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Volumn 14, Issue 3, 2015, Pages

Rhizoglomus melanum, a new arbuscular mycorrhizal fungal species associated with submerged plants in freshwater lake Avsjøen in Norway

Author keywords

Aquatic; Arbuscular mycorrhiza; Biodiversity; Glomerales; Habitats; Phylogeny

Indexed keywords

FUNGI; GLOMERACEAE; GLOMERALES; GLOMEROMYCOTA; ISOETIDAE; LITTORELLA UNIFLORA; ZEA MAYS;

EID: 84924874052     PISSN: 1617416X     EISSN: 18618952     Source Type: Journal    
DOI: 10.1007/s11557-015-1031-5     Document Type: Article
Times cited : (27)

References (48)
  • 2
    • 78651456534 scopus 로고    scopus 로고
    • Molecular analysis of AMF diversity in aquatic macrophytes: a comparison of oligotrophic and utra-oligotrophic lakes
    • COI: 1:CAS:528:DC%2BC3MXmsVGitg%3D%3D
    • Baar J, Paradi I, Lucassen E, Hudson-Edwards KA, Redecker D, Roelofs JGM, Smolders AJP (2011) Molecular analysis of AMF diversity in aquatic macrophytes: a comparison of oligotrophic and utra-oligotrophic lakes. Aquat Bot 94:53–61. doi:10.1016/j.aquabot.2010.09.006
    • (2011) Aquat Bot , vol.94 , pp. 53-61
    • Baar, J.1    Paradi, I.2    Lucassen, E.3    Hudson-Edwards, K.A.4    Redecker, D.5    Roelofs, J.G.M.6    Smolders, A.J.P.7
  • 3
    • 0034843722 scopus 로고    scopus 로고
    • Occurrence of vesicular-arbuscular mycorrhiza in aquatic macrophytes from lakes and streams
    • Beck-Nielsen D, Madsen TV (2001) Occurrence of vesicular-arbuscular mycorrhiza in aquatic macrophytes from lakes and streams. Aquat Bot 71:141–148. doi:10.1016/s0304-3770(01)00180-2
    • (2001) Aquat Bot , vol.71 , pp. 141-148
    • Beck-Nielsen, D.1    Madsen, T.V.2
  • 4
    • 84884206521 scopus 로고    scopus 로고
    • W. Szafer Institute of Botany, Polish Academyof Sciences, Kraków:
    • Błaszkowski J (2012) Glomeromycota. W. Szafer Institute of Botany, Polish Academyof Sciences, Kraków
    • (2012) Glomeromycota
    • Błaszkowski, J.1
  • 5
    • 84915785691 scopus 로고    scopus 로고
    • Rhizophagus natalensis, a new species in the Glomeromycota
    • Błaszkowski J, Chwat G, Góralska A, Goto BT (2014) Rhizophagus natalensis, a new species in the Glomeromycota. Mycotaxon 129:97–108. doi:10.5248/129.97
    • (2014) Mycotaxon , vol.129 , pp. 97-108
    • Błaszkowski, J.1    Chwat, G.2    Góralska, A.3    Goto, B.T.4
  • 6
    • 84922422843 scopus 로고    scopus 로고
    • Two new genera, Dominikia and Kamienska, and D. disticha sp. nov. in Glomeromycota
    • Błaszkowski J, Chwat G, Góralska A, Ryska P, Kovács GM (2015) Two new genera, Dominikia and Kamienska, and D. disticha sp. nov. in Glomeromycota. Nova Hedwigia 100:225–238. doi:10.1127/nova_hedwigia/2014/0216
    • (2015) Nova Hedwigia , vol.100 , pp. 225-238
    • Błaszkowski, J.1    Chwat, G.2    Góralska, A.3    Ryska, P.4    Kovács, G.M.5
  • 8
    • 63549106576 scopus 로고    scopus 로고
    • Occurrence and coexistence of arbuscular mycorrhizal fungi and dark septate fungi in aquatic macrophytes in a tropical river-floodplain system
    • de Marins JF, Carrenho R, Thomaz SM (2009) Occurrence and coexistence of arbuscular mycorrhizal fungi and dark septate fungi in aquatic macrophytes in a tropical river-floodplain system. Aquat Bot 91:13–19. doi:10.1016/j.aquabot.2009.01.001
    • (2009) Aquat Bot , vol.91 , pp. 13-19
    • de Marins, J.F.1    Carrenho, R.2    Thomaz, S.M.3
  • 9
    • 0022244970 scopus 로고
    • The occurrence of vesicular-arbuscular mycorrhiza in isoetid-type submerged aquatic macrophytes under naturally varying conditions
    • Farmer AM (1985) The occurrence of vesicular-arbuscular mycorrhiza in isoetid-type submerged aquatic macrophytes under naturally varying conditions. Aquat Bot 21:245–249. doi:10.1016/0304-3770(85)90052-x
    • (1985) Aquat Bot , vol.21 , pp. 245-249
    • Farmer, A.M.1
  • 10
    • 3142637959 scopus 로고
    • Endogone spores in cultivated soils
    • Gerdemann JW, Nicolson TH (1962) Endogone spores in cultivated soils. Nature 195:308–309. doi:10.1038/195308b0
    • (1962) Nature , vol.195 , pp. 308-309
    • Gerdemann, J.W.1    Nicolson, T.H.2
  • 11
    • 74549125386 scopus 로고    scopus 로고
    • Seaview version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building
    • COI: 1:CAS:528:DC%2BC3cXntlGgtw%3D%3D, PID: 19854763
    • Gouy M, Guindon S, Gascuel O (2010) Seaview version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 27:221–224
    • (2010) Mol Biol Evol , vol.27 , pp. 221-224
    • Gouy, M.1    Guindon, S.2    Gascuel, O.3
  • 12
    • 84986992409 scopus 로고
    • The effect of magnesium ions on the vesicular-arbuscular mycorrhizal infection of maize roots
    • COI: 1:CAS:528:DyaK3sXitlaqtbg%3D
    • Gryndler M, Vejsadová H, Vančura V (1992) The effect of magnesium ions on the vesicular-arbuscular mycorrhizal infection of maize roots. New Phytol 122:455–460. doi:10.1111/j.1469-8137.1992.tb00073.x
    • (1992) New Phytol , vol.122 , pp. 455-460
    • Gryndler, M.1    Vejsadová, H.2    Vančura, V.3
  • 13
    • 77950806408 scopus 로고    scopus 로고
    • New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0
    • COI: 1:CAS:528:DC%2BC3cXks1Kms7s%3D, PID: 20525638
    • Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307–321
    • (2010) Syst Biol , vol.59 , pp. 307-321
    • Guindon, S.1    Dufayard, J.F.2    Lefort, V.3    Anisimova, M.4    Hordijk, W.5    Gascuel, O.6
  • 14
    • 0000135962 scopus 로고
    • Species and mycorrhizal infections of New Zealand Endogonaceae
    • Hall IR (1977) Species and mycorrhizal infections of New Zealand Endogonaceae. Trans Br Mycol Soc 68:341–356. doi:10.1016/S0007-1536(77)80186-1
    • (1977) Trans Br Mycol Soc , vol.68 , pp. 341-356
    • Hall, I.R.1
  • 15
    • 33644761749 scopus 로고    scopus 로고
    • Occurrence of arbuscular mycorrhizas and dark septate endophytes in hydrophytes from lakes and streams in southwest China
    • Kai W, Zhao ZW (2006) Occurrence of arbuscular mycorrhizas and dark septate endophytes in hydrophytes from lakes and streams in southwest China. Int Rev Hydrobiol 91:29–37. doi:10.1002/iroh.200510827
    • (2006) Int Rev Hydrobiol , vol.91 , pp. 29-37
    • Kai, W.1    Zhao, Z.W.2
  • 16
    • 84875619226 scopus 로고    scopus 로고
    • MAFFT multiple sequence alignment software version 7: improvements in performance and usability
    • COI: 1:CAS:528:DC%2BC3sXksFWisLc%3D, PID: 23329690
    • Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780. doi:10.1093/molbev/mst010
    • (2013) Mol Biol Evol , vol.30 , pp. 772-780
    • Katoh, K.1    Standley, D.M.2
  • 18
    • 84879228334 scopus 로고
    • A modified procedure for staining roots to detect VA mycorrhizas
    • Koske RE, Gemma JN (1989) A modified procedure for staining roots to detect VA mycorrhizas. Mycol Res 92:486–505
    • (1989) Mycol Res , vol.92 , pp. 486-505
    • Koske, R.E.1    Gemma, J.N.2
  • 19
    • 59549087639 scopus 로고
    • Scutellospora hawaiiensis, a new arbuscular mycorrhizal fungus from Hawaii
    • Koske RE, Gemma JN (1995) Scutellospora hawaiiensis, a new arbuscular mycorrhizal fungus from Hawaii. Mycologia 76:250–255
    • (1995) Mycologia , vol.76 , pp. 250-255
    • Koske, R.E.1    Gemma, J.N.2
  • 20
    • 0002183257 scopus 로고
    • A convenient, permanent slide mounting medium
    • Koske RE, Tessier B (1983) A convenient, permanent slide mounting medium. Mycol Soc Am Newsl 34:59
    • (1983) Mycol Soc Am Newsl , vol.34 , pp. 59
    • Koske, R.E.1    Tessier, B.2
  • 21
    • 66649137511 scopus 로고    scopus 로고
    • DNA-based species level detection of Glomeromycota: one PCR primer set for all arbuscular mycorrhizal fungi
    • PID: 19368665
    • Krüger M, Stockinger H, Krüger C, Schüßler A (2009) DNA-based species level detection of Glomeromycota: one PCR primer set for all arbuscular mycorrhizal fungi. New Phytol 183:212–223. doi:10.1111/j.1469-8137.2009.02835.x
    • (2009) New Phytol , vol.183 , pp. 212-223
    • Krüger, M.1    Stockinger, H.2    Krüger, C.3    Schüßler, A.4
  • 22
    • 84856534511 scopus 로고    scopus 로고
    • Phylogenetic reference data for systematics and phylotaxonomy of arbuscular mycorrhizal fungi from phylum to species level
    • PID: 22150759
    • Krüger M, Krüger C, Walker C, Stockinger H, Schüßler A (2012) Phylogenetic reference data for systematics and phylotaxonomy of arbuscular mycorrhizal fungi from phylum to species level. New Phytol 193:970–984. doi:10.1111/j.1469-8137.2011.03962.x
    • (2012) New Phytol , vol.193 , pp. 970-984
    • Krüger, M.1    Krüger, C.2    Walker, C.3    Stockinger, H.4    Schüßler, A.5
  • 23
    • 84904410351 scopus 로고    scopus 로고
    • Molecular diversity of arbuscular mycorrhizal fungi associated with two co-occurring perennial plant species on a Tibetan altitudinal gradient
    • PID: 23912811
    • Li X, Gai J, Li X, Christie P, Zhang F, Zhang J (2014) Molecular diversity of arbuscular mycorrhizal fungi associated with two co-occurring perennial plant species on a Tibetan altitudinal gradient. Mycorrhiza 24(2):95–107. doi:10.1007/s00572-013-0518-7
    • (2014) Mycorrhiza , vol.24 , Issue.2 , pp. 95-107
    • Li, X.1    Gai, J.2    Li, X.3    Christie, P.4    Zhang, F.5    Zhang, J.6
  • 24
    • 0021560138 scopus 로고
    • Mehlich 3 soil test extractant: a modification of Mehlich 2 extractant
    • COI: 1:CAS:528:DyaL2MXhvVChsro%3D
    • Mehlich A (1984) Mehlich 3 soil test extractant: a modification of Mehlich 2 extractant. Commun Soil Sci Plant Anal 15:1409–1416
    • (1984) Commun Soil Sci Plant Anal , vol.15 , pp. 1409-1416
    • Mehlich, A.1
  • 25
    • 3142729569 scopus 로고    scopus 로고
    • Colonisation and molecular diversity of arbuscular mycorrhizal fungi in the aquatic plants Littorella uniflora and Lobelia dortmanna in southern Sweden
    • COI: 1:CAS:528:DC%2BD2cXlt1Sjtrw%3D, PID: 15323243
    • Nielsen KB, Kjøller R, Olsson PA, Schweiger PF, Andersen FO, Rosendahl S (2004) Colonisation and molecular diversity of arbuscular mycorrhizal fungi in the aquatic plants Littorella uniflora and Lobelia dortmanna in southern Sweden. Mycol Res 108:616–625. doi:10.1017/s0953756204000073
    • (2004) Mycol Res , vol.108 , pp. 616-625
    • Nielsen, K.B.1    Kjøller, R.2    Olsson, P.A.3    Schweiger, P.F.4    Andersen, F.O.5    Rosendahl, S.6
  • 26
    • 17444420355 scopus 로고    scopus 로고
    • Glomus badium, a new sporocarpic arbuscular mycorrhizal fungal species from European grasslands of higher soil pH
    • Oehl F, Redecker D, Sieverding E (2005) Glomus badium, a new sporocarpic arbuscular mycorrhizal fungal species from European grasslands of higher soil pH. J Appl Bot Food Qual 79:38–43
    • (2005) J Appl Bot Food Qual , vol.79 , pp. 38-43
    • Oehl, F.1    Redecker, D.2    Sieverding, E.3
  • 28
    • 80052918635 scopus 로고    scopus 로고
    • Glomeromycota: three new genera, and glomoid species reorganized
    • Oehl F, Silva GA, Goto BT, Sieverding E (2011b) Glomeromycota: three new genera, and glomoid species reorganized. Mycotaxon 116:75–120. doi:10.5248/116.75
    • (2011) Mycotaxon , vol.116 , pp. 75-120
    • Oehl, F.1    Silva, G.A.2    Goto, B.T.3    Sieverding, E.4
  • 29
    • 84937718862 scopus 로고    scopus 로고
    • Dominikia bernensis, a new arbuscular mycorrhizal fungus from a Swiss no-till farming site, and D. aurea, D. compressa, and D. indica, three new combinations in Dominikia. Nova Hedwigia 101, available online
    • Oehl F, Sánchez-Castro I, Sousa NMF, Silva GA, Palenzuela J (2015) Dominikia bernensis, a new arbuscular mycorrhizal fungus from a Swiss no-till farming site, and D. aurea, D. compressa, and D. indica, three new combinations in Dominikia. Nova Hedwigia 101, available online. doi:10.1127/nova_hedwigia/2014/0235
    • (2015)
    • Oehl, F.1    Sánchez-Castro, I.2    Sousa, N.M.F.3    Silva, G.A.4    Palenzuela, J.5
  • 30
    • 84906940984 scopus 로고    scopus 로고
    • Where the wild things are: looking for uncultured glomeromycota
    • Ohsowski BM, Zaitsoff PD, Öpik M, Hart MM (2014) Where the wild things are: looking for uncultured glomeromycota. New Phytol 204:170–179. doi:10.1111/nph.12894
    • (2014) New Phytol , vol.204 , pp. 170-179
    • Ohsowski, B.M.1    Zaitsoff, P.D.2    Öpik, M.3    Hart, M.M.4
  • 32
    • 84890578683 scopus 로고    scopus 로고
    • DNA-based detection and identification of Glomeromycota: the virtual taxonomy of environmental sequences
    • Öpik M, Davison J, Moora M, Zobel M (2014) DNA-based detection and identification of Glomeromycota: the virtual taxonomy of environmental sequences. Botany 92:135–147. doi:10.1139/cjb-2013-0110
    • (2014) Botany , vol.92 , pp. 135-147
    • Öpik, M.1    Davison, J.2    Moora, M.3    Zobel, M.4
  • 33
    • 79955962958 scopus 로고    scopus 로고
    • Ambispora granatensis, a new arbuscular mycorrhizal fungus, associated with Asparagus officinalis in Andalucía (Spain)
    • PID: 20952800
    • Palenzuela J, Barea JM, Ferrol N, Oehl F (2011) Ambispora granatensis, a new arbuscular mycorrhizal fungus, associated with Asparagus officinalis in Andalucía (Spain). Mycologia 103:333–340. doi:10.3852/09-146
    • (2011) Mycologia , vol.103 , pp. 333-340
    • Palenzuela, J.1    Barea, J.M.2    Ferrol, N.3    Oehl, F.4
  • 34
    • 84885946685 scopus 로고    scopus 로고
    • Acaulospora pustulata and Acaulospora tortuosa, two new species in the Glomeromycota associated with endangered plants in Sierra Nevada (southern Spain)
    • Palenzuela J, Azcón-Aguilar C, Barea JM, Silva GA, Oehl F (2013) Acaulospora pustulata and Acaulospora tortuosa, two new species in the Glomeromycota associated with endangered plants in Sierra Nevada (southern Spain). Nova Hedwigia 97:305–319. doi:10.1127/0029-5035/2013/0129
    • (2013) Nova Hedwigia , vol.97 , pp. 305-319
    • Palenzuela, J.1    Azcón-Aguilar, C.2    Barea, J.M.3    Silva, G.A.4    Oehl, F.5
  • 36
    • 0041386108 scopus 로고    scopus 로고
    • MrBayes 3: Bayesian phylogenetic inference under mixed models
    • COI: 1:CAS:528:DC%2BD3sXntlKms7k%3D, PID: 12912839
    • Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. doi:10.1093/bioinformatics/btg180
    • (2003) Bioinformatics , vol.19 , pp. 1572-1574
    • Ronquist, F.1    Huelsenbeck, J.P.2
  • 37
    • 84924866908 scopus 로고    scopus 로고
    • Arbuscular mycorrhizal fungi and their ecological roles: a review with a Norwegian perspective
    • Ruissen MA (2013a) Arbuscular mycorrhizal fungi and their ecological roles: a review with a Norwegian perspective. Agarica 33:105–116
    • (2013) Agarica , vol.33 , pp. 105-116
    • Ruissen, M.A.1
  • 38
    • 84924862646 scopus 로고    scopus 로고
    • Diversity of arbuscular mycorrhizal fungi in Norwegian agriculture: a pilot study
    • Ruissen MA (2013b) Diversity of arbuscular mycorrhizal fungi in Norwegian agriculture: a pilot study. Agarica 33:117–123
    • (2013) Agarica , vol.33 , pp. 117-123
    • Ruissen, M.A.1
  • 39
    • 84922281947 scopus 로고    scopus 로고
    • Rhizoglomus, a new genus in the Glomeraceae
    • Sieverding E, Silva GA, Berndt R, Oehl F (2014) Rhizoglomus, a new genus in the Glomeraceae. Mycotaxon 129:373–386. doi:10.5248/129.373
    • (2014) Mycotaxon , vol.129 , pp. 373-386
    • Sieverding, E.1    Silva, G.A.2    Berndt, R.3    Oehl, F.4
  • 40
    • 34848903620 scopus 로고    scopus 로고
    • Phylogenetic analysis of Glomeromycota by partial LSU rDNA sequences
    • COI: 1:CAS:528:DC%2BD28Xms1ers7w%3D, PID: 16534621
    • Silva GA, Lumini E, Maia LC, Bonfante P, Bianciotto V (2006) Phylogenetic analysis of Glomeromycota by partial LSU rDNA sequences. Mycorrhiza 16:183–189. doi:10.1007/s00572-005-0030-9
    • (2006) Mycorrhiza , vol.16 , pp. 183-189
    • Silva, G.A.1    Lumini, E.2    Maia, L.C.3    Bonfante, P.4    Bianciotto, V.5
  • 41
    • 0036063769 scopus 로고    scopus 로고
    • The isoetid environment: biogeochemistry and threats
    • COI: 1:CAS:528:DC%2BD38XltFSgtb4%3D
    • Smolders AJP, Lucassen E, Roelofs JGM (2002) The isoetid environment: biogeochemistry and threats. Aquat Bot 73:325–350. doi:10.1016/s0304-3770(02)00029-3
    • (2002) Aquat Bot , vol.73 , pp. 325-350
    • Smolders, A.J.P.1    Lucassen, E.2    Roelofs, J.G.M.3
  • 42
    • 0001248349 scopus 로고
    • Vesicular-arbuscular mycorrhiza in some aquatic vascular plants
    • Søndergaard M, Laegaard S (1977) Vesicular-arbuscular mycorrhiza in some aquatic vascular plants. Nature 268:232–233. doi:10.1038/268232a0
    • (1977) Nature , vol.268 , pp. 232-233
    • Søndergaard, M.1    Laegaard, S.2
  • 43
    • 0039037838 scopus 로고
    • Arguments for diagnoses based on unaltered wall structures
    • Spain JL (1990) Arguments for diagnoses based on unaltered wall structures. Mycotaxon 38:71–76
    • (1990) Mycotaxon , vol.38 , pp. 71-76
    • Spain, J.L.1
  • 44
    • 84901369153 scopus 로고    scopus 로고
    • Three new species of arbuscular mycorrhizal fungi discovered at one location in a desert of Oman: Diversispora omaniana, Septoglomus nakheelum and Rhizophagus arabicus
    • COI: 1:CAS:528:DC%2BC2cXpvFShsrc%3D, PID: 24782493
    • Symanczik S, Błaszkowski J, Chwat G, Boller T, Wiemken A, Al-Yahya’ei M (2014) Three new species of arbuscular mycorrhizal fungi discovered at one location in a desert of Oman: Diversispora omaniana, Septoglomus nakheelum and Rhizophagus arabicus. Mycologia 106:243–259. doi:10.3852/106.2.243
    • (2014) Mycologia , vol.106 , pp. 243-259
    • Symanczik, S.1    Błaszkowski, J.2    Chwat, G.3    Boller, T.4    Wiemken, A.5    Al-Yahya’ei, M.6
  • 45
    • 79957613599 scopus 로고    scopus 로고
    • MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods
    • COI: 1:CAS:528:DC%2BC3MXht1eiu73K, PID: 21546353
    • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739. doi:10.1093/molbev/msr121
    • (2011) Mol Biol Evol , vol.28 , pp. 2731-2739
    • Tamura, K.1    Peterson, D.2    Peterson, N.3    Stecher, G.4    Nei, M.5    Kumar, S.6
  • 46
    • 77951023403 scopus 로고    scopus 로고
    • Efficacy of indigenous arbuscular mycorrhizal fungi for promoting white yam (Dioscorea rotundata) growth in West Africa
    • Tchabi A, Coyne D, Hountondji F, Lawouin L, Wiemken A, Oehl F (2010) Efficacy of indigenous arbuscular mycorrhizal fungi for promoting white yam (Dioscorea rotundata) growth in West Africa. Appl Soil Ecol 45:92–100. doi:10.1016/j.apsoil.2010.03.001
    • (2010) Appl Soil Ecol , vol.45 , pp. 92-100
    • Tchabi, A.1    Coyne, D.2    Hountondji, F.3    Lawouin, L.4    Wiemken, A.5    Oehl, F.6
  • 48
    • 84884608618 scopus 로고    scopus 로고
    • The arbuscular mycorrhizal fungal community response to warming and grazing differs between soil and roots on the Qinghai-Tibetan Plateau
    • COI: 1:CAS:528:DC%2BC3sXhsFOntLjN, PID: 24086741
    • Yang W, Zheng Y, Gao C, He XH, Ding Q, Kim Y, Rui YC, Wang SP, Guo LD (2013) The arbuscular mycorrhizal fungal community response to warming and grazing differs between soil and roots on the Qinghai-Tibetan Plateau. PLoS ONE 8:e76447. doi:10.1371/journal.pone.0076447
    • (2013) PLoS ONE , vol.8 , pp. 76447
    • Yang, W.1    Zheng, Y.2    Gao, C.3    He, X.H.4    Ding, Q.5    Kim, Y.6    Rui, Y.C.7    Wang, S.P.8    Guo, L.D.9


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