Monitoring autophagic flux using ref(2)P, the Drosophila p62 ortholog

Cold Spring Harbor Protocols

Volumn 2014, Issue 9, 2014, Pages 959-966

  Devorkin, Lindsay ^b   Gorski, Sharon M ^a,b  

^a BRITISH COLUMBIA CANCER AGENCY   (Canada)

^b SIMON FRASER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEASOME; PROTEIN P62; CADHERIN; DROSOPHILA PROTEIN; NUCLEAR PROTEIN; REFRACTORY TO SIGMA P PROTEIN, DROSOPHILA; SHOTGUN PROTEIN, DROSOPHILA;

ARTICLE; AUTOPHAGY; CHEMOLUMINESCENCE; FLUORESCENCE MICROSCOPY; IMMUNOFLUORESCENCE; NONHUMAN; ORTHOLOGY; PROTEIN AGGREGATION; PROTEIN DEGRADATION; WESTERN BLOTTING; ANIMAL; CYTOLOGY; DROSOPHILA; FEMALE; MALE; METABOLISM; OVARY; PHYSIOLOGY; STARVATION;

ANIMALS; AUTOPHAGY; CADHERINS; DROSOPHILA; DROSOPHILA PROTEINS; FEMALE; MALE; NUCLEAR PROTEINS; OVARY; STARVATION;

EID: 84923925217     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot080333     Document Type: Article

References (12)

1. Barth JMI, Szabad J, Hafen E, Kohler K. 2011. Autophagy in Drosophila ovaries is induced by starvation and is required for oogenesis. Cell Death Differ 18: 915–924.
2. Bartlett BJ, Isakson P, Lewerenz J, Sanchez H, Kotzebue RW, Cumming RC, Harris GL, Nezis IP, Schubert DR, Simonsen A, et al. 2011. p62, Ref(2)P and ubiquitinated proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects. Autophagy 7: 572–583.
3. Cumming RC, Simonsen A, Finley KD. 2008. Quantitative analysis of autophagic activity in Drosophila neural tissues by measuring the turnover rates of pathway substrates. Meth Enzymol 451: 639–651.
4. DeVorkin L, Gorski SM. 2014a. Genetic manipulation of autophagy in the Drosophila ovary. Cold Spring Harb Protoc doi: 10.1101/pdb.prot080358.
5. DeVorkin L, Gorski SM. 2014b. Monitoring autophagy in Drosophila using fluorescent reporters in the UAS-GAL4 system. Cold Spring Harb Protoc doi: 10.1101/pdb.prot080341.
6. DeVorkin L, Gorski SM. 2014c. LysoTracker staining to aid in monitoring autophagy in Drosophila. Cold Spring Harb Protoc doi: 10.1101/pdb.prot080325.
7. Dezelee S, Bras F, Contamine D, Lopez-Ferber M, Segretain D, Teninges D. 1989. Molecular analysis of ref(2)P, a Drosophila gene implicated in sigma rhabdovirus multiplication and necessary for male fertility. EMBO J 8: 3437–3446.
8. Fujita K-I, Maeda D, Xiao Q, Srinivasula SM. 2011. Nrf2-mediated induction of P62 controls Toll-like receptor-4–driven aggresome-like induced structure formation and autophagic degradation. Proc Natl Acad Sci 108: 1427–1432.
9. Lewis EB. 1960. A new standard food medium. Drosophila Information Service 34: 117–118.
10. Nakaso K, Yoshimoto Y, Nakano T, Takeshima T, Fukuhara Y, Yasui K, Araga S, Yanagawa T, Ishii T, Nakashima K. 2004. Transcriptional activation of p62/A170/ZIP during the formation of the aggregates: Possible mechanisms and the role in Lewy body formation in Parkinson’s disease. Brain Res 1012: 42–51.
11. Nezis IP, Simonsen A, Sagona AP, Finley K, Gaumer S, Contamine D, Rusten TE, Stenmark H, Brech A. 2008. Ref(2)P, the Drosophila melanogaster homologue of mammalian p62, is required for the formation of protein aggregates in adult brain. J Cell Biol 180: 1065–1071.
12. Pircs K, Nagy P, Varga A, Venkei Z, Erdi B, Hegedus K, Juhasz G. 2012. Advantages and limitations of different p62–based assays for estimating autophagic activity in Drosophila. PLoS ONE 7: e44214.