Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood

Journal of Applied Microbiology

Volumn 118, Issue 5, 2015, Pages 1199-1209

  Boyd, M A ^a,b,d   Tennant, S M ^a,b   Melendez, J H ^c   Toema, D ^a,b   Galen, J E ^a,b   Geddes, C D ^c   Levine, M M ^a,b  

^a UNIVERSITY OF MARYLAND   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

^d CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

Blood; Detection; Diagnostic; Invasive; PCR; Salmonella; Sensitive; Typhoid

Indexed keywords

BLOOD; CHLORINE COMPOUNDS; DEVELOPING COUNTRIES; DNA; EXTRACTION; POLYMERASE CHAIN REACTION;

BLOOD; DETECTION; DIAGNOSTIC; INVASIVE; LYSIS BUFFER; MICROWAVE-ACCELERATED METAL-ENHANCED FLUORESCENCES; PCR; REAL-TIME PCR; SENSITIVE; TYPHOID;

SALMONELLA;

AMMONIUM CHLORIDE; BLOOD CLOTTING FACTOR; BUFFER; DEXTRAN; WATER;

ADAPTATION; AMMONIUM COMPOUND; ASSAY; BACTERIUM; BLOOD; DETECTION METHOD; DEVELOPING WORLD; DNA; EXTRACTION METHOD; FRAGMENTATION; SENSITIVITY ANALYSIS; TYPHOID;

ADAPTATION; ARTICLE; ASSAY; BACTERIUM DETECTION; BACTERIUM ISOLATION; BLOOD ANALYSIS; BLOOD COLLECTION TUBE; BLOOD CULTURE; BLOOD STORAGE; DNA EXTRACTION; DNA FRAGMENTATION; ERYTHROCYTE; HEMOLYSIS; HUMAN; LEUKOCYTE; LYMPHOCYTE; MICROWAVE ACCELERATED METAL ENHANCED FLUORESCENCE ASSAY; MICROWAVE RADIATION; NONHUMAN; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHI; TYPHOID FEVER; ANALYSIS; BLOOD; CHEMISTRY; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; PROCEDURES; SALMONELLA ENTERICA SEROVAR TYPHI; SENSITIVITY AND SPECIFICITY;

SALMONELLA; SALMONELLA TYPHI;

ANALYTIC SAMPLE PREPARATION METHODS; ERYTHROCYTES; HUMANS; REAL-TIME POLYMERASE CHAIN REACTION; SALMONELLA TYPHI; SENSITIVITY AND SPECIFICITY; TYPHOID FEVER;

EID: 84926527653     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12769     Document Type: Article

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