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Volumn 1169, Issue , 2014, Pages 133-142

Expression profiling by real-time quantitative polymerase chain reaction (RT-qPCR)

Author keywords

Polymerase chain reaction; Primer design; Quantification; RT PCR

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; RNA; RNA DIRECTED DNA POLYMERASE;

EID: 84925226719     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0882-0_13     Document Type: Article
Times cited : (12)

References (11)
  • 1
    • 0028859959 scopus 로고
    • Quantitative RNA-polymerase chain reaction-DNA analysis by capillary electrophoresis and laser-induced fluorescence
    • Fasco MJ et al (1995) Quantitative RNA-polymerase chain reaction-DNA analysis by capillary electrophoresis and laser-induced fluorescence. Anal Biochem 224(1):140-147
    • (1995) Anal Biochem , vol.224 , Issue.1 , pp. 140-147
    • Fasco, M.J.1
  • 2
    • 0029985713 scopus 로고    scopus 로고
    • Quantitative competitive polymerase chain reaction: Analysis of amplified products of the HIV-1 gag gene by capillary electrophoresis with laser-induced fluorescence detection
    • Williams SJ et al (1996) Quantitative competitive polymerase chain reaction: analysis of amplified products of the HIV-1 gag gene by capillary electrophoresis with laser-induced fluorescence detection. Anal Biochem 236(1): 146-152
    • (1996) Anal Biochem , vol.236 , Issue.1 , pp. 146-152
    • Williams, S.J.1
  • 3
    • 0028069547 scopus 로고
    • Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma: Application to acute retroviral infection
    • Mulder J et al (1994) Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma: application to acute retroviral infection. J Clin Microbiol 32(2):292-300
    • (1994) J Clin Microbiol , vol.32 , Issue.2 , pp. 292-300
    • Mulder, J.1
  • 4
    • 0029957181 scopus 로고    scopus 로고
    • Real time quantitative PCR
    • Heid CA et al (1996) Real time quantitative PCR. Genome Res 6(10):986-994
    • (1996) Genome Res , vol.6 , Issue.10 , pp. 986-994
    • Heid, C.A.1
  • 5
    • 0032805947 scopus 로고    scopus 로고
    • A quantitative fluorescence-based microplate assay for the determination of double-stranded DNA using SYBR Green I and a standard ultraviolet transilluminator gel imaging system
    • Vitzthum F et al (1999) A quantitative fluorescence-based microplate assay for the determination of double-stranded DNA using SYBR Green I and a standard ultraviolet transilluminator gel imaging system. Anal Biochem 276(1):59-64
    • (1999) Anal Biochem , vol.276 , Issue.1 , pp. 59-64
    • Vitzthum, F.1
  • 6
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25(2):169-193
    • (2000) J Mol Endocrinol , vol.25 , Issue.2 , pp. 169-193
    • Bustin, S.A.1
  • 7
    • 0032883806 scopus 로고    scopus 로고
    • Housekeeping genes as internal standards: Use and limits
    • Thellin O et al (1999) Housekeeping genes as internal standards: use and limits. J Biotechnol 75(2-3):291-295
    • (1999) J Biotechnol , vol.75 , Issue.2-3 , pp. 291-295
    • Thellin, O.1
  • 8
    • 77953106571 scopus 로고    scopus 로고
    • Primer design for RT-PCR
    • Li K, Brownley A (2010) Primer design for RT-PCR. Methods Mol Biol 630:271-299
    • (2010) Methods Mol Biol , vol.630 , pp. 271-299
    • Li, K.1    Brownley, A.2
  • 9
    • 1542356381 scopus 로고    scopus 로고
    • Standardized determination of real-time PCR efficiency from a single reaction set-up
    • Tichopad A et al (2003) Standardized determination of real-time PCR efficiency from a single reaction set-up. Nucleic Acids Res 31(20):e122
    • (2003) Nucleic Acids Res , vol.31 , Issue.20 , pp. 122
    • Tichopad, A.1
  • 10
    • 0035710746 scopus 로고    scopus 로고
    • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method
    • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25(4):402-408
    • (2001) Methods , vol.25 , Issue.4 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2
  • 11
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time RT-PCR
    • PfafflMW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9):e45
    • (2001) Nucleic Acids Res , vol.29 , Issue.9 , pp. 45
    • Pfaffl, M.W.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.