Real-time cellular exometabolome analysis with a microfluidic-mass spectrometry platform

PLoS ONE

Volumn 10, Issue 2, 2015, Pages

  Marasco, Christina C ^a,b   Enders, Jeffrey R ^a,c,d   Seale, Kevin T ^a,b   McLean, John A ^a,c   Wikswo, John P ^a,b,c  

^a VANDERBILT UNIVERSITY   (United States)

^b VANDERBILT UNIVERSITY   (United States)

^c VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d Ameritox Ltd   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BENZOYLECGONINE; COCAINE; DIMETICONE; SODIUM CHLORIDE;

ADSORPTION; ANALYTIC METHOD; ARTICLE; AUTOCRINE EFFECT; AUTOMATION; BIOREACTOR; CELL COMMUNICATION; CELL MANIPULATION; CELL METABOLISM; CELL STIMULATION; CELLULAR SECRETION; CENTRIFUGATION; CHEMICAL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; DILUTION; DRUG EXPOSURE; ELECTROSPRAY MASS SPECTROMETRY; EXOMETABOLOME; HUMAN; HUMAN CELL; IMMUNOLOGICAL MEMORY; INFORMATION PROCESSING; JURKAT CELL LINE; MASS SPECTROMETRY; METABOLOME; METABOLOMICS; MICROENVIRONMENT; MICROFLUIDIC ANALYSIS; NUTRIENT UPTAKE; ONLINE ANALYSIS; PARACRINE SIGNALING; QUALITY CONTROL; SAMPLING; SIGNAL TRANSDUCTION; SOLID PHASE EXTRACTION; SYSTEMS BIOLOGY; T LYMPHOCYTE; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; UPREGULATION; EXTRACELLULAR SPACE; METABOLISM; MICROFLUIDICS; PROCEDURES;

COCAINE; EXTRACELLULAR SPACE; HUMANS; JURKAT CELLS; MASS SPECTROMETRY; METABOLOME; METABOLOMICS; MICROFLUIDICS; SOLID PHASE EXTRACTION; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84923770649     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0117685     Document Type: Article

References (34)

1. Wikswo JP (2014) The relevance and potential roles of microphysiological systems in biology and medicine. Exp Biol Med 239: 1061-1072. doi: 10.1177/1535370214542068 PMID: 25187571
2. Kell DB, Brown M, Davey HM, Dunn WB, Spasic I, et al. (2005) Metabolic footprinting and systems biology: the medium is the message. Nat Rev Microbiol 3: 557-565. PMID: 15953932
3. LeDuc PR, Messner WC, Wikswo JP (2011) How do control-based approaches enter into biology? Annu Rev Biomed Engr 13: 369-396. doi: 10.1146/annurev-bioeng-071910-124651 PMID: 21599491
4. Faley S, Copland M, Reboud J, Cooper JM (2012) Intracellular protein trafficking kinetics in chronic myeloid leukemia stem cells using a microfluidic platform. Integr Biol 4: 368-373. doi: 10.1039/c2ib00086e PMID: 22344285
5. Faley SL, Copland M, Reboud J, Cooper JM (2011) Cell chip array for microfluidic proteomics enabling rapid in situ assessment of intracellular protein phosphorylation. Biomicrofluidics 5: 024106.
6. Wlodkowic D, Faley S, Darzynkiewicz Z, Cooper JM (2011) Real-time cytotoxicity assays. In: Cree IA, editor. Cancer cell culture. Humana Press. pp. 285-291.
7. Faley SL, Copland M, Wlodkowic D, Kolch W, Seale KT, et al. (2009) Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells. Lab Chip 9: 2659-2664. doi: 10.1039/b902083g PMID: 19704981
8. Seale KT, Faley SL, Chamberlain J, Wikswo JP Jr (2010) Macro to nano: A simple method for transporting cultured cells from milliliter scale to nanoliter scale. Exp Biol Med 235: 777-783. doi: 10.1258/ebm.2010.009379 PMID: 20511682
9. Hughey JJ, Wikswo JP, Seale KT (2007) Intra-microfluidic pinocytic loading of Jurkat T cells. In: IEEE/NIH Life Science Systems and Applications Workshop. Piscataway, NY: IEEE. pp. 132-135.
10. Byrd TF, Hoang LT, Kim EG, Pfister ME, Werner EM, et al. (2014) The microfluidic multitrap nanophysiometer for hematologic cancer cell characterization reveals temporal sensitivity of the calcein-AM efflux assay. Sci Rep 4: Article 5117. doi: 10.1038/srep07387 PMID: 25552271
11. Shotwell MS, Drake KJ, Sidorov VY, Wikswo JP (2013) Mechanistic analysis of challenge-response experiments. Biometrics 69: 741-747. doi: 10.1111/biom.12066 PMID: 23859366
12. Yates JR (2000) Mass spectrometry - from genomics to proteomics. Trends Genet 16: 5-8. PMID: 10637622
13. Aebersold R, Mann M (2003) Mass spectrometry-based proteomics. Nature 422: 198-207. PMID: 12634793
14. Han XL, Gross RW (2003) Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics. J Lipid Res 44: 1071-1079. PMID: 12671038
15. Zaia J (2004) Mass spectrometry of oligosaccharides. Mass Spectrom Rev 23: 161-227. PMID: 14966796
16. Wikswo JP, Block FE III, Cliffel DE, Goodwin CR, Marasco CC, et al. (2013) Engineering challenges for instrumenting and controlling integrated organ-on-chip systems. IEEE Trans Biomed Eng 60: 682-690. doi: 10.1109/TBME.2013.2244891 PMID: 23380852
17. Wikswo J, Curtis EL, Eagleton ZE, Evans BC, Kole A, et al. (2013) Scaling and systems biology for integrating multiple organs-on-a-chip. Lab Chip 13: 3496-3511. doi: 10.1039/c3lc50243k PMID: 23828456
18. Chan JH, Timperman AT, Qin D, Aebersold R (1999) Microfabricated polymer devices for automated sample delivery of peptides for analysis by electrospray ionization tandem mass spectrometry. Anal Chem 71: 4437-4444. PMID: 10546527
19. Jiang Y, Wang PC, Locascio LE, Lee CS (2001) Integrated plastic microfluidic devices with ESI-MS for drug screening and residue analysis. Anal Chem 73: 2048-2053. PMID: 11354489
20. Chen Q, Wu J, Zhang Y, Lin JM (2012) Qualitative and quantitative analysis of tumor cell metabolism via stable isotope labeling assisted microfluidic chip electrospray ionization mass spectrometry. Anal Chem 84: 1695-1701. doi: 10.1021/ac300003k PMID: 22242916
21. Enders JR, Marasco CC, Kole A, Nguyen B, Sundarapandian S, et al. (2010) Towards monitoring real-time cellular response using an integrated microfluidics-MALDI/NESI-ion mobility-mass spectrometry platform. IET Syst Biol 4: 416-427. doi: 10.1049/iet-syb.2010.0012 PMID: 21073240
22. Enders JR, Marasco CC, Wikswo JP, Mclean JA (2012) A dual-column solid phase extraction strategy for online collection and preparation of continuously flowing effluent streams for mass spectrometry. Anal Chem 84: 8467-8474. doi: 10.1021/ac3021032 PMID: 22967262
23. Bagasra O, Forman L (1989) Functional analysis of lymphocytes subpopulations in experimental cocaine abuse.1. Dose-dependent activation of lymphocyte subsets. Clin Exp Immunol 77: 289-293. PMID: 2528433
24. Roth MD, Whittaker KM, Choi R, Tashkin DP, Baldwin GC (2005) Cocaine and sigma-1 receptors modulate HIV infection, chemokine receptors, and the HPA axis in the huPBL-SCID model. J Leukoc Biol 78: 1198-1203. PMID: 16204638
25. Chao C, Jacobson LP, Tashkin D, Martinez-Maza O, Roth MD, et al. (2008) Recreational drug use and T lymphocyte subpopulations in HIV-uninfected and HIV-infected men. Drug Alcohol Depen 94: 165-171. doi: 10.1016/j.drugalcdep.2007.11.010 PMID: 18180115
26. Pellegrino T, Bayer BM (1998) In vivo effects of cocaine on immune cell function. J Neuroimmunol 83: 139-147. PMID: 9610682
27. Kubera M, Filip M, Basta-Kaim A, Nowak E, Siwanowicz J, et al. (2004) The effect of cocaine sensitization on mouse immunoreactivity. Eur J Pharmacol 483: 309-315. PMID: 14729122
28. Duffy DC, McDonald JC, Schueller OJA, Whitesides GM (1998) Rapid prototyping of microfluidic systems in poly(dimethylsiloxane). Anal Chem 70: 4974-4984. doi: 10.1021/ac980656z PMID: 21644679
29. Anderson NL, Anderson NG (2002) The human plasma proteome - History, character, and diagnostic prospects. Mol Cell Proteomics 1: 845-867. PMID: 12488461
30. Xia Y, Wang PP, Bartlett MG, Solomon HM, Busch KL (2000) An LC-MS-MS method for the comprehensive analysis of cocaine and cocaine metabolites in meconium. Anal Chem 72: 764-771. PMID: 10701261
31. Fandino AS, Toennes SW, Kauert GF (2002) Studies on hydrolytic and oxidative metabolic pathways of anhydroecgonine methyl ester (methylecgonidine) using microsomal preparations from rat organs. Chem Res Toxicol 15: 1543-1548. PMID: 12482236
32. Darby S, Moore M, Wikswo JP, Reiserer R, Friedlander T, et al. (2010) A metering rotary nanopump for microfluidic systems. Lab Chip 10: 3218-3226. doi: 10.1039/c0lc00087f PMID: 20959938
33. Pacifici R, Zuccaro P, Farre M, Pichini S, Di Carlo S, et al. R (2002) Cell-mediated immune response in MDMA users after repeated dose administration. Ann New York Acad Sci 965: 421-433. PMID: 12105117
34. Warner A, Norman AB (2000) Mechanisms of cocaine hydrolysis and metabolism in vitro and in vivo: A clarification. Ther Drug Monit 22: 266-270. PMID: 10850392