V. Purification of mitochondria from yeast cells. J Vis Exp. 2009;30:1417.
6. Granham JM. Purification of a crude mitochondrial fraction by density-gradient centrifugation. Curr Protoc Cell Biol. 2001, doi:10.1002/0471143030.cb0304s04.
7. Daum G, Böhni PC, Schatz G. Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. J Biol Chem. 1982;257:13028-33.
8. Wibom R, Lundin A, Hultman E. A sensitive method for measuring ATP-formation in rat muscle mitochondria. Scand J Clin Lab Invest. 1990;50:143-52.
9. Han YH, Kim SH, Kim SZ, Park WH. Antimycin A as a mitochondrial electron transport inhibitor prevents the growth of human lung cancer A549 cells. Oncol Rep. 2008;20:689-93.
10. Makowska A, Zablocki K, Duszynski J. The role of mitochondria in the regulation of calcium influx into Jurkat cells. Eur J Biochem. 2000;267:877-84.
11. Yamamoto T, Terauchi S, Tachikawa A, Yamashita K, Kataoka M, Terada H, et al. Two critical factors affecting the release of mitochondrial cytochrome c as revealed by studies using N, N'-dicyclohexylcarbodiimide as an atypical inducer of permeability transition. J Bioenerg Biomembr. 2005;37:299-306.
12. Huang ME, Rio AG, Nicolas A, Kolodner RD. A genomewide screen in Sacchromyces cerevisiae for genes that suppress the accumulation of mutants. Proc Natl Acad Sci U S A. 2003;100:11529-34.
13. Birrell GW, Brown JA, Wu HI, Giaever G, Chu AM, Davis RW, et al. Transcriptional response of Saccharomyces cerevisiae to DNA-damaging agents does not identify the genes that protect against these agents. Proc Natl Acad Sci U S A. 2001;99:8778-83.
14. Chan TF, Carvalho J, Riles L, Zheng XF. A chemical genomics approach toward understanding the global functions of the target of rapamycin protein (TOR). Proc Natl Acad Sci U S A. 2000;97:13227-32.
15. Weimann T, Vaillier J, Salin B, Velours J. The intermembrane space loop of subunit b (4) is a major determinant of the stability of yeast oligomeric ATP synthases. Biochemistry. 2008;47:3556-63.
16. 1-ATPase: propagation of the inhibitory effect of the epsilon subunit. J Biol Chem. 2001;276:23969-73.
17. Gu Y, Gordon DM, Amutha B, Pain D. A GTP:AMP phosphotransferase, Adk2p, in Saccharomyces cerevisiae. Role of the C terminus in protein folding/stabilization, thermal tolerance, and enzymatic activity. J Biol Chem. 2005;280:18604-9.
18. Klingenberg M. The ADP and ATP transport in mitochondria and its carrier. Biochim Biophys Acta. 2008;1778:1978-2021.
19. Drgon T, Sabova L, Nelson N, Kolarov J. ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae. FEBS. 1991;289:159-62.
20. Dorsman JC, Grivell LA. Expression of the gene encoding subunit II of yeast QH2: cytochrome c oxidoreductase is regulated by multiple factors. Curr Genet. 1990;17:459-64.
21. Oudshoorn P, Van Steeg H, Swinkels BW, Schoppink P, Grivell LA. Subunit II of yeast QH2: cytochrome c oxidoreductase. Nucleotide sequence of the gene and features of the protein. Eur J Biochem. 1987;163:97-103.
22. Hunte C, Palsdottir H, Trumpower BL. Protonmotive pathways and mechanisms in the cytochrome bc1 complex. FEBS Lett. 2003;545:39-46.
23. Hara KY, Mori H. An efficient method for quantitative determination of cellular ATP synthetic activity. J Biomol Screen. 2006;11:310-7.
24. Hara KY. Permeable cell assay: a method for high-throughput measurement of cellular ATP synthetic activity. Methods Mol Biol. 2009;577:251-8.
25. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.