Preparation of proper immunogen by cloning and stable expression of cDNA coding for human hematopoietic stem cell marker CD34 in NIH-3T3 mouse fibroblast cell line

Advanced Pharmaceutical Bulletin

Volumn 5, Issue 1, 2015, Pages 69-75

  Shafaghat, Farzaneh ^a   Abbasi Kenarsari, Hajar ^b   Majidi, Jafar ^a,b   Movassaghpour, Ali Akbar ^c   Shanehbandi, Dariush ^a   Kazemi, Tohid ^d  

^a TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^c TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^d TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

CD34; Cloning; Eukaryotic expression; HSCs; KG1a

Indexed keywords

AMPICILLIN; ANTIGEN; CD34 ANTIBODY; COMPLEMENTARY DNA;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ARTICLE; CELL COUNTING; CELL ISOLATION; FIBROBLAST; FLOW CYTOMETRY; GENE AMPLIFICATION; HEMATOPOIETIC STEM CELL; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN FUNCTION; TEMPERATURE; ULTRAVIOLET SPECTROPHOTOMETRY;

EID: 84924608638     PISSN: 22285881     EISSN: 22517308     Source Type: Journal    
DOI: 10.5681/apb.2015.009     Document Type: Article

References (35)

1. Drenou B. Flow cytometry for CD34 determination in hematopoietic grafts. Hematol Cell Ther 1996;38(6):505-12.
2. Greaves MF, Brown J, Molgaard HV, Spurr NK, Robertson D, Delia D, et al. Molecular features of CD34: a hemopoietic progenitor cell-associated molecule. Leukemia 1992;6 Suppl 1:31-6.
3. Pisacane Pisacane AM, Picciotto F, Risio M. CD31 and CD34 expression as immunohistochemical markers of endothelial transdifferentiation in human cutaneous melanoma. Cell Oncol 2007;29(1):59-66.
4. Simmons Simmons DL, Satterthwaite AB, Tenen DG, Seed B. Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells. J Immunol 1992;148(1):267-71.
5. Beauchamp JR, Heslop L, Yu DS, Tajbakhsh S, Kelly RG, Wernig A, et al. Expression of CD34 and Myf5 defines the majority of quiescent adult skeletal muscle satellite cells. J Cell Biol 2000;151(6):1221-34.
6. Nakamura Y, Komano H, Nakauchi H. Two alternative forms of cDNA encoding CD34. Exp Hematol 1993;21(2):236-42.
7. Hemmerich S, Butcher EC, Rosen SD. Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J Exp Med 1994;180(6):2219-26.
8. Kim YC, Wu Q, Chen J, Xuan Z, Jung YC, Zhang MQ, et al. The transcriptome of human CD34+ hematopoietic stem-progenitor cells. Proc Natl Acad Sci U S A 2009;106(20):8278-83.
9. Kishimoto S, Ishihara M, Kanatani Y, Nambu M, Takikawa M, Sumi Y, et al. Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines. J Tissue Eng 2011;2(1):2041731411425419.
10. Nielsen JS, Mcnagny KM. Novel functions of the CD34 family. J Cell Sci 2008;121(Pt 22):3683-92.
11. Blanchet MR, Maltby S, Haddon DJ, Merkens H, Zbytnuik L, Mcnagny KM. CD34 facilitates the development of allergic asthma. Blood 2007;110(6):2005-12.
12. Hernandez Mir G, Helin J, Skarp KP, Cummings RD, Makitie A, Renkonen R, et al. Glycoforms of human endothelial CD34 that bind L-selectin carry sulfated sialyl Lewis x capped O- and N-glycans. Blood 2009;114(3):733-41.
13. Gaipa G, Coustan-Smith E, Todisco E, Maglia O, Biondi A, Campana D. Characterization of CD34+, CD13+, CD33- cells, a rare subset of immature human hematopoietic cells. Haematologica 2002;87(4):347-56.
14. Testa JE, Chrastina A, Oh P, Li Y, Witkiewicz H, Czarny M, et al. Immunotargeting and cloning of two CD34 variants exhibiting restricted expression in adult rat endothelia in vivo. Am J Physiol Lung Cell Mol Physiol 2009;297(2):L251-62.
15. Wisniewski D, Affer M, Willshire J, Clarkson B. Further phenotypic characterization of the primitive lineage- CD34+CD38-CD90+CD45RA- hematopoietic stem cell/progenitor cell sub-population isolated from cord blood, mobilized peripheral blood and patients with chronic myelogenous leukemia. Blood Cancer J 2011;1(9):e36.
16. Colmegna I, Weyand CM. Haematopoietic stem and progenitor cells in rheumatoid arthritis. Rheumatology (Oxford) 2011;50(2):252-60.
17. Kelley TW, Huntsman D, Mcnagny KM, Roskelley CD, Hsi ED. Podocalyxin: a marker of blasts in acute leukemia. Am J Clin Pathol 2005;124(1):134-42.
18. Pusztaszeri MP, Seelentag W, Bosman FT. Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and Fli-1 in normal human tissues. J Histochem Cytochem 2006;54(4):385-95.
19. Mackie AR, Losordo DW. CD34-positive stem cells: in the treatment of heart and vascular disease in human beings. Tex Heart Inst J 2011;38(5):474-85.
20. Apiratmateekul N, Phunpae P, Kasinrerk W. A modified hybridoma technique for production of monoclonal antibodies having desired isotypes. Cytotechnology 2009.
21. Hollingsworth MA, Rebellato LM, Moore JW, Finn OJ, Metzgar RS. Antigens expressed on NIH 3T3 cells following transformation with DNA from human pancreatic tumor. Cancer Res 1986;46(5):2482-7.
22. Kazemi T, Tahmasebi F, Bayat AA, Mohajer N, Khoshnoodi J, Jeddi-Tehrani M, et al. Characterization of novel murine monoclonal antibodies directed against the extracellular domain of human HER2 tyrosine kinase receptor. Hybridoma (Larchmt) 2011;30(4):347-53.
23. Klapper LN, Vaisman N, Hurwitz E, Pinkas-Kramarski R, Yarden Y, Sela M. A subclass of tumor-inhibitory monoclonal antibodies to ErbB-2/HER2 blocks crosstalk with growth factor receptors. Oncogene 1997;14(17):2099-109.
24. Buhring HJ, Sures I, Jallal B, Weiss FU, Busch FW, Ludwig WD, et al. The receptor tyrosine kinase p185HER2 is expressed on a subset of B-lymphoid blasts from patients with acute lymphoblastic leukemia and chronic myelogenous leukemia. Blood 1995;86(5):1916-23.
25. Moscatelli D, Quarto N. Transformation of NIH 3T3 cells with basic fibroblast growth factor or the hst/K-fgf oncogene causes downregulation of the fibroblast growth factor receptor: reversal of morphological transformation and restoration of receptor number by suramin. J Cell Biol 1989;109(5):2519-27.
26. Nakayasu M, Shima H, Aonuma S, Nakagama H, Nagao M, Sugimura T. Deletion of transfected oncogenes from NIH 3T3 transformants by inhibitors of poly(ADP-ribose) polymerase. Proc Natl Acad Sci U S A 1988;85(23):9066-70.
27. Nigro S, Geido E, Infusini E, Orecchia R, Giaretti W. Transfection of human mutated K-ras in mouse NIH-3T3 cells is associated with increased cloning efficiency and DNA aneuploidization. Int J Cancer 1996;67(6):871-5.
28. Thorgeirsson UP, Turpeenniemi-Hujanen T, Williams JE, Westin EH, Heilman CA, Talmadge JE, et al. NIH/3T3 cells transfected with human tumor DNA containing activated ras oncogenes express the metastatic phenotype in nude mice. Mol Cell Biol 1985;5(1):259-62.
29. Kozak M. The scanning model for translation: an update. J Cell Biol 1989;108(2):229-41.
30. Mao M, Fu G, Wu JS, Zhang QH, Zhou J, Kan LX, et al. Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning. Proc Natl Acad Sci U S A 1998;95(14):8175-80.
31. Miraglia S, Godfrey W, Yin AH, Atkins K, Warnke R, Holden JT, et al. A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning. Blood 1997;90(12):5013-21.
32. Brown MC, Joaquim TR, Chambers R, Onisk DV, Yin F, Moriango JM, et al. Impact of immunization technology and assay application on antibody performance--a systematic comparative evaluation. PLoS One 2011;6(12):e28718.
33. IACUC Policy on Administering Complete Freund's Adjuvant (CFA) and other Adjuvants. [20 June 2014]; Available from: http://www.iacuc.emory.edu/documents/367_Complete_Freunds_Adjuvent.pdf.
34. Furley AJ, Reeves BR, Mizutani S, Altass LJ, Watt SM, Jacob MC, et al. Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines. Blood 1986;68(5):1101-7.
35. Hong HY, Sun YX, Guo YX, Wang JN, Lai CN, Qi ZT, et al. Cloning and Expression of Human CD20 Gene on NIH-3T3 Cell Membrane. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2000;32(4):430-3.