Microsatellite instable and microsatellite stable primary endometrial carcinoma cells and their subcutaneous and orthotopic xenografts recapitulate the characteristics of the corresponding primary tumor

International Journal of Gynecological Cancer

Volumn 25, Issue 3, 2015, Pages 363-371

  Schrauwen, Stefanie ^a   Coenegrachts, Lieve ^a   Depreeuw, Jeroen ^a,b,c   Luyten, Catherina ^a   Verbist, Godelieve ^a   Debruyne, David ^d   Vergote, Ignace ^a   Lambrechts, Diether ^b,c   Amant, Frédéric ^a  

^a UNIVERSITY HOSPITALS LEUVEN   (Belgium)

^b UNIVERSITY OF LEUVEN   (Belgium)

^c DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^d AZ GROENINGE   (Belgium)

Author keywords

Endometrial cancer; MSI; Orthotopic mouse model; Primary cell cultures

Indexed keywords

TELOMERASE; ESTROGEN RECEPTOR; KERATIN; MICROSATELLITE DNA; PROGESTERONE RECEPTOR; VIMENTIN;

ADULT; AGED; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CANCER PATIENT; CARCINOMA CELL; CELL CULTURE; CLINICAL FEATURE; DISTANT METASTASIS; ENDOMETRIUM CARCINOMA; ENZYME ACTIVITY; FEMALE; GENETIC TRAIT; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; IN VIVO STUDY; MICROSATELLITE INSTABILITY; MIDDLE AGED; MOUSE; NONHUMAN; ORTHOTOPIC TRANSPLANTATION; PRIMARY CELL CULTURE; PRIMARY TUMOR; PRIORITY JOURNAL; SUBCUTANEOUS XENOGRAFT; TUMOR CELL CULTURE; TUMOR XENOGRAFT; VALIDATION STUDY; ANIMAL; CANCER TRANSPLANTATION; CARCINOMA; CELL PROLIFERATION; CHEMISTRY; DISEASE MODEL; ENDOMETRIUM TUMOR; GENETICS; NUDE MOUSE; PATHOLOGY; PROCEDURES; SECONDARY;

ADULT; AGED; ANIMALS; CARCINOMA; CELL PROLIFERATION; DISEASE MODELS, ANIMAL; ENDOMETRIAL NEOPLASMS; FEMALE; HUMANS; KERATINS; MICE; MICE, NUDE; MICROSATELLITE INSTABILITY; MICROSATELLITE REPEATS; MIDDLE AGED; NEOPLASM TRANSPLANTATION; PRIMARY CELL CULTURE; RECEPTORS, ESTROGEN; RECEPTORS, PROGESTERONE; TUMOR CELLS, CULTURED; VIMENTIN;

EID: 84924417861     PISSN: 1048891X     EISSN: 15251438     Source Type: Journal    
DOI: 10.1097/IGC.0000000000000363     Document Type: Article

References (29)

1. Colombo N, Preti E, Landoni F, et al. Endometrial cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2013;24:vi33-vi38.
2. Jemal A, Bray F, Center MM, et al. Global cancer statistics. CA Cancer J Clin. 2011;61:69-90.
3. Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013. CA Cancer J Clin. 2013;63:11-30.
4. Colombo N, Preti E, Landoni F, et al. Endometrial cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2011;22:vi35-vi39.
5. Dinkelspiel HE, Wright JD, Lewin SN, et al. Contemporary clinical management of endometrial cancer. Obstet Gynecol Int. 2013;2013:583891.
6. Wright JD, Barrena Medel NI, Sehouli J, et al. Contemporary management of endometrial cancer. Lancet. 2012;379:1352-1360.
7. Bradford LS, Rauh-Hain JA, Schorge J, et al. Advances in the management of recurrent endometrial cancer. Am J Clin Oncol. June 11, 2013. doi: 10.1097/COC.0b013e31829a2974.
8. Domcke S, Sinha R, Levine DA, et al. Evaluating cell lines as tumour models by comparison of genomic profiles. Nat Commun. 2013;4:2126.
9. Maletzki C, Stier S, Gruenert U, et al. Establishment, characterization and chemosensitivity of three mismatch repair deficient cell lines from sporadic and inherited colorectal carcinomas. PLoS One. 2012;7:e52485.
10. McDermott U, Sharma SV, Dowell L, et al. Identification of genotype-correlated sensitivity to selective kinase inhibitors by using high-throughput tumor cell line profiling. Proc Natl Acad Sci U S A. 2007;104:19936-19941.
11. Onoda N, Nakamura M, Aomatsu N, et al. Establishment, characterization and comparison of seven authentic anaplastic thyroid cancer cell lines retaining clinical features of the original tumors. World J Surg. 2014;38:688-695.
12. Sos ML, Michel K, Zander T, et al. Predicting drug susceptibility of nonYsmall cell lung cancers based on genetic lesions. J Clin Invest. 2009;119:1727-1740.
13. Seol HS, Suh YA, Ryu YJ, et al. A patient-derived xenograft mouse model generated from primary cultured cells recapitulates patient tumors phenotypically and genetically. J Cancer Res Clin Oncol. 2013;139:1471-1480.
14. Doll A, Gonzalez M, Abal M, et al. An orthotopic endometrial cancer mouse model demonstrates a role for RUNX1 in distant metastasis. Int J Cancer. 2009;125:257-263.
15. Cabrera S, Llaurado M, Castellvi J, et al. Generation and characterization of orthotopic murine models for endometrial cancer. Clin Exp Metastasis. 2012;29:217-227.
16. Ibrahimi A, Vande VG, Reumers V, et al. Highly efficient multicistronic lentiviral vectors with peptide 2A sequences. Hum Gene Ther. 2009;20:845-860.
17. Garcia-Dios DA, Lambrechts D, Coenegrachts L, et al. High-throughput interrogation of PIK3CA, PTEN, KRAS, FBXW7 and TP53 mutations in primary endometrial carcinoma. Gynecol Oncol. 2013;128:327-334.
18. Zhao H, Thienpont B, Yesilyurt BT, et al. Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaks. Elife. 2014;3:e02725.
19. Korch C, Spillman MA, Jackson TA, et al. DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination. Gynecol Oncol. 2012;127:241-248.
20. Capes-Davis A, Theodosopoulos G, Atkin I, et al. Check your cultures! A list of cross-contaminated or misidentified cell lines. Int J Cancer. 2010;127:1-8.
21. Schweppe RE, Klopper JP, Korch C, et al. Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification. J Clin Endocrinol Metab. 2008;93:4331-4341.
22. RL OD, McCormick A, Mukhopadhyay A, et al. The use of ovarian cancer cells from patients undergoing surgery to generate primary cultures capable of undergoing functional analysis. PLoS One. 2014;9:e90604.
23. Dunfield LD, Shepherd TG, NachtigalMW. Primary culture and mRNA analysis of human ovarian cells. Biol Proced Online. 2002;4:55-61.
24. Mukhopadhyay A, Elattar A, Cerbinskaite A, et al. Development of a functional assay for homologous recombination status in primary cultures of epithelial ovarian tumor and correlation with sensitivity to poly(ADP-ribose) polymerase inhibitors. Clin Cancer Res. 2010;16:2344-2351.
25. Hunt RC, Davis AA. Altered expression of keratin and vimentin in human retinal pigment epithelial cells in vivo and in vitro. J Cell Physiol. 1990;145:187-199.
26. Hecht JL, Mutter GL. Molecular and pathologic aspects of endometrial carcinogenesis. J Clin Oncol. 2006;24:4783-4791.
27. Karamurzin Y, Rutgers JK. DNA mismatch repair deficiency in endometrial carcinoma. Int J Gynecol Pathol. 2009;28:239-255.
28. Tibiletti MG, Furlan D, Taborelli M, et al. Microsatellite instability in endometrial cancer: relation to histological subtypes. Gynecol Oncol. 1999;73:247-252.
29. Kandoth C, Schultz N, Cherniack AD, et al. Integrated genomic characterization of endometrial carcinoma. Nature.