Human alpha-fetal protein immunoassay using fluorescence suppression with fluorescent-bead/antibody conjugate and enzymatic reaction

Biosensors and Bioelectronics

Volumn 71, Issue , 2015, Pages 115-120

  Ahn, Junhyoung ^a   Shin, Yong Beom ^b   Lee, JaeJong ^a   Kim, Min Gon ^c  

^a Korea Institute of Machine and Materials   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^c Gwangju Institute of Science and Technology (GIST)   (South Korea)

Author keywords

Alpha fetal protein (AFP); Enzyme catalyzed precipitation; Fluorescence suppression; Fluorescent immunoassay

Indexed keywords

ANTIBODIES; ANTIGENS; ENZYMES; IMMUNOLOGY; PRECIPITATES; PROTEINS;

ALPHA-FETAL PROTEIN (AFP); BIOTINYLATED ANTIBODIES; ENZYMATIC REACTION; FLUORESCENCE SUPPRESSION; FLUORESCENT IMMUNOASSAYS; FLUORESCENT IMMUNOSENSORS; HORSERADISH PEROXIDASE; OPTIMIZED CONDITIONS;

FLUORESCENCE;

ALPHA FETOPROTEIN; ANTIBODY CONJUGATE; FLUORESCENT DYE; HORSERADISH PEROXIDASE; HYDROGEN PEROXIDE;

ANTIGEN ANTIBODY REACTION; ANTIGEN BINDING; ARTICLE; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME MECHANISM; FLUORESCENCE; IMAGE ANALYSIS; IMMUNOPRECIPITATION; INTERMETHOD COMPARISON; PROTEIN DETERMINATION; SENSITIVITY AND SPECIFICITY; CHEMISTRY; DEVICES; EQUIPMENT DESIGN; EVALUATION STUDY; FLUORESCENT ANTIBODY TECHNIQUE; GENETIC PROCEDURES; HUMAN; LIMIT OF DETECTION; METABOLISM; PRECIPITATION; PROCEDURES;

ARMORACIA RUSTICANA;

ALPHA-FETOPROTEINS; BIOSENSING TECHNIQUES; CHEMICAL PRECIPITATION; EQUIPMENT DESIGN; FLUORESCENCE; FLUORESCENT ANTIBODY TECHNIQUE; HORSERADISH PEROXIDASE; HUMANS; HYDROGEN PEROXIDE; IMMUNOCONJUGATES; LIMIT OF DETECTION;

EID: 84927761447     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2015.03.073     Document Type: Article

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