Exploring brain genoarchitecture by single and double chromogenic in situ hybridization (ISH) and immunohistochemistry (IHC) in whole-mount embryos

Neuromethods

Volumn 99, Issue , 2015, Pages 61-82

  Ferran, José Luis ^a   Ayad, Abdelmalik ^a   Merchán, Paloma ^a   Morales Delgado, Nicanor ^a   Sánchez Arrones, Luisa ^a   Alonso, Antonia ^a   Sandoval, Juan Eugenio ^a   Bardet, Sylvia Marianne ^a   Corral San Miguel, Rubén ^a   Sánchez Guardado, Luis Óscar ^a   Hidalgo Sánchez, Matías ^a   Martínez de la Torre, Margaret ^a   Puelles, Luis ^a  

^a NONE

Author keywords

Digoxigenin; FITC; Fluorescein; Gene expression patterns; In situ hybridization; Labeled; Riboprobes; RNA detection; RNA probes; Whole mount

Indexed keywords

CHLOROFORM; COMPLEMENTARY DNA; MESSENGER RNA; PHENOL;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN DEVELOPMENT; BRAIN HISTOLOGY; BRAIN TISSUE; CHICKEN; CLINICAL PROTOCOL; CONTROLLED STUDY; DNA SEQUENCE; DNA TEMPLATE; EMBRYO; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE STRUCTURE; GENOARCHITECTURE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; NEUROANATOMY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA PROBE;

AMPHIBIA; ANIMALIA; AVES; MAMMALIA;

EID: 84923646097     PISSN: 08932336     EISSN: 19406045     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2303-8_4     Document Type: Article

References (11)

1. Ferran JL, Sanchez-Arrones L, Sandoval JE, Puelles L (2007) A model of early molecular regionalization in the chicken embryonic pretectum. J Comp Neurol 505(4):379-403
2. Sanchez-Arrones L, Ferran JL, Rodriguez- Gallardo L, Puelles L (2009) Incipient forebrain boundaries traced by differential gene expression and fate mapping in the chick neural plate. Dev Biol 335(1):43-65
3. Sanchez-Guardado LO, Irimia M, Sanchez- Arrones L, Burguera D, Rodriguez-Gallardo L, Garcia-Fernandez J, Puelles L, Ferran JL, Hidalgo-Sanchez M (2011) Distinct and redundant expression and transcriptional diversity of MEIS gene paralogs during chicken development. Dev Dyn 240(6): 1475-1492
4. Puelles L, Ferran JL (2012) Concept of neuronal genoarchitecture and its genomic fundament. Front Neuroanat 6:47
5. Henrique D et al (1995) Expression of a Delta homologue in prospective neurons in the chick. Nature 375(6534):787-790
6. SternC (2001) http://www.anat.ucl.ac.uk/ research/sternlab/INSITU.htm
7. Theiler K (1989) The house mouse. Atlas of embryonic development, 2nd edn. Springer, New York
8. Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49-92
9. Ainsworth SJ et al (2001) Developmental stages of the Japanese quail. J Anat 216:3-15
10. Chapman SC et al (2001) Improved method for chick whole-embryo culture using a filter paper carrier. Dev Dyn 220(3):284-289
11. Psychoyos D., Finnell R. (2008) Double whole mount in situ hybridization of early chick embryos. JoVE (20). doi: 10.3791/904