Purification of mitochondria by sucrose step density gradient centrifugation

Cold Spring Harbor Protocols

Volumn 2014, Issue 10, 2014, Pages 1115-1117

  Clayton, David A ^a   Shadel, Gerald S ^b  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; MITOCHONDRION; PURIFICATION; RUPTURE; SUCROSE DENSITY GRADIENT CENTRIFUGATION; TISSUE CULTURE CELL; ANIMAL; CELL CULTURE; CELL FRACTIONATION; DENSITY GRADIENT CENTRIFUGATION; DEVICES; HUMAN; LIVER; ORGAN CULTURE TECHNIQUE; PHYSIOLOGY; PROCEDURES; ULTRASTRUCTURE;

SUCROSE;

ANIMALS; CELL FRACTIONATION; CELLS, CULTURED; CENTRIFUGATION, DENSITY GRADIENT; HUMANS; LIVER; MITOCHONDRIA; ORGAN CULTURE TECHNIQUES; SUCROSE;

EID: 84923888465     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot080028     Document Type: Article

References (5)

1. Clayton DA, Shadel GS. 2014a. Isolation of mitochondria from tissue culture cells. Cold Spring Harb Protoc doi: 10.1101/pdb.prot 080002.
2. Clayton DA, Shadel GS. 2014b. Isolation of mitochondria from animal tissue. Cold Spring Harb Protoc doi: 10.1101/pdb.prot080010.
3. de Duve C. 1971. Tissue fractionation—Past and present. J Cell Biol 50: 20d–55d.
4. de Duve C, Beaufay H. 1981. A short history of tissue fractionation. J Cell Biol 91: 293S–299S.
5. Graham J, Ford T, Rickwood D. 1994. The preparation of subcellular organelles from mouse liver in self-generated gradients of iodixanol. Anal Biochem 220: 367–373.