Single-neuron isolation for RNA analysis using pipette capture and laser capture microdissection

Cold Spring Harbor Protocols

Volumn 2015, Issue 1, 2015, Pages 60-68

  Lovatt, Ditte ^a   Bell, Thomas ^a   Eberwine, James ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCRIPTOME; RNA;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL ISOLATION; COMPUTER PROGRAM; GENE AMPLIFICATION; HIPPOCAMPUS; ISOLATED ORGAN; LASER CAPTURE MICRODISSECTION; MICROMANIPULATOR; MICROSCOPY; MOUSE; NEURON ISOLATION; NONHUMAN; PHOTOGRAPHY; PIPETTE CAPTURE MICRODISSECTION; REVERSE TRANSCRIPTION; RNA ANALYSIS; SOMATIC CELL; TISSUE MICROARRAY; ANIMAL; CELL CULTURE TECHNIQUE; CYTOLOGY; DEVICES; GENETICS; METABOLISM; NERVE CELL; PROCEDURES;

ANIMALS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; HIPPOCAMPUS; LASER CAPTURE MICRODISSECTION; MICE; NEURONS; RNA;

EID: 84923825355     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot072439     Document Type: Article

References (4)

1. Eberwine J, Yeh H, Miyashiro K, Cao Y, Nair S, Finnell R, Zettel M, Coleman P. 1992. Analysis of gene expression in single live neurons. Proc Natl Acad Sci 89: 3010–3014.
2. Mackler S, Eberwine J. 1993. Diversity of glutamate receptor subunit mRNA expression within live hippocampal CA1 neurons. Mol Pharm 44: 308–315.
3. Morris J, Singh JM, Eberwine JH. 2011. Transcriptome analysis of single cells. J Vis Exp.
4. Morris J, Bell TJ, Buckley PT, Eberwine JH. 2014. Antisense RNA amplification for target assessment of total mRNA from a single cell. Cold Spring Harb Protoc doi: 10.1101/pdb.prot072454.