Expression and purification of NifB proteins from aerobic and anaerobic sources

Methods in Molecular Biology

Volumn 1122, Issue , 2014, Pages 19-31

  Echavarri Erasun, Carlos ^a   Arragain, Simon ^a   Scandurra, Alessandro A ^a   Rubio, Luis M ^a  

^a NONE

Author keywords

Anaerobic protein purification; Azotobacter vinelandii; Escherichia coli; Glutathione S transferase (GST); Immobilized metal affinity chromatography (IMAC); Klebsiella pneumoniae; NifB; NifB co; Nitrogenase; Phenylmethylsulfonyl fluoride (PMSF); Sodium dithionite (DTH)

Indexed keywords

BACTERIAL PROTEIN; BUFFER; NIFB PROTEIN; POLYHISTIDINE TAG; PROTEINASE INHIBITOR; UNCLASSIFIED DRUG; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; NIFB PROTEIN, BACTERIA; OLIGOPEPTIDE;

ARTICLE; AZOTOBACTER VINELANDII; BACTERIAL CELL; CELL GROWTH; ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; THERMOPHILIC BACTERIUM; AEROBIC METABOLISM; AFFINITY CHROMATOGRAPHY; ANAEROBIC GROWTH; BIOCHEMISTRY; BIOSYNTHESIS; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

AZOTOBACTER VINELANDII; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE;

AEROBIOSIS; ANAEROBIOSIS; AZOTOBACTER VINELANDII; BACTERIAL PROTEINS; BIOCHEMISTRY; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; HISTIDINE; KLEBSIELLA PNEUMONIAE; OLIGOPEPTIDES; RECOMBINANT FUSION PROTEINS;

EID: 84927549441     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-794-5_3     Document Type: Article

References (11)

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