Performance evaluation of the Verigene® (Nanosphere) and FilmArray® (BioFire®) molecular assays for identification of causative organisms in bacterial bloodstream infections

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 34, Issue 3, 2015, Pages 487-496

  Ward, C ^a   Stocker, K ^b   Begum, J ^b   Wade, P ^a   Ebrahimsa, U ^a   Goldenberg, S D ^a,c  

^a GUY'S AND ST THOMAS' NHS FOUNDATION TRUST   (United Kingdom)

^b ViaPath LLP   (United Kingdom)

^c ST THOMAS HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACINETOBACTER BAUMANNII; ALPHA HEMOLYTIC STREPTOCOCCUS; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL INFECTION; BACTERIUM IDENTIFICATION; BACTEROIDES FRAGILIS; BIOASSAY; BLOOD CULTURE; BLOODSTREAM INFECTION; CANDIDA ALBICANS; CANDIDA GLABRATA; CANDIDA PARAPSILOSIS; CANDIDA TROPICALIS; CONTROLLED STUDY; CORYNEBACTERIUM; ENTEROBACTER CLOACAE; ENTEROCOCCUS AVIUM; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; FILMARRAY ASSAY; FUSOBACTERIUM NECROPHORUM; GENE IDENTIFICATION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HAEMOPHILUS INFLUENZAE; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; LISTERIA MONOCYTOGENES; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROCOCCUS LUTEUS; MORGANELLA MORGANII; NEISSERIA MENINGITIDIS; NONHUMAN; PAENIBACILLUS MACERANS; PICHIA KUDRIAVZEVII; PREVOTELLA; PRIORITY JOURNAL; PROPIONIBACTERIUM ACNES; PSEUDOMONAS AERUGINOSA; SERRATIA MARCESCENS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS; STAPHYLOCOCCUS HOMINIS; STAPHYLOCOCCUS LUGDUNENSIS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS ANGINOSUS; STREPTOCOCCUS PNEUMONIAE; STREPTOCOCCUS PYOGENES; TURNAROUND TIME; VERIGENE ASSAY; ADULT; AGED; BACTEREMIA; BACTERIUM; CLASSIFICATION; COMPARATIVE STUDY; DIAGNOSTIC ERROR; DIAGNOSTIC USE; DRUG EFFECTS; EVALUATION STUDY; FEMALE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MALE; MIDDLE AGED; MOLECULAR DIAGNOSIS; PROCEDURES; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; TIME; YOUNG ADULT;

NANOSPHERE;

ADULT; AGED; BACTEREMIA; BACTERIA; DIAGNOSTIC ERRORS; DRUG RESISTANCE, BACTERIAL; FEMALE; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; NANOSPHERES; RETROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; TIME FACTORS; YOUNG ADULT;

EID: 84928104512     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-014-2252-2     Document Type: Article

References (27)

1. McPherson D, Griffiths C, Williams M, Baker A, Klodawski E, Jacobson B, Donaldson L (2013) Sepsis-associated mortality in England: an analysis of multiple cause of death data from 2001 to 2010. BMJ Open. doi:10.1136/bmjopen-2013-002586
2. Padkin A, Goldfrad C, Brady AR, Young D, Black N, Rowan K (2003) Epidemiology of severe sepsis occurring in the first 24 hrs in intensive care units in England, Wales, and Northern Ireland. Crit Care Med 31:2332–2338
3. Dellinger RP, Levy MM, Rhodes A, Annane D, Gerlach H, Opal SM, Sevransky JE, Sprung CL, Douglas IS, Jaeschke R, Osborn TM, Nunnally ME, Townsend SR, Reinhart K, Kleinpell RM, Angus DC, Deutschman CS, Machado FR, Rubenfeld GD, Webb SA, Beale RJ, Vincent J-L, Moreno R; Surviving Sepsis Campaign Guidelines Committee including the Pediatric Subgroup (2013) Surviving sepsis campaign: international guidelines for management of severe sepsis and septic shock: 2012. Crit Care Med 41:580–637
4. Kumar A, Roberts D, Wood KE, Light B, Parrillo JE, Sharma S, Suppes R, Feinstein D, Zanotti S, Taiberg L, Gurka D, Kumar A, Cheang M (2006) Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 34:1589–1596
5. Davies SC (2013) Annual report of the Chief Medical Officer 2011: volume two, 2011. Infections and the rise of antimicrobial resistance. Department of Health, London
6. Stoneking LR, Patanwala AE, Winkler JP, Fiorello AB, Lee ES, Olson DP, Wolk DM (2013) Would earlier microbe identification alter antibiotic therapy in bacteremic emergency department patients? J Emerg Med 44:1–8
7. Pérez-Vázquez M, Oliver A, Sánchez del Saz B, Loza E, Baquero F, Cantón R (2001) Performance of the VITEK2 system for identification and susceptibility testing of routine Enterobacteriaceae clinical isolates. Int J Antimicrob Agents 17:371–376
8. Fournier P-E, Drancourt M, Colson P, Rolain J-M, La Scola B, Raoult D (2013) Modern clinical microbiology: new challenges and solutions. Nat Rev Microbiol 11:574–585
9. Peters RPH, van Agtmael MA, Danner SA, Savelkoul PHM, Vandenbroucke-Grauls CMJE (2004) New developments in the diagnosis of bloodstream infections. Lancet Infect Dis 4:751–760
10. La Scola B (2011) Intact cell MALDI-TOF mass spectrometry-based approaches for the diagnosis of bloodstream infections. Expert Rev Mol Diagn 11:287–298
11. Chang S-S, Hsieh W-H, Liu TS, Lee S-H, Wang C-H, Chou H-C, Yeo YH, Tseng C-P, Lee C-C (2013) Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis—a systemic review and meta-analysis. PLoS One 8:e62323
12. Samuel LP, Tibbetts RJ, Agotesku A, Fey M, Hensley R, Meier FA (2013) Evaluation of a microarray-based assay for rapid identification of Gram-positive organisms and resistance markers in positive blood cultures. J Clin Microbiol 51:1188–1192
13. Wojewoda CM, Sercia L, Navas M, Tuohy M, Wilson D, Hall GS, Procop GW, Richter SS (2013) Evaluation of the Verigene Gram-positive blood culture nucleic acid test for rapid detection of bacteria and resistance determinants. J Clin Microbiol 51:2072–2076
14. Buchan BW, Ginocchio CC, Manii R, Cavagnolo R, Pancholi P, Swyers L, Thomson RB Jr, Anderson C, Kaul K, Ledeboer NA (2013) Multiplex identification of gram-positive bacteria and resistance determinants directly from positive blood culture broths: evaluation of an automated microarray-based nucleic acid test. PLoS Med 10:e1001478
15. Sullivan KV, Turner NN, Roundtree SS, Young S, Brock-Haag CA, Lacey D, Abuzaid S, Blecker-Shelly DL, Doern CD (2013) Rapid detection of Gram-positive organisms by use of the Verigene Gram-positive blood culture nucleic acid test and the BacT/Alert Pediatric FAN system in a multicenter pediatric evaluation. J Clin Microbiol 51:3579–3584
16. Alby K, Daniels LM, Weber DJ, Miller MB (2013) Development of a treatment algorithm for streptococci and enterococci from positive blood cultures identified with the Verigene Gram-positive blood culture assay. J Clin Microbiol 51:3869–3871
17. Mestas J, Polanco CM, Felsenstein S, Dien Bard J (2014) Performance of the Verigene Gram-positive blood culture assay for direct detection of Gram-positive organisms and resistance markers in a pediatric hospital. J Clin Microbiol 52:283–287
18. Mancini N, Infurnari L, Ghidoli N, Valzano G, Clementi N, Burioni R, Clementi M (2014) Potential Impact of a microarray-based nucleic acid assay for rapid detection of Gram-negative bacteria and resistance markers in positive blood cultures. J Clin Microbiol 52:1242–1245
19. Altun O, Almuhayawi M, Ullberg M, Ozenci V (2013) Clinical evaluation of the FilmArray blood culture identification panel in identification of bacteria and yeasts from positive blood culture bottles. J Clin Microbiol 51:4130–4136
20. Beal SG, Ciurca J, Smith G, John J, Lee F, Doern CD, Gander RM (2013) Evaluation of the Nanosphere Verigene Gram-positive blood culture assay with the VersaTREK blood culture system and assessment of possible impact on selected patients. J Clin Microbiol 51:3988–3992
21. Sango A, McCarter YS, Johnson D, Ferreira J, Guzman N, Jankowski CA (2013) Stewardship approach for optimizing antimicrobial therapy through use of a rapid microarray assay on blood cultures positive for Enterococcus species. J Clin Microbiol 51:4008–4011
22. Scott LJ (2013) Verigene® gram-positive blood culture nucleic acid test. Mol Diagn Ther 17:117–122
23. Sullivan KV, DeBurger B, Roundtree SS, Ventrola CA, Blecker-Shelly DL, Mortensen JE (2014) Pediatric multicenter evaluation of the Verigene gram-negative blood culture test for rapid detection of inpatient bacteremia involving gram-negative organisms, extended-spectrum beta-lactamases, and carbapenemases. J Clin Microbiol 52:2416–2421. doi:10.1128/JCM.00737-14
24. McNeil MM, Davis BJ, Anderson RL, Martone WJ, Solomon SL (1985) Mechanism of cross-contamination of blood culture bottles in outbreaks of pseudobacteremia associated with nonsterile blood collection tubes. J Clin Microbiol 22:23–25
25. Noskin GA, Suriano T, Collins S, Sesler S, Peterson LR (2001) Paenibacillus macerans pseudobacteremia resulting from contaminated blood culture bottles in a neonatal intensive care unit. Am J Infect Control 29:126–129
26. Spangler R, Goddard NL, Thaler DS (2009) Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria. PLoS One 4:e7010
27. Blaschke AJ, Heyrend C, Byington CL, Fisher MA, Barker E, Garrone NF, Thatcher SA, Pavia AT, Barney T, Alger GD, Daly JA, Ririe KM, Ota I, Poritz MA (2012) Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system. Diagn Microbiol Infect Dis 74:349–355