Quantitative fluorescence co-localization to study protein-receptor complexes

Methods in Molecular Biology

Volumn 1008, Issue , 2013, Pages 439-453

  Pompey, Shanica N ^a   Michaely, Peter ^a   Luby Phelps, Katherine ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Co localization; Confocal; Deconvolution; Digital imaging; Fluorescence; Immunofluorescence; LDL; LDL receptor; VLDL

Indexed keywords

COMPLEMENTARY DNA; EARLY ENDOSOME ANTIGEN 1; LIGAND; LOW DENSITY LIPOPROTEIN RECEPTOR; RECEPTOR; FLUORESCENT DYE; LOW DENSITY LIPOPROTEIN; VESICULAR TRANSPORT PROTEIN;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; IMMUNOFLUORESCENCE; LIGHT INTENSITY; MACROMOLECULE; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; QUANTITATIVE ANALYSIS; CELL LINE; CHEMISTRY; COMPUTER ASSISTED DIAGNOSIS; COMPUTER PROGRAM; CONFOCAL MICROSCOPY; HUMAN; MOLECULAR IMAGING;

CELL LINE; FLUORESCENT DYES; HUMANS; IMAGE INTERPRETATION, COMPUTER-ASSISTED; LIPOPROTEINS, LDL; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MOLECULAR IMAGING; RECEPTORS, LDL; SOFTWARE; VESICULAR TRANSPORT PROTEINS;

EID: 84925559066     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-398-5_16     Document Type: Article

References (12)

1. Manders EM, Stap J, Brakenhoff GJ, Van Driel R, Aten JA (1992) Dynamics of threedimensional replication patterns during the Sphase, analysed by double labelling of DNA and confocal microscopy. J Cell Sci 103(Pt3):857-862
2. Manders EMM, Verbeek FJ, Aten JA (1993) Measurement of co-localization of objects in dual-colour confocal images. J Microsc 169:375-382
3. Costes SV, Daelemans D, Cho EH, Dobbin Z, Pavlakis G, Lockett S (2004) Automatic and quantitative measurement of protein-protein colocalization in live cells. Biophys J 86:3993-4003
4. van Steensel B, van Binnendijk EP, Hornsby CD, Van Der Voort HT, Krozowski ZS, de Kloet ER, Van Driel R (1996) Partial colocalization of glucocorticoid and mineralocorticoid receptors in discrete compartments in nuclei of rat hippocampus neurons. J Cell Sci 109(Pt 4):787-792
5. Li Q, Lau A, Morris TJ, Guo L, Fordyce CB, Stanley EF (2004) A syntaxin 1, Galpha(o), and N-Type calciumchannel complex at a presynaptic nerve terminal: Analysis by quantitative immunocolocalization. J Neurosci 24:4070-4081
6. Bolte S, Cordelieres FP (2006) A guided tour into subcellular colocalization analysis in light microscopy. J Microsc 224:213-232
7. Comeau JW, Costantino S, Wiseman PW (2006) A guide to accurate fluorescence microscopy colocalization measurements. Biophys J 91:4611-4622
8. Adler J, Pagakis SN, Parmryd I (2008) Replicate-based noise corrected correlation for accurate measurements of colocalization. J Microsc 230:121-133
9. RamíRez O, GarcIá A, Rojas R, Couve AHA, Rtel S (2010) Confined displacement algorithm determines true and random colocalization in fluorescence microscopy. J Microsc 239:173-183
10. Toomre D, Bewersdorf J (2010) A new wave of cellular imaging. Annu Rev Cell Dev Biol 26:285-314
11. Zhao Z, Michaely P (2009) The role of calcium in lipoprotein release by the lowdensity lipoprotein receptor. Biochemistry 48:7313-7324
12. Image documentation for JaCoP can be found here: Http://imagejdocu.tudor. lu/lib/exe/fetch.php?mediaplugin:analysis:jacop-2.0: Just-another- colocalization-plugin:jacop-ij conf2008.pdf Documentation and a link to the source code for Coloc 2, Colocalization Threshold and Colocalization Test can be found here: Http://fiji.sc/wiki/Colocalization- Analysis