DNA typing of ancient parasite eggs from environmental samples identifies human and animal worm infections in viking-age settlement

Journal of Parasitology

Volumn 101, Issue 1, 2015, Pages 57-63

  Søe, Martin Jensen ^a,b   Nejsum, Peter ^a   Fredensborg, Brian Lund ^a   Kapel, Christian Moliin Outzen ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RIBOSOME RNA; RNA 18S;

DNA; EGG; GENETIC MARKER; GEOGRAPHICAL DISTRIBUTION; IDENTIFICATION KEY; INFECTIVITY; MORPHOLOGY; PARASITE;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ARTICLE; ASCARIS; BOVINAE; CONTROLLED STUDY; DENMARK; DNA DETERMINATION; DNA FINGERPRINTING; DNA SEQUENCE; EGG; ENVIRONMENT; FASCIOLA; FECES; GENE EXPRESSION; GENETIC MARKER; HELMINTHIASIS; HOST PARASITE INTERACTION; HUMAN; MORPHOLOGY; NONHUMAN; PARASITE IDENTIFICATION; PARASITISM; SEQUENCE HOMOLOGY; SHEEP; SOIL; SPECIES IDENTIFICATION; SWINE; TRICHURIS; TRICHURIS TRICHIURA; ANIMAL; ASCARIASIS; BOVINE; CATTLE DISEASE; CLASSIFICATION; FASCIOLA HEPATICA; FASCIOLIASIS; GENETICS; HISTORY; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; OOCYTE; PALEOPATHOLOGY; PARASITOLOGY; SHEEP DISEASE; TRICHURIASIS;

DENMARK; VIBORG;

ANIMALIA; ASCARIS SP.; BOS; FASCIOLA HEPATICA; FASCIOLA SP.; OVIS ARIES; SUIDAE; TRICHURIS; TRICHURIS TRICHIURA;

ANIMALS; ASCARIASIS; ASCARIS; CATTLE; CATTLE DISEASES; DENMARK; DNA FINGERPRINTING; FASCIOLA HEPATICA; FASCIOLIASIS; HISTORY, MEDIEVAL; HUMANS; MOLECULAR SEQUENCE DATA; OVUM; PALEOPATHOLOGY; SHEEP; SHEEP DISEASES; TRICHURIASIS; TRICHURIS;

EID: 84923580569     PISSN: 00223395     EISSN: 19372345     Source Type: Journal    
DOI: 10.1645/14-650.1     Document Type: Article

References (29)

1. AMER, S., Y. DAR, M. ICHIKAWA, Y. FUKUDA, C. TADA, T. ITAGAKI, AND Y. NAKAI. 2011. Identification of Fasciola species isolated from Egypt based on sequence analysis of genomic (ITS1 and ITS2) and mitochondrial (NDI and COI) gene markers. Parasitology International 60: 5-12.
2. ANDERSEN, L. O., D. ROSER, P. NEJSUM, H. V. NIELSEN, AND C. R. STENSVOLD. 2013. Is supplementary bead beating for DNA extraction from nematode eggs by use of the NucliSENS easyMAG protocol necessary? Journal of Clinical Microbiology 51: 1345-1347.
3. BETSON, M., P. NEJSUM, R. BENDALL, R. M. DEB, AND J. R. STOTHARD. 2014. Molecular epidemiology of ascariasis: A global perspective on the transmission dynamics of Ascaris in people and pigs. Journal of Infectious Diseases 210: 1-10.
4. BOUCHET, F., D. BAFFIER, M. GIRARD, P. MOREL, J. C. PAICHELER, AND F. DAVID. 1996. Paleoparasitologie en contexte pleistocene: premieres observations a la Grande Grotte d'Arcy-sur-Cure (Yonne), France. Comptes rendus de l'Academie des Sciences 319: 147-151.
5. BOTELLA, H. G., J. A. A. VARGAS, M. A. ROSA, D. LELES, E. G. REIMERS, A. C. P. VICENTE, AND A. M. INIGUEZ. 2010. Paleoparasitologic, paleogenetic and paleobotanic analysis of XVIII Century coprolites from the church La Concepcion in Santa Cruz de Tenerife, Canary Islands, Spain. Memorias do Instituto Oswaldo Cruz 105: 1054-1056.
6. CLEELAND, L. M., M. V. REICHARD, R. Y. TITO, K. J. REINHARD, AND C. M. LEWIS. 2013. Clarifying prehistoric parasitism from a complementary morphological and molecular approach. Journal of Archaeological Sciences 40: 3060-3066.
7. DITTMAR, K., AND W. R. TEEGEN. 2003. The presence of Fasciola hepatica (liver-fluke) in humans and cattle from a 4, 500 year old archaeological site in the Saale-Unstrut Valley, Germany. Memorias do Instituto Oswaldo Cruz 98(Suppl. 1): 141-143.
8. FAULKNER, C. T., AND K. J. REINHARD. 2014. A retrospective examination of paleoparasitology and its establishment in the Journal of Parasitology. Journal of Parasitology 100: 253-259.
9. FERREIRA, A. L. F., A. ARAUJO, U. CONFALONIERI, M. CHAME, AND D. C. GOMES. 1991. Trichuris eggs in animal coprolites dated from 30, 000 years ago. Journal of Parasitology 77: 491-493.
10. GONCALVES, M. L. C., A. ARAUJO, AND L. F. FERREIRA. 2003. Human intestinal parasites in the past: New findings and a review. Memorias do Instituto Oswaldo Cruz 98(Suppl. 1): 103-118.
11. HELBæK, H. 1959. Grauballemandens sidste maltid. KUML Arbog for Jysk Arkeologisk Selskab 1958, p. 83-116.
12. INIGUEZ, A. M., K. J REINHARD, A. ARAUJO, L. F. FERREIRA, AND A. C. P. VICENTE. 2003. Enterobius vermicularis: Ancient DNA from North and South American human coprolites. Memorias do Instituto Oswaldo Cruz. 98(Suppl. 1): 67-69.
13. JONSSON, H., A. GINOLHAC, M. SCHUBERT, P. L. F. JOHNSON, AND L. ORLANDO. 2013. mapDamage2.0: Fast approximate Bayesian estimates of ancient DNA damage parameters. Bioinformatics 29: 1682-1684.
14. LELES, D., A. ARAUJO, L. F. FERREIRA, A. C. P. VICENTE, AND A. M. INIGUEZ. 2008. Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: New scenery of ascariasis in pre-Colombian South America times. Memorias do Instituto Oswaldo Cruz 103: 106-108.
15. -, P. CASCARDO, A. D. S. FREIRE, A. MALDONADO, L. SIANTO, AND A. ARAUJO. 2014. Insights about echinostomiasis by paleomolecular diagnosis. Parasitology International 63: 646-649.
16. -, S. GARDNER, K. REINHARD, A. INIGUEZ, AND A. ARAUJO. 2012. Are Ascaris lumbricoides and Ascaris suum a single species. Parasites & Vectors 5: 1-7.
17. LOREILLE, O., E. ROUMAT, O. VERNEAU, F. BOUCHET, AND C. HANNI. 2001. Ancient DNA from Ascaris: Extraction amplification and sequences from eggs collected in coprolites. International Journal for Parasi-tology 31: 1101-1106.
18. MYSKOVA, E., O. DITRICH, B. SAK, M. KVAC, AND T. CYMBALAK. 2014. Detection of ancient DNA of Encephalitozoon intestinalis (Microspo-ridia) in archaeological material. Journal of Parasitology 100: 356-359.
19. NANSEN, P., AND R. J. JøRGENSEN. 1977. Parasite eggs identified in material from archaeological excavations in Ribe (the Viking age). Nord Veterinærmedicin 29: 263-266.
20. OH, C. S., M. SEO, J. Y. CHAI, S. J. LEE, M. J. KIM, J. B. PARK, AND D. H. SHIN. 2010. Amplification and sequencing of Trichuris trichiura ancient DNA extracted from archaeological sediments. Journal of Archaeological Sciences 37: 1269-1273.
21. -,-, N. J. LIM, S. J. LEE, E.-J. LEE, S. D. LEE, AND D. H. SHIN. 2010. Paleoparasitological report on Ascaris aDNA from an ancient East Asian sample. Memorias do Instituto Oswaldo Cruz 105: 225-228.
22. PENG, W., K. YUAN, M. HU, X. ZHOU, AND R. B. GASSER. 2005. Mutation scanning-coupled analysis of haplotypic variability in mitochondrial DNA regions reveals low gene flow between human and porcine Ascaris in endemic regions of China. Electrophoresis 26: 4317-4326.
23. RAVASI, D. F., M. J. O'RIAIN, F. DAVIDS, AND N. ILLING. 2012. Phylogenetic evidence that two distinct Trichuris genotypes infect both humans and non-human primates. PLoS ONE 7: e44187.
24. ROEPSTORFF, A., AND M. PEARMAN. 2005. Parasitter. Viborg Søndersø 1018-1030-Arkæologi Og Naturvidenskab I et Værkstedsomrade Fra Vikingetid, Jysk Arkæologisk Selskab and Viborg Stiftsmuseum, 205-214.
25. ROKNI, M. B., H. MIRHENDI, A. MIZANI, M. MOHEBALI, M. SHARBATKHORI, E. B. KIA, H. ABDOLI, AND S. IZADI. 2010. Identification and differentiation of Fasciola hepatica and Fasciola gigantica using a simple PCR-restriction enzyme method. Experimental Parasitology 124: 209-213.
26. ROTH, H. 1936. Untersuchungen uber die Haufigkeit des Vorkommens von Eingeweidewurmern, speziell von Trichuris trichiura, bei Patienten der Kopenhagener Universitats-Kinderklinik. Acta Paediatrica 19: 104-122.
27. SHIN, D. H., C. S. OH, H. J. LEE, J. Y. CHAI, S. J. LEE, D. W. HONG, S. D. LEE, AND M. SEO. 2013. Ancient DNA analysis on Clonorchis sinensis eggs remained in samples from medieval Korean mummy. Journal of Archaeological Sciences 40: 211-216.
28. WILLERSLEV, E., AND A. COOPER. 2005. Ancient DNA. Proceedings of the Royal Society B-Biology 272: 3-16.
29. YOSHIKAWA, H., M. YAMADA, Y. MATSUMOTO, AND Y. YOSHIDA. 1989. Variation in egg size of Trichuris trichiura. Parasitology Research 75: 649-654.