Isolation and handling of mouse embryonic fibroblasts

Methods in Molecular Biology

Volumn 1194, Issue , 2014, Pages 247-252

  Jain, Kanika ^a   Verma, Paul J ^a,b   Liu, Jun ^a  

^a MONASH UNIVERSITY   (Australia)

^b SOUTH AUSTRALIAN RESEARCH AND DEVELOPMENT INSTITUTE   (Australia)

Author keywords

Cell culture; Feeder layers; Mitomycin C; Mouse embryonic fibroblasts

Indexed keywords

MITOMYCIN;

ANIMAL CELL; ARTICLE; CELL INACTIVATION; CELL ISOLATION; CELL PASSAGING; CLINICAL PROTOCOL; EMBRYO; FIBROBLAST; FREEZING; MOUSE; MOUSE EMBRYONIC FIBROBLAST; NONHUMAN; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS; THAWING; ANIMAL; CELL SEPARATION; CRYOPRESERVATION; CYTOLOGY; FEEDER CELL; FEMALE; PREGNANCY; PROCEDURES;

ANIMALS; CELL SEPARATION; CRYOPRESERVATION; FEEDER CELLS; FEMALE; FIBROBLASTS; MICE; PREGNANCY;

EID: 84925884847     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1215-5_13     Document Type: Article

References (10)

1. Nagy A, Gertsenstein M, Vintersten K, Behringer R. (2006) Preparing mouse embryo fi broblasts. CSH Protoc. doi: 10.1101/pdb. prot4398
2. Emichalska A. (2007) Isolation and propagation of mouse embryonic fi broblasts and preparation of mouse embryonic feeder layer cells. Curr Protoc Stem Cell Biol Chapter 1: Unit1C 3
3. Martin GR. (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci U S A 78:7634-7638
4. Evans MJ, Kaufman MH. (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292:154-156
5. Takahashi K, Yamanaka S. (2006) Induction of pluripotent stem cells from mouse embryonic and adult fi broblast cultures by defi ned factors. Cell 126:663-676
6. Okita K, Ichisaka T, Yamanaka S. (2007) Generation of germline-competent induced pluripotent stem cells. Nature 448:313-317
7. Nagy A, Gertsenstein M, Vintersten K, Behringer R. (2006) Preparing feeder cell layers from STO or mouse embryo fi broblast (MEF) cells: Treatment with mitomycin C. CSH Protoc. doi: 10.1101/pdb.prot4399
8. Nagy A, Gertsenstein M, Vintersten K, Behringer R. (2006) Preparing feeder cell layers from STO or mouse embryo fi broblast (MEF) cells: Treatment with gamma-irradiation. CSH Protoc. doi: 10.1101/pdb.prot4400
9. Robertson EJ. (1997) Derivation and maintenance of embryonic stem cell cultures. Methods Mol Biol 75:173-184
10. Wesselschmidt RL, Schwartz PH. (2011) The stem cell laboratory: Design, equipment, and oversight. Methods Mol Biol 767:3-13