Detection of OXA-48-type carbapenemase-producing Enterobacteriaceae in diagnostic laboratories can be enhanced by addition of bicarbonates to cultivation media or reaction buffers

Folia Microbiologica

Volumn 60, Issue 2, 2015, Pages 119-129

  Studentova, Vendula ^a   Papagiannitsis, Costas C ^a   Izdebski, Radoslaw ^b   Pfeifer, Yvonne ^c   Chudackova, Eva ^a   Bergerova, Tamara ^a   Gniadkowski, Marek ^b   Hrabak, Jaroslav ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

^b NATIONAL MEDICINES INSTITUTE   (Poland)

^c ROBERT KOCH INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BETA LACTAMASE; BICARBONATE; BUFFER; CARBAPENEMASE; CULTURE MEDIUM;

CHEMISTRY; CULTURE MEDIUM; ENTEROBACTERIACEAE; ENTEROBACTERIACEAE INFECTIONS; ENZYMOLOGY; EVALUATION STUDY; ISOLATION AND PURIFICATION; MATRIX-ASSISTED LASER DESORPTION-IONIZATION MASS SPECTROMETRY; MICROBIAL SENSITIVITY TEST; MICROBIOLOGY; PROCEDURES; SECRETION (PROCESS); SENSITIVITY AND SPECIFICITY;

BACTERIAL PROTEINS; BETA-LACTAMASES; BICARBONATES; BUFFERS; CULTURE MEDIA; ENTEROBACTERIACEAE; ENTEROBACTERIACEAE INFECTIONS; MICROBIAL SENSITIVITY TESTS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84925461581     PISSN: 00155632     EISSN: 18749356     Source Type: Journal    
DOI: 10.1007/s12223-014-0349-8     Document Type: Article

References (35)

1. Almarsson O, Kaufman MJ, Stong JD, Wu Y, Mayr SM, Petrich MA, Williams JM (1998) Meropenem exists in equilibrium with a carbon dioxide adduct in bicarbonate solution. J Pharm Sci 87:663–666
2. Antunes NT, Lamoureaux TL, Toth M, Stewart NK, Frase H, Vakulenko SB (2014) Class D β-lactamases: are they all carbapenemases? Antimicrob Agents Chemother 58:2119–2125
3. Barrett KE, Barman SM, Boitano S, Brooks H (2009) Ganong’s review of medical physiology, 23rd edn. McGraw-Hill Medical, NY
4. Burtis CA (2006) Tietz, textbook of clinical chemistry and molecular diagnostics, 4th edn. Carl A. Burtis et al, Elsevier, St. Louis, MO
5. Bush K, Jacoby GA, Medeiros AA (1995) A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother 39:1211–1233
6. Chudackova E, Bergerova T, Fajfrlik K, Cervena D, Urbaskova P, Empel J, Gniadkowski M, Hrabak J (2010) Carbapenem-non-susceptible strains of Klebsiella pneumoniae producing SHV-5 and/or DHA-1 β-lactamases in a Czech hospital. FEMS Microbiol Lett 309:62–70
7. Che T, Bethel CR, Pusztai-Carey M, Bonomo RA, Carey PR (2014) The different inhibition mechanisms of OXA-1 and OXA-24 β-lactamases are determined by the stability of active site carboxylated lysine. J Biol Chem 289:6152–6164
8. Docquier JD, Calderone V, De Luca F, Benvenuti M, Giuliani F, Bellucci L, Tafi A, Nordmann P, Botta M, Rossolini GM, Mangani S (2009) Crystal structure of the OXA-48 beta-lactamase reveals mechanistic diversity among class D carbapenemases. Chem Biol 16:540–547
9. Empel J, Hrabak J, Kozinska A, Bergerova T, Urbaskova P, Kern-Zdanowicz I, Gniadkowski M (2010) DHA-1-producing Klebsiella pneumoniae in a teaching hospital in the Czech Republic. Microbiol Drug Res 16:291–295
10. European Committee on Antimicrobial Susceptibility Testing (2003) Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilution. Clin Microbiol Infect 9:1–7
11. European Committee on Antimicrobial Susceptibility Testing (2013a) Breakpoint tables for interpretation of MICs and zone diameters, version 3.1. http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/Breakpoint_table_v_3.1.pdf. Accessed 15 September 2013
12. Fournet-Fayard S, Joly B, Forestier C (1995) Transformation of wild type Klebsiella pneumoniae with plasmid DNA by electroporation. J Microbiol Methods 24:49–54
13. Glasner C, Albiger B, Buist G, Tambić Andrasević A, Canton R, Carmeli Y, Friedrich A, Giske C, Glupczynski Y, Gniadkowski M, Livermore D, Nordmann P, Poirel L, Rossolini G, Seifert H, Vatopoulos A, Walsh T, Woodford N, Donker T, Monnet D, Grundmann H; European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) working group (2013) Carbapenemase-producing Enterobacteriaceae in Europe: a survey among national experts from 39 countries, February 2013. Euro Surveill 18:pii = 20525
14. Hrabák J, Chudackova E, Walkova R (2013) Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry for detection of antibiotic resistance mechanisms: from research to routine diagnosis. Clin Microbiol Rev 26:103–114
15. Hrabak J, Empel J, Bergerova T, Fajfrlik K, Urbaskova P, Kern-Zdanowicz I, Hryniewicz W, Gniadkowski M (2009) International clones of Klebsiella pneumoniae and Escherichia coli with extended-spectrum β-lactamases (ESBLs) in a Czech hospital. J Clin Microbiol 47:3353–3357
16. Hrabak J, Niemczyková J, Chudackova E, Fridrichova M, Studentova V, Cervena D, Urbaskova P, Zemlickova H (2011) KPC-2-producing Klebsiella pneumoniae isolated from a Czech patient previously hospitalized in Greece and in vivo selection of colistin resistance. Folia Microbiol 56:361–365
17. Hrabák J, Papagiannitsis CC, Chudáčková E (2014) Detection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories. Clin Microb Infect: in press. DOI: 10.1111/1469-0691.12678
18. Hrabak J, Papagiannitsis CC, Studentova V, Jakubu V, Fridrichova M, Zemlickova H and Czech Participants of European Antimicrobial Resistance Surveillance Network (2013) First outbreaks of carbapenemase-producing Klebsiella pneumoniae in the Czech Republic in 2011. Euro Surveill 18:pii = 20626
19. Hrabak J, Studentova V, Walkova R, Zemlickova H, Jakubu V, Chudackova E, Gniadkowski M, Pfeifer Y, Perry JD, Wilkinson K, Bergerova T (2012) Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 carbapenemases by MALDI-TOF mass spectrometry. J Clin Microbiol 50:2441–2443
20. June CM, Vallier BC, Bonomo RA, Leonard DA, Powersa RA (2014) Structural origins of oxacillinase specificity in class D β-lactamases. Antimicrob Agents Chemother 58:333–341
21. Laraki N, Franceschini N, Rossolini GM, Santucci P, Meunier C, de Pauw E, Amicosante G, Frère JM, Galleni M (1999) Biochemical characterization of the Pseudomonas aeruginosa 101/1477 metallo-beta-lactamase IMP-1 produced by Escherichia coli. Antimicrob Agents Chemother 43:902–906
22. Leonard DA, Hujer AM, Smith BA, Schneider KD, Bethel CR, Hujer KM, Bonomo RA (2008) The role of OXA-1 beta-lactamase Asp (66) in the stabilization of the active-site carbamate group and in substrate turnover. Biochem J 410:455–462
23. Livermore DM, Andrews JM, Hawkey PM, Ho PL, Keness Y, Doi Y, Paterson D, Woodford N (2012) Are susceptibility tests enough, or should laboratories still seek ESBLs and carbapenemases directly? J Antimicrob Chemother 67:1569–1577
24. Munoz-Price LS, Poirel L, Bonomo RA, Schwaber MJ, Daikos GL, Cormican M, Cornaglia G, Garau J, Gniadkowski M, Hayden MK, Kumarasamy K, Livermore DM, Maya JJ, Nordmann P, Patel JB, Paterson DL, Pitout J, Villegas MV, Wang H, Woodford N, Quinn JP (2013) Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases. Lancet Infect Dis 13:785–796
25. Nordmann P, Poirel L (2013) Strategies for identification of carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother 68:487–489
26. Papagiannitsis CC, Studentova V, Chudackova E, Bergerova T, Hrabak J, Radej J, Novak I (2013) Identification of a New Delhi metallo-β-lactamase-4 (NDM-4)-producing Enterobacter cloacae from a Czech patient previously hospitalized in Sri Lanka. Folia Microbiol 58:547–549
27. Pfeifer Y, Schlatterer K, Engelmann E, Schiller RA, Frangenberg HR, Stiewe D, Holfelder M, Witte W, Nordmann P, Poirel L (2012) Emergence of OXA-48-type carbapenemase-producing Enterobacteriaceae in German hospitals. Antimicrob Agent Chemother 56:2125–2128
28. Poirel L, Héritier C, Tolün V, Nordmann P (2004) Emergence of oxacillinase-mediated resistance to imipenem in Klebsiella pneumoniae. Antimicrob Agents Chemother 48:15–22
29. Poirel L, Potron A, Nordmann P (2012) OXA-48-like carbapenemases: the phantom menace. J Antimicrob Chemother 67:1597–1606
30. Sparbier K, Schubert S, Weller U, Boogen C, Kostrzewa M (2012) MALDI-TOF MS based functional assay for the rapid detection of resistance against β-lactam antibiotics. J Clin Microbiol 50:927–937
31. Tzouvelekis LS, Markogiannakis A, Psichogiou M, Tassios PT, Daikos GL (2012) Carbapenemases in Klebsiella pneumoniae and other Enterobacteriaceae: an evolving crisis of global dimensions. Clin Microbiol Rev 25:682–707
32. Vercheval L, Bauvois C, di Paolo A, Borel F, Ferrer JL, Sauvage E, Matagne A, Frère JM, Charlier P, Galleni M, Kerff F (2010) Three factors that modulate the activity of class D β-lactamases and interfere with the post-translational carboxylation of Lys70. Biochem J 432:495–504
33. Verma V, Testero SA, Amini K, Wei W, Liu J, Balachandran N, Monoharan T, Stynes S, Kotra LP, Golemi-Kotra D (2011) Hydrolytic mechanism of OXA-58 enzyme, a carbapenem-hydrolyzing class D β-lactamase from Acinetobacter baumannii. J Biol Chem 286:37292–37303
34. Wiskirchen DE, Nordmann P, Crandon JL, Nicolau DP (2014a) Efficacity of humanized carbapenem and ceftazidime regimens against Enterobacteriaceae producing OXA-48 carbapenemase in a murine infection model. Antimicrob Agents Chemother 58:1678–1683
35. Wiskirchen DE, Nordmann P, Crandon JL, Nicolau DP (2014b) In vivo efficacy of human simulated regimens of carbapenems and comparator agents against NDM-1 producing Enterobacteriaceae. Antimicrob Agents Chemother 58:1671–1677