Comparison of the GeneXpert Enterovirus assay (GXEA) with real-time one step RT-PCR for the detection of enteroviral RNA in the cerebrospinal fluid of patients with meningitis

Virology Journal

Volumn 12, Issue 1, 2015, Pages

  Hong, Jiyoung ^a,c   Kim, Ahyoun ^a   Hwang, Seoyeon ^a   Cheon, Doo Sung ^a   Kim, Jong Hyen ^b   Lee, June Woo ^a   Park, Jae Hak ^c   Kang, Byunghak ^a  

^a Korea Centers for Disease Control and Prevention   (South Korea)

^b The Catholic University of Korea College of Medicine   (South Korea)

^c Seoul National University   (South Korea)

Author keywords

Cerebrospinal fluid; Enteroviruses; GeneXpert Enterovirus Assay; Real time one step reverse transcription polymerase chain reaction

Indexed keywords

VIRUS RNA;

ARTICLE; ASEPTIC MENINGITIS; CEREBROSPINAL FLUID; CONTROLLED STUDY; COXSACKIE VIRUS; COXSACKIE VIRUS A24; COXSACKIE VIRUS B2; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; ECHO VIRUS 30; ENTEROVIRUS; ENTEROVIRUS 71; ENTEROVIRUS B; ENTEROVIRUS INFECTION; GENEXPERT ENTEROVIRUS ASSAY; HUMAN; HUMAN POLIOVIRUS 1; INTERMETHOD COMPARISON; LABORATORY DIAGNOSIS; LIMIT OF DETECTION; MOLECULAR DIAGNOSIS; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; SEROTYPE; VIRUS DETECTION; VIRUS LOAD; VIRUS STRAIN; COMPARATIVE STUDY; ENTEROVIRUS INFECTIONS; GENETICS; ISOLATION AND PURIFICATION; MENINGITIS; PROCEDURES; VIROLOGY;

ENTEROVIRUS;

ENTEROVIRUS; ENTEROVIRUS INFECTIONS; HUMANS; MENINGITIS; MOLECULAR DIAGNOSTIC TECHNIQUES; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL;

EID: 84924218643     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/s12985-015-0258-8     Document Type: Article

References (15)

1. Abzug MJ. Presentation, diagnosis, and management of enterovirus infections in neonates. Paediatr Drugs. 2004;6:1-10.
2. Ginocchio CC, Zhang F, Malhotra A, Manji R, Sillekens P, Foolen H, et al. Development, technical performance, and clinical evaluation of a NucliSens basic kit application for detection of enterovirus RNA in cerebrospinal fluid. J Clin Microbiol. 2005;43:2612-23.
3. King RL, Lorch SA, Cohen DM, Hodinka RL, Cohn KA, Shah SS. Routine cerebrospinal fluid enterovirus polymerase chain reaction testing reduces hospitalization and antibiotic use for infants 90 days of age or younger. Pediatrics. 2007;120:489-96.
4. Nolts FS. Case studies in cost effectiveness of molecular diagnostic for infectious diseases: pulmonary tuberculosis, enteroviral meningitis, and BK virus nephropathy. Clin Infect Dis. 2006;43:1463-7.
5. Parasuraman TV, Frenia K, Remero J. Enteroviral meningitis: cost of illness and considerations for the economic evaluation of potential therapies. Pharmacoeconomics. 2001;19:3-12.
6. Ramers C, Billman G, Hartin M, Ho S, Sawyer MH. Impact of a diagnostic cerebrospinal fluid enterovirus polymerase chain reaction test on patient management. JAMA. 2000;283:2680-5.
7. Nijhuis M, Van Maarseveen N, Schuurman R, Verkuijlen S, De Vos M, Hendriksen K, et al. Rapid and sensitive routine detection of all members of the genus enterovirus in different clinical specimens by real-time PCR. J Clin Microbiol. 2002;40:3666-70.
8. Kost CB, Rogers B, Oberste MS, Robinson C, Eaves BL, Leos K, et al. Multicenter beta trial of the GeneXpert enterovirus assay. J Clin Microbiol. 2007;45:1081-6.
9. Marlowe EM, Novak SM, Dunn JJ, Smith A, Cumpio J, Makalintal E, et al. Performance of the GeneXpert enterovirus assay for detection of enteroviral RNA in cerebrospinal fluid. J Clin Virol. 2008;43:110-3.
10. Seme K, Mocilnik T, Komlos KF, Doplihar A, Persing DH, Poljak M. GeneXpert enterovirus assay: one-year experience in a routine laboratory setting and evaluation on three proficiency panels. J Clin Microbiol. 2008;46:1510-3.
11. Verstrepen WA, Bruynseels P, Mertens AH. Evaluation of a rapid real-time RT-PCR assay for detection of enterovirus RNA in cerebrospinal fluid specimens. J Clin Viral. 2002;25(S1):S39-43.
12. Aertsens M, De Canniere P, Moors H. Modelling of silica diffusion experiments with 32Si in Boom Clay. J Contam Hydrol. 2003;61(1-4):117-29.
13. Rotbart HA. Diagnosis of enteroviral meningitis with the polymerase chain reaction. J Pediatr. 1990;117:85-9.
14. Cary J. Assays for virus infection. In: Cann AJ, editor. A practical approach series, virus culture. New York: Oxford University Press; 1999. p. 83-4.
15. Dierssen U, Rehren F, Henke-Gendo C, Harste G, Heim A. Rapid routine detection of enterovirus RNA in cerebrospinal fluid by one-step real time RT-PCR assay". J Clin Virol. 2008;42:58-64.