LPS quantitation procedures

Methods in Molecular Biology

Volumn 1149, Issue , 2014, Pages 375-402

  Lam, Joseph S ^a   Anderson, Erin M ^a   Hao, Youai ^a  

^a NONE

Author keywords

Densitometry; Kdo; LAL assay; LPS; Phenol hot water method; Western blots

Indexed keywords

LIPOPOLYSACCHARIDE; SUGAR; PHENOL; POLYMYXIN B; WATER;

ANALYTIC METHOD; ARTICLE; BACTERIAL MEMBRANE; BACTERIAL STRAIN; BURKHOLDERIA; CONTROLLED STUDY; DENSITOMETRY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; WESTERN BLOTTING; WILD TYPE; AFFINITY CHROMATOGRAPHY; BIOASSAY; BIOCHEMISTRY; CHEMISTRY; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

BURKHOLDERIA; NEGIBACTERIA; PSEUDOMONAS AERUGINOSA;

BIOCHEMISTRY; BIOLOGICAL ASSAY; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; DENSITOMETRY; LIPOPOLYSACCHARIDES; PHENOL; POLYMYXIN B; PSEUDOMONAS AERUGINOSA; WATER;

EID: 84925884458     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0473-0_31     Document Type: Article

References (61)

1. Westphal O, Jann K (1965) Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. In: Whistler RL (ed) Methods in carbohydrate chemistry. Academic Press, New York, pp 83–91
2. Hickman J, Ashwell G (1966) Isolation of a bacterial lipopolysaccharide from Xanthomonas campestris containing 3-acetamido-3,6- dideoxy-D- galactose and D-rhamnose. J Biol Chem 241:1424–1428
3. Kasai N, Nowotny A (1967) Endotoxic glycolipid from a heptoseless mutant of Salmonella minnesota. J Bacteriol 94:1824–1836
4. Galanos C, Luderitz O, Westphal O (1969) A new method for the extraction of R lipopolysaccharides. Eur J Biochem 9:245–249
5. Darveau RP, Hancock RE (1983) Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J Bacteriol 155:831–838
6. Hitchcock PJ, Brown TM (1983) Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 154:269–277
7. Islam ST, Fieldhouse RJ, Anderson EM, Taylor VL, Keates RA, Ford RC, Lam JS (2012) A cationic lumen in the Wzx flippase mediates anionic O-antigen subunit translocation in Pseudomonas aeruginosa PAO 1. Mol Microbiol 84:1165–1176
8. Fomsgaard A, Freudenberg MA, Galanos C (1990) Modification of the silver staining technique to detect lipopolysaccharide in polyacrylamide gels. J Clin Microbiol 28: 2627–2631
9. Tsai CM, Frasch CE (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119:115–119
10. Hardy E, Pupo E, Castellanos-Serra L, Reyes J, Fernandez-Patron C (1997) Sensitive reverse staining of bacterial lipopolysaccharides on polyacrylamide gels by using zinc and imidazole salts. Anal Biochem 244:28–32
11. Kido N, Ohta M, Kato N (1990) Detection of lipopolysaccharides by ethidium bromide staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Bacteriol 172:1145–1147
12. Kittelberger R, Hilbink F (1993) Sensitive silver-staining detection of bacterial lipopolysaccharides in polyacrylamide gels. J Biochem Biophy Meth 26:81–86
13. Redwan EM (2012) Simple, sensitive, and quick protocol to detect less than 1 ng of bacterial lipopolysaccharide. Prep Biochem Biotech 42:171–182
14. Zhu ZX, Cong WT, Zhou X, Chen M, Choi JK, Jin LT, Li XK (2012) Negative staining of lipopolysaccharides on polyacrylamide gels by using eosin B. Anal Biochem 426:1–3
15. Hancock RE, Mutharia LM, Chan L, Darveau RP, Speert DP, Pier GB (1983) Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains. Infect Immun 42:170–177
16. Wang X, Zhang C, Shi F, Hu X (2010) Purification and characterization of lipopolysaccharides. Subcell Biochem 53:27–51
17. Forsberg LS, Carlson RW (1998) The structures of the lipopolysaccharides from Rhizobium etli strains CE358 and CE 359. The complete structure of the core region of R. etli lipopolysaccharides. J Biol Chem 273: 2747–2757
18. Ding JL, Navas MA 3rd, Ho B (1995) Molecular cloning and sequence analysis of factor C cDNA from the Singapore horseshoe crab, Carcinoscorpius rotundicauda. Mol Mar Biol Biotechnol 4:90–103
19. Lindsay GK, Roslansky PF, Novitsky TJ (1989) Single-step, chromogenic Limulus amebocyte lysate assay for endotoxin. J Clin Microbiol 27:947–951
20. Noda K, Goto H, Murakami Y, Ahmed AB, Kuroda A (2010) Endotoxin assay by bioluminescence using mutant firefly luciferase. Anal Biochem 397:152–155
21. Bang FB (1956) A bacterial disease of Limulus polyphemus. Bull Johns Hopkins Hosp 98:325–351
22. Iwanaga S, Miyata T, Tokunaga F, Muta T (1992) Molecular mechanism of hemolymph clotting system in Limulus. Throm Res 68:1–32
23. Williams KL (2001) LAL discovery, mechanism and application. In: Williams KL (ed) Endotoxins. Pyrogens, LAL testing, and depyrogenation, 2nd edn. Revised and expanded, Marcel Dekker Inc., New York, pp 178–203
24. Kawasaki H, Nose T, Muta T, Iwanaga S, Shimohigashi Y, Kawabata S (2000) Head-to-tail polymerization of coagulin, a clottable protein of the horseshoe crab. J Biol Chem 275:35297–35301
25. Teller JD, Kelly KM (1979) A turbidimetric Limulus amebocyte assay for the quantitative determination of Gram negative bacterial endotoxin. Prog Clin Biol Res 29:423–433
26. Unger FM (1981) The chemistry and biological significance of 3-deoxy-D-manno-2-octulosonic acid (KDO). Adv Carbohydr Chem Biochem 38:323–388
27. Brade H (1985) Occurrence of 2-keto-deoxyoctonic acid 5-phosphate in lipopolysaccharides of Vibrio cholerae Ogawa and Inaba. J Bacteriol 161:795–798
28. Hurlbert RE, Weckesser J, Mayer H, Fromme I (1976) Isolation and characterization of the lipopolysaccharide of Chromatium vinosum. Eur J Biochem 68:365–371
29. Weissbach A, Hurwitz J (1959) The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J Biol Chem 234:705–709
30. Osborn MJ (1963) Studies on the Gram-negative cell wall. I. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella Typhimurium. Proc Nat Acad Sci U S A 50:499–506
31. Warren L (1959) The thiobarbituric acid assay of sialic acids. J Biol Chem 234:1971–1975
32. Karkhanis YD, Zeltner JY, Jackson JJ, Carlo DJ (1978) A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipo- polysaccharide of Gram-negative bacteria. Anal Biochem 85:595–601
33. Skoza L, Mohos S (1976) Stable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de-O-acetylation. Biochem J 159:457–462
34. Lee CH, Tsai CM (1999) Quantification of bacterial lipopolysaccharides by the purpald assay: measuring formaldehyde generated from 2-keto-3-deoxyoctonate and heptose at the inner core by periodate oxidation. Anal Biochem 267:161–168
35. Nondek L, Rodler DR, Birks JW (1992) Measurement of sub-ppbv concentrations of aldehydes in a forest atmosphere using a new HPLC technique. Environ Sci Tech 26:1174–1178
36. Jacobson NW, Dickinson RG (1974) Spectrometric assay of aldehydes as 6-mercapto-3- substituted-S-triazolo(4,3-B)- S-tetrazines. Anal Chem 46:298–299
37. Guthrie RD (1962) Periodate oxidation. In: Whistler RL, Wolfrom ML (eds) Methods in carbohydrate chemistry. Academic Press, New York, pp 432–435
38. Speck JCJ (1962) Advances in carbohydrate chemistry. In: Whistler RL (ed) Methods in carbohydrate chemistry. Academic Press, New York, pp 441–445
39. Quesenberry MS, Lee YC (1996) A rapid formaldehyde assay using purpald reagent: application under periodation conditions. Anal Biochem 234:50–55
40. Burnette WN (1981) “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate–polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112:195–203
41. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Nat Acad Sci U S A 76:4350–4354
42. de Kievit TR, Lam JS (1994) Monoclonal antibodies that distinguish inner core, outer core, and lipid A regions of Pseudomonas aeruginosa lipopolysaccharide. J Bacteriol 176: 7129–7139
43. Lam JS, MacDonald LA, Lam MY (1987) Production of monoclonal antibodies against serotype strains of Pseudomonas aeruginosa. Infect Immun 55:2854–2856
44. Lam JS, MacDonald LA, Lam MY, Duchesne LG, Southam GG (1987) Production and characterization of monoclonal antibodies against serotype strains of Pseudomonas aeruginosa. Infect Immun 55:1051–1057
45. Lam MY, McGroarty EJ, Kropinski AM, MacDonald LA, Pedersen SS, Hoiby N, Lam JS (1989) Occurrence of a common lipopolysaccharide antigen in standard and clinical strains of Pseudomonas aeruginosa. J Clin Microbiol 27:962–967
46. Gassmann M, Grenacher B, Rohde B, Vogel J (2009) Quantifying Western blots: pitfalls of densitometry. Electrophoresis 30:1845–1855
47. Huszar G, Jenei B, Szabo G, Medgyesi GA (2002) Detection of pyrogens in intravenous IgG preparations. Biologicals 30:77–83
48. Ochiai M, Yamamoto A, Kataoka M, Toyoizumi H, Horiuchi Y (2001) Interfering effect of diphtheria-tetanus-acellular pertussis combined (DTaP) vaccines on the bacterial endotoxin test. Biologicals 29:55–58
49. Pfeiffer M, Weiss AR (1987) Removal of LAL-test interfering low molecular weight substances by ultrafiltration. Prog Clin Biol Res 231:251–262
50. Tsai CM, Frasch CE, Rivera E, Hochstein HD (1989) Measurements of lipopolysaccharide (endotoxin) in meningococcal protein and polysaccharide preparations for vaccine usage. J Biol Stand 17:249–258
51. Roslansky PF, Novitsky TJ (1991) Sensitivity of Limulus amebocyte lysate (LAL) to LAL-reactive glucans. J Clin Microbiol 29:2477–2483
52. Brade H, Galanos C, Luderitz O (1983) Differential determination of the 3-deoxy-D-mannooctulosonic acid residues in lipopolysaccharides of Salmonella minnesota rough mutants. Eur J Biochem 131:195–200
53. Greenberg ID, McNicholas P (1987) Positional changes in a hemangioma demonstrated by red blood cell imaging. Clin Nuc Med 12:668–670
54. Helander IM, Lindner B, Brade H, Altmann K, Lindberg AA, Rietschel ET, Zahringer U (1988) Chemical structure of the lipopolysaccharide of Haemophilus influenzae strain I-69 Rd-/b+. Description of a novel deep-rough chemotype. Eur J Biochem 177:483–492
55. Wu LH, Tsai CM, Frasch CE (1987) A method for purification of bacterial R-type lipopolysaccharides (lipooligosaccharides). Anal Biochem 160:281–289
56. Zamze SE, Ferguson MA, Moxon ER, Dwek RA, Rademacher TW (1987) Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide. Biochem J 245: 583–587
57. Carof M, Lebbar S, Szabo L (1987) Do endotoxins devoid of 3-deoxy-D-manno-2-octulosonic acid exist? Biochem Biophy Res Commun 143:845–847
58. Carof M, Lebbar S, Szabo L (1987) Detection of a 3-deoxy-2-octulosonic acid in thiobarbiturate- negative endotoxins. Carbohydr Res 161:C4–C7
59. Lesse AJ, Campagnari AA, Bittner WE, Apicella MA (1990) Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Immunol Meth 126:109–117
60. Wilcox RR (2003) Applying contemporary statistical techniques. Academic, San Diego, CA
61. Osaki T, Kawabata S (2004) Structure and function of coagulogen, a clottable protein in horseshoe crabs. Cell Mol Life Sci 61: 1257–1265