Impact of metagenomic DNA extraction procedures on the identifiable endophytic bacterial diversity in Sorghum bicolor (L. Moench)

Journal of Microbiological Methods

Volumn 112, Issue , 2015, Pages 104-117

  Maropola, Mapula Kgomotso Annah ^a   Ramond, Jean Baptiste ^a,b   Trindade, Marla ^a  

^a UNIVERSITY OF THE WESTERN CAPE   (South Africa)

^b UNIVERSITY OF PRETORIA   (South Africa)

Author keywords

Endophytic bacteria; Metagenomic DNA extraction; Pyrosequencing; Sorghum root and stem; T RFLP

Indexed keywords

CETRIMIDE; DNA; DODECYL SULFATE SODIUM; METAGENOMIC DNA; RNA 16S; UNCLASSIFIED DRUG; BACTERIAL DNA; RIBOSOME DNA;

AGROBACTERIUM; ARTICLE; BACTERIA; BACTERIUM CULTURE; COMMUNITY STRUCTURE; CONTROLLED STUDY; DNA EXTRACTION; ENDOPHYTE; ENDOPHYTIC BACTERIUM; ERWINIA; HERBASPIRILLUM; INTERMETHOD COMPARISON; METAGENOMICS; MICROBACTERIUM; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; MICROBIOME; NONHUMAN; PLANT GROWTH; PLANT ROOT; PLANT STEM; PLANT TISSUE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS; PYROSEQUENCING; QUALITY CONTROL; RELIABILITY; REPRODUCIBILITY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SORGHUM; SPHINGOBACTERIUM; STENOTROPHOMONAS; BACTERIUM; BIODIVERSITY; CHEMISTRY; CLASSIFICATION; COMPARATIVE STUDY; DNA SEQUENCE; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; PROCEDURES; RNA GENE;

AGROBACTERIUM; BACTERIA (MICROORGANISMS); ERWINIA; HERBASPIRILLUM; MICROBACTERIUM; PSEUDOMONAS; SORGHUM BICOLOR; SPHINGOBACTERIUM; STENOTROPHOMONAS;

BACTERIA; BIODIVERSITY; DNA; DNA, BACTERIAL; DNA, RIBOSOMAL; ENDOPHYTES; GENES, RRNA; METAGENOMICS; PLANT ROOTS; PLANT STEMS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SORGHUM;

EID: 84925106285     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2015.03.012     Document Type: Article

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