Determining the specificities of TALENs, cas9, and other genome-editing enzymes

Methods in Enzymology

Volumn 546, Issue C, 2014, Pages 47-78

  Pattanayak, Vikram ^a   Guilinger, John P ^b,c   Liu, David R ^b,c  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD UNIVERSITY   (United States)

^c HARVARD UNIVERSITY   (United States)

Author keywords

Cas9; In vitro selection; Nuclease specificity; Specificity engineering; Specificity profiling; TALEN; ZFN

Indexed keywords

CRISPR ASSOCIATED PROTEIN; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; ENDONUCLEASE;

ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; DNA BINDING; DNA CLEAVAGE; DNA END JOINING REPAIR; DNA FLANKING REGION; DNA MODIFICATION; ENZYME SPECIFICITY; GENOME; HIGH THROUGHPUT SEQUENCING; HUMAN; IN VITRO SELECTION; INDEL MUTATION; MOLECULAR LIBRARY; NONHUMAN; PRIORITY JOURNAL; ZINC FINGER MOTIF; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; CRISPR CAS SYSTEM; GENETIC ENGINEERING; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES;

MAMMALIA;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; DNA CLEAVAGE; ENDONUCLEASES; GENETIC ENGINEERING; GENOME; HUMANS; MOLECULAR SEQUENCE DATA;

EID: 84925441397     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801185-0.00003-9     Document Type: Chapter

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