Imaging genomic elements in living cells using CRISPR/Cas9

Methods in Enzymology

Volumn 546, Issue C, 2014, Pages 337-354

  Chen, Baohui ^a   Huang, Bo ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Chromatin; CRISPR; Fluorescence; Microscopy; Nucleus; Telomere

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GENOMIC DNA; GUIDE RNA; LENTIVIRUS VECTOR; MUCIN 4; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; CRISPR ASSOCIATED PROTEIN; GREEN FLUORESCENT PROTEIN;

ARTICLE; BASE PAIRING; BINDING AFFINITY; CLONAL CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DNA STRAND; EPIFLUORESCENCE MICROSCOPY; EXPRESSION VECTOR; FEMALE; FLUORESCENCE IMAGING; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; GENE LOCUS; GENE TARGETING; HIGH THROUGHPUT SEQUENCING; HUMAN; HUMAN CELL; LENTIVIRUS INFECTION; NUCLEAR LOCALIZATION SIGNAL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA SEQUENCE; SENSITIVITY AND SPECIFICITY; CELL LINE; CRISPR CAS SYSTEM; GENE VECTOR; GENETICS; GENOMICS; HUMAN GENOME; LENTIVIRINAE; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES; TELOMERE; ULTRASTRUCTURE;

BASE SEQUENCE; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; GENETIC LOCI; GENETIC VECTORS; GENOME, HUMAN; GENOMICS; GREEN FLUORESCENT PROTEINS; HUMANS; LENTIVIRUS; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; TELOMERE;

EID: 84925437922     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801185-0.00016-7     Document Type: Chapter

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