Molecular profiling of the invasive tumor microenvironment in a 3-dimensional model of colorectal cancer cells and ex vivo fibroblasts

Journal of Visualized Experiments

Volumn , Issue 86, 2014, Pages

  Bullock, Marc D ^a   Mellone, Max ^a   Pickard, Karen M ^a   Sayan, Abdulkadir Emre ^a   Mitter, Richard ^b   Primrose, John N ^a   Packham, Graham K ^a   Thomas, Gareth ^a   Mirnezami, Alexander H ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

Author keywords

Cancer metastasis; Colorectal cancer; Epithelium; Fibroblasts; Invasion; Issue 86; Laser microdissection; Medicine; Molecular profiling; Organotypic culture; Stromal tissue; Tumor microenvironment

Indexed keywords

EID: 84924896732     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51475     Document Type: Article

References (10)

1. Nystrom, M.L., Thomas, G.J., Stone, M., Mackenzie, I.C., Hart, I.R., Marshall, J.F. Development of a quantitative method to analyse tumour cell invasion in organotypic culture. J Pathol. 205 (4), 468-475, (2005).
2. Zhang, L., et al. miR-153 supports colorectal cancer progression via pleiotropic effects that enhance invasion and chemotherapeutic resistance. Cancer Res. (2013).
3. Bullock, M.D., et al. Pleiotropic actions of miR-21 highlight the critical role of deregulated stromal microRNAs during colorectal cancer progression. Cell Death Dis. 20 (4), (2013).
4. Jenei, V., Nystrom, M.L., Thomas, G.J. Measuring invasion in an organotypic model. Methods Mol Biol. 769, 223-232, (2011).
5. De Wever, O., Demetter, P., Mareel, M., Bracke, M. Stromal myofibroblasts are drivers of invasive cancer growth. Int J Cancer. 123 (10), 2229-2238, (2008).
6. Fries, K.M., et al. Evidence of fibroblast heterogeneity and the role of fibroblast subpopulations in fibrosis. Clin Immunol Immunopathol. 72 (3), 283-292, (1994).
7. Moutasim, K.A., et al. Betel-derived alkaloid up-regulates keratinocyte alphavbeta6 integrin expression and promotes oral submucous fibrosis. J Pathol. 223 (3), 366-377, (2011).
8. Winter, J., Jung, S., Keller, S., Gregory, R.I., Diederichs S. Many roads to maturity: microRNA biogenesis pathways and their regulation. Nat Cell Biol. 11 (3), 228-234, (2009).
9. Li, J., et al. Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells. BMC Biotechnol. 7 (36), doi:10.1186/1472-6750-7-36 (2007).
10. Szafranska, A.E., et al. Accurate molecular characterization of formalin-fixed, paraffin-embedded tissues by microRNA expression profiling. J Mol Diagn. 10 (5), 415-423. (2008).