Isolation and culture of primary pericytes from mouse brain

Methods in Molecular Biology

Volumn 1135, Issue , 2014, Pages 383-392

  Boroujerdi, Amin ^a   Tigges, Ulrich ^a   Welser Alves, Jennifer V ^a   Milner, Richard ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Brain endothelial cell (BEC); Coculture; Culture; Matrigel; Pericyte; Purity

Indexed keywords

CD146 ANTIGEN; PLATELET DERIVED GROWTH FACTOR BETA RECEPTOR; BIOLOGICAL MARKER; BRAIN PROTEIN; CD11B ANTIGEN; CD31 ANTIGEN; GLIAL FIBRILLARY ACIDIC PROTEIN; PROTEIN NG2; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL TISSUE; ARTICLE; BRAIN PERICYTE; CELL COUNT; CELL FUNCTION; CELL GROWTH; CELL ISOLATION; CELL POPULATION; CENTRAL NERVOUS SYSTEM; CULTURE OPTIMIZATION; MOUSE; NONHUMAN; PRIMARY CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; ADULT; ASTROCYTE; CELL CULTURE; ENDOTHELIUM CELL; MICROGLIA;

ANIMALS; BIOLOGICAL MARKERS; BRAIN; CELL SEPARATION; CELLS, CULTURED; IMMUNOHISTOCHEMISTRY; MICE; MICROVESSELS; PERICYTES; PRIMARY CELL CULTURE;

MUS;

EID: 84927947690     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-320-7_31     Document Type: Article

References (11)

1. Armulik A, Abramsson A, Betsholtz C (2005) Endothelial/pericyte interactions. Circ Res 97: 512-523
2. Hirschi KK, D'Amore PA (1996) Pericytes in the microvasculature. Cardiovasc Res 32:687-698
3. Bergers G, Song S (2005) The role of pericytes in blood-vessel formation and maintenance. Neuro Oncol 7:452-464
4. Dore-Duffy P, LaManna JC (2007) Physiologic angiodynamics in the brain. Antioxid Redox Signal 9:1363-1371
5. Hamilton NB, Attwell D, Hall CN (2010) Pericyte-mediated regulation of capillary diameter: a component of neurovascular coupling in health and disease. Front Neuroenergetics 2:1-14
6. Kutcher ME, Herman IM (2009) The pericyte: cellular regulator of microvascular blood flow. Microvasc Res 77:235-246
7. Herman IM, Jacobson S (1988) In situ analysis of microvascular pericytes in hypertensive rat brains. Tissue Cell 20:1-12
8. Hammes HP (2005) Pericytes and the pathogenesis of diabetic retinopathy. Horm Metab Res 37:39-43
9. Gitlin JD, D'Amore PA (1983) Culture of retinal capillary cells using selective growth media. Microvasc Res 26:74-80
10. Dore-Duffy P (2003) Isolation and characterization of cerebral microvascular pericytes. Methods Mol Med 89:375-382
11. Tigges U, Welser-Alves JV, Boroujerdi A, Milner R (2012) A novel and simple method for culturing pericytes from mouse brain. Microvasc Res 84:74-80