Molecular detection of leptospiral DNA in environmental water on St. Kitts

International Journal of Environmental Research and Public Health

Volumn 11, Issue 8, 2014, Pages 7953-7960

  Rawlins, Julienne ^a   Portanova, Alexandra ^a   Zuckerman, Ilana ^a   Loftis, Amanda ^a,c   Ceccato, Pietro ^b   Willingham, Arve Lee ^a   Verma, Ashutosh ^a  

^a ROSS UNIVERSITY SCHOOL OF VETERINARY MEDICINE   (Saint Kitts and Nevis)

^b Earth Institute   (United States)

^c MIDWESTERN UNIVERSITY   (United States)

Author keywords

Environmental transmission; Leptospira; Leptospirosis; Molecular detection

Indexed keywords

BACTERIAL DNA; LEPTOSPIRAL DNA; SURFACE WATER; UNCLASSIFIED DRUG; FRESH WATER; LIPL32 PROTEIN, LEPTOSPIRA; LIPOPROTEIN; OUTER MEMBRANE PROTEIN;

BACTERIAL DISEASE; BACTERIUM; DNA; GENE; MARINE ENVIRONMENT; MARINE POLLUTION; MOLECULAR ANALYSIS; PATHOGEN; WATER QUALITY; WATERBORNE DISEASE;

ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; DNA EXTRACTION; ENVIRONMENTAL IMPACT ASSESSMENT; GENE TARGETING; LEPTOSPIRA; LEPTOSPIRA INTERROGANS; LIPL32 GENE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; URINE VOLUME; WATER ANALYSIS; WATER CONTAMINATION; WATER FLOW; WATER QUALITY; WATER TEMPERATURE; CLASSIFICATION; ENVIRONMENTAL MONITORING; GENETICS; ISOLATION AND PURIFICATION; LEPTOSPIROSIS; METABOLISM; MICROBIOLOGY; PREVALENCE; SAINT KITTS AND NEVIS;

LEEWARD ISLANDS [LESSER ANTILLES]; SAINT KITTS; SAINT KITTS AND NEVIS;

BACTERIAL OUTER MEMBRANE PROTEINS; ENVIRONMENTAL MONITORING; FRESH WATER; LEPTOSPIRA; LEPTOSPIROSIS; LIPOPROTEINS; PREVALENCE; REAL-TIME POLYMERASE CHAIN REACTION; SAINT KITTS AND NEVIS;

EID: 84927173499     PISSN: 16617827     EISSN: 16604601     Source Type: Journal    
DOI: 10.3390/ijerph110807953     Document Type: Article

References (20)

1. Pappas, G.; Papdimitriou, P.; Siozopoulou, V.; Christou, L.; Akritidis, N. The globalization of leptospirosis: Worldwide incidence trends. Int. J. Infect. Dis. 2008, 12, 351-357.
2. Levett, P.N. Leptospirosis. Clin. Microbiol. Rev. 2001, 14, 296-326.
3. Ko, A.I.; Goarant, C.; Picardeau, M. Leptospira: The dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat. Rev. Microbiol. 2009, 7, 736-747.
4. Adler, B.; de laPena Moctezuma, A. Leptospira and leptospirosis. Vet. Microbiol. 2010, 140, 287-296.
5. Trueba, G.; Zapata, S.; Madrid, K.; Cullen, P.; Haake, D. Cell aggregation: A mechanism of pathogenic Leptospira to survive in fresh water. Int. Microbiol. 2004, 7, 35-40.
6. Fuortes, L.; Nettleman, M. Leptospirosis: A consequence of the Iowa flood. Iowa Med. 1994, 84, 449-450.
7. Sanders, E.J.; Rigau-Perez, J.G.; Smits, H.L.; Deseda, C.C.; Vorndam, V.A.; Aye, T.; Spiegel, R.A.; Weyant, R.S.; Bragg, S.L. Increase in leptospirosis in dengue-negative patients after a hurricane in Puerto Rico in 1996. Am. J. Trop. Med. Hyg. 1999, 61, 399-404.
8. Stoddard, R.A.; Gee, J.E.; Wilkins, P.P.; McCaustland, K.; Hoffmaster, A.R. Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene. Diagn. Microbiol. Infect. Dis. 2009, 64, 247-255.
9. Vein, J.; Perrin, A.; Berny, P.J.; Benoit, E.; Leblond, A.; Kodjo, A. Adaptation of a real-time PCR method for the detection and quantification of pathogenic leptospires in environmental water. Can. J. Microbiol. 2012, 58, 828-835.
10. Brenner, D.J.; McWhorter, A.C.; Knutson, J.K.; Steigerwalt, A.G. Escherichia vulneris: A new species of Enterobacteriaceae associated with human wounds. J. Clin. Microbiol. 1982, 15, 1133-1140.
11. Levett, P.N.; Morey, R.E.; Galloway, R.L.; Turner, D.E.; Steigerwalt, A.G.; Mayer, L.W. Detection of pathogenic leptospires by real-time quantitative PCR. J. Med. Microbiol. 2005, 54, 45-49.
12. Hochedez, P.; Escher, M.; Decoussy, H.; Pasgrimaud, L.; Martinez, R.; Rosine, J.; Théodose, R.; Bourhy, P.; Picardeau, M.; Olive, C.; et al. Outbreak of leptospirosis among canyoning participants, Martinique, 2011. Eurosurveillance 2013, 18, 20472:1-20472:8.
13. Stern, E.J.; Galloway, R.; Shadomy, S.V.; Wannemuehler, K.; Atrubin, D.; Blackmore, C.; Wofford, T.; Wilkins, P.P.; Ari, M.D.; Harris, L.; et al. Outbreak of leptospirosis among Adventure Race participants in Florida, 2005. Clin. Infect. Dis. 2010, 50, 843-849.
14. Narita, M.; Fujitani, S.; Haake, D.A.; Paterson, D.L. Leptospirosis after recreational exposure to water in the Yaeyama islands, Japan. Am. J. Trop. Med. Hyg. 2005, 73, 652-656.
15. Koay, T.K.; Nirmal, S.; Noitie, L.; Tan, E. An epidemiological investigation of an outbreak of leptospirosis associated with swimming, Beaufort, Sabah. Med. J. Malays. 2004, 59, 455-459.
16. Haddock, R.L.; Gilmore, J.W.; Pimentel, F. A leptospirosis outbreak on Guam associated with an athletic event. Pac. Health Dialog 2002, 9, 186-189.
17. Paul, J.H.; Jeffrey, W.H.; David, A.W.; DeFlaun, M.F.; Cazares, L.H. Turnover of extracellular DNA in eutrophic and oligotrophic freshwater environments of southwest Florida. Appl. Environ. Microbiol. 1989, 55, 1823-1828.
18. Everard, C.O.; Fraser-Chanpong, G.M.; Bhagwandin, L.J.; Race, M.W.; James, A.C. Leptospires in wildlife from Trinidad and Grenada. J. Wildl. Dis. 1983, 19, 192-199.
19. Adesiyun, A.A.; Baboolal, S.; Suepaul, S.; Dookeran, S.; Stewart-Johnson, A. Human leptospirosis in the Caribbean, 1997-2005: Characteristics and serotyping of clinical samples from 14 countries. Revista Panamericana de Salud Publica 2011, 29, 350-357.
20. Wood, H.; Drebot, M.A.; Dewailly, E.; Dillon, L.; Dimitrova, K.; Forde, M.; Grolla, A.; Lee, E.; Loftis, A.; Makowski, K.; et al. Seroprevalence of seven zoonotic pathogens in pregnant women from the Caribbean. Am. J. Trop. Med. Hyg. 2014, doi:10.4269/ajtmh.14-0107.