Improving fertilization rate in ICSI cycles by adding myoinositol to the semen preparation procedures: a prospective, bicentric, randomized trial on sibling oocytes

Journal of Assisted Reproduction and Genetics

Volumn 32, Issue 3, 2015, Pages 387-394

  Rubino, Patrizia ^a,d   Palini, Simone ^b   Chigioni, Sara ^a   Carlomagno, Gianfranco ^c   Quagliariello, Antonella ^a   De Stefani, Silvia ^b   Baglioni, Andrea ^a   Bulletti, Carlo ^b  

^a Tecnolab Casa di Cura La Madonnina   (Italy)

^b D Cervesi Hospital   (Italy)

^c LoLipharma srl dipartimento R D   (Italy)

^d SAN RAFFAELE HOSPITAL   (Italy)

Author keywords

Embryo quality; Fertilization rate; ICSI outcomes; Myoinositol

Indexed keywords

FOLLITROPIN; INOSITOL; PLACEBO; PROGESTERONE;

ADULT; ARTICLE; BLASTOCYST; CONTROLLED STUDY; DRUG EFFICACY; EMBRYO CULTURE; EMBRYO TRANSFER; ENDOMETRIAL THICKNESS; FEMALE; FERTILIZATION; FOLLITROPIN BLOOD LEVEL; HUMAN; IN VITRO STUDY; INTRACYTOPLASMIC SPERM INJECTION; MAJOR CLINICAL STUDY; MALE; NIDATION; OOCYTE; OUTCOME ASSESSMENT; PREGNANCY RATE; PRIORITY JOURNAL; PROGESTERONE BLOOD LEVEL; PROSPECTIVE STUDY; RANDOMIZED CONTROLLED TRIAL; SEMEN ANALYSIS; SIBLING; SPERM QUALITY; SPERMATOZOON; SPERMATOZOON DENSITY; SPERMATOZOON MOTILITY; CYTOLOGY; DRUG EFFECTS; IN VITRO FERTILIZATION; INFERTILITY, MALE; PREGNANCY; SPERM;

ADULT; EMBRYO TRANSFER; FEMALE; FERTILIZATION; FERTILIZATION IN VITRO; HUMANS; INFERTILITY, MALE; INOSITOL; MALE; OOCYTES; PREGNANCY; PREGNANCY RATE; SEMEN; SPERM INJECTIONS, INTRACYTOPLASMIC; SPERMATOZOA;

EID: 84925489100     PISSN: 10580468     EISSN: 15737330     Source Type: Journal    
DOI: 10.1007/s10815-014-0401-2     Document Type: Article

References (30)

1. Jimenez-Gonzalez C, Michelangeli F, Harper CV, Barratt CL, Publicover SJ. Calcium signalling in human spermatozoa: a specialized ‘toolkit’ of channels, transporters and stores. Hum Reprod Update. 2006;12(3):253–67.
2. Alasmari W, Barratt CL, Publicover SJ, Whalley KM, Foster E, Kay V, et al. The clinical significance of calcium-signalling pathways mediating human sperm hyperactivation. Hum Reprod. 2013;28(4):866–76.
3. Ho HC, Suarez SS. An inositol 1,4,5-trisphosphate receptor-gated intracellular Ca(2+) store is involved in regulating sperm hyperactivated motility. Biol Reprod. 2001;65(5):1606–15.
4. Ho HC, Suarez SS. Characterization of the intracellular calcium store at the base of the sperm flagellum that regulates hyperactivated motility. Biol Reprod. 2003;68(5):1590–6.
5. Harper CV, Barratt CL, Publicover SJ. Stimulation of human spermatozoa with progesterone gradients to simulate approach to the oocyte. Induction of [Ca(2+)](i) oscillations and cyclical transitions in flagellar beating. J Biol Chem. 2004;279(44):46315–25.
6. Costello S, Michelangeli F, Nash K, Lefievre L, Morris J, Machado-Oliveira G, et al. Ca2 + −stores in sperm: their identities and functions. Reproduction. 2009;138(3):425–37.
7. Lishko PV, Kirichok Y, Ren D, Navarro B, Chung JJ, Clapham DE. The control of male fertility by spermatozoan ion channels. Annu Rev Physiol. 2012;74:453–75.
8. Vermassen E, Parys JB, Mauger JP. Subcellular distribution of the inositol 1,4,5-trisphosphate receptors: functional relevance and molecular determinants. Biol Cell / Auspices Eur Cell Biol Organ. 2004;96(1):3–17.
9. Michelangeli F, Mezna M, Tovey S, Sayers LG. Pharmacological modulators of the inositol 1,4,5-trisphosphate receptor. Neuropharmacology. 1995;34(9):1111–22.
10. Kuroda Y, Kaneko S, Yoshimura Y, Nozawa S, Mikoshiba K. Are there inositol 1,4,5-triphosphate (IP3) receptors in human sperm? Life Sci. 1999;65(2):135–43.
11. Walensky LD, Snyder SH. Inositol 1,4,5-trisphosphate receptors selectively localized to the acrosomes of mammalian sperm. J Cell Biol. 1995;130(4):857–69.
12. Naaby-Hansen S, Wolkowicz MJ, Klotz K, Bush LA, Westbrook VA, Shibahara H, et al. Co-localization of the inositol 1,4,5-trisphosphate receptor and calreticulin in the equatorial segment and in membrane bounded vesicles in the cytoplasmic droplet of human spermatozoa. Mol Hum Reprod. 2001;7(10):923–33.
13. Berridge MJ. Inositol lipids and cell proliferation. Biochim Biophys Acta. 1987;907(1):33–45.
14. Carlomagno G, Nordio M, Chiu TT, Unfer V. Contribution of myo-inositol and melatonin to human reproduction. Eur J Obstet Gynecol Reprod Biol. 2011;159(2):267–72.
15. Condorelli RA, La Vignera S, Bellanca S, Vicari E, Calogero AE. Myoinositol: does it improve sperm mitochondrial function and sperm motility? Urology. 2012;79(6):1290–5.
16. Marchetti C, Obert G, Deffosez A, Formstecher P, Marchetti P. Study of mitochondrial membrane potential, reactive oxygen species, DNA fragmentation and cell viability by flow cytometry in human sperm. Hum Reprod. 2002;17(5):1257–65.
17. Marchetti C, Jouy N, Leroy-Martin B, Defossez A, Formstecher P, Marchetti P. Comparison of four fluorochromes for the detection of the inner mitochondrial membrane potential in human spermatozoa and their correlation with sperm motility. Hum Reprod. 2004;19(10):2267–76.
18. Marchetti P, Ballot C, Jouy N, Thomas P, Marchetti C. Influence of mitochondrial membrane potential of spermatozoa on in vitro fertilisation outcome. Andrologia. 2012;44(2):136–41.
19. Rienzi L, Ubaldi F, Iacobelli M, Ferrero S, Minasi MG, Martinez F, et al. Day 3 embryo transfer with combined evaluation at the pronuclear and cleavage stages compares favourably with day 5 blastocyst transfer. Hum Reprod. 2002;17(7):1852–5.
20. Gardner DK, Schoolcraft WB. Culture and transfer of human blastocysts. Curr Opin Obstet Gynecol. 1999;11(3):307–11.
21. Kvist U, Bjorndahl L, Manual on Basic Semen Analysis, ESHRE Monographs. Oxford: Oxford University Press; 2002. p. 2002.
22. Ramalho-Santos J. A sperm’s tail: the importance of getting it right. Hum Reprod. 2011;26(9):2590–1.
23. Nishimura Y, Yoshinari T, Naruse K, Yamada T, Sumi K, Mitani H, et al. Active digestion of sperm mitochondrial DNA in single living sperm revealed by optical tweezers. Proc Natl Acad Sci U S A. 2006;103(5):1382–7.
24. Li Z, Zhou Y, Liu R, Lin H, Liu W, Xiao W, et al. Effects of semen processing on the generation of reactive oxygen species and mitochondrial membrane potential of human spermatozoa. Andrologia. 2012;44(3):157–63.
25. O’Connell M, McClure N, Powell LA, Steele EK, Lewis SE. Differences in mitochondrial and nuclear DNA status of high-density and low-density sperm fractions after density centrifugation preparation. Fertil Steril. 2003;79 Suppl 1:754–62.
26. Sakkas D, Manicardi GC, Tomlinson M, Mandrioli M, Bizzaro D, Bianchi PG, et al. The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies. Hum Reprod. 2000;15(5):1112–6.
27. Wang X, Sharma RK, Gupta A, George V, Thomas AJ, Falcone T, et al. Alterations in mitochondria membrane potential and oxidative stress in infertile men: a prospective observational study. Fertil Steril. 2003;80 Suppl 2:844–50.
28. Lowther KM, Weitzman VN, Maier D, Mehlmann LM. Maturation, fertilization, and the structure and function of the endoplasmic reticulum in cryopreserved mouse oocytes. Biol Reprod. 2009;81(1):147–54.
29. Meseguer M, Santiso R, Garrido N, Garcia-Herrero S, Remohi J, Fernandez JL. Effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality. Fertil Steril. 2011;95(1):124–8.
30. Colazingari S, Fiorenza MT, Carlomagno G, Najjar R, Bevilacqua A. Improvement of mouse embryo quality by myo-inositol supplementation of IVF media. J Assist Reprod Genet. 2014;31(4):463–9.