HPLC separation and ultrasensitive optical quantification of ceramide species applying 7-(diethylamino)coumarin-3-carbonyl azide derivatisation

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 986-987, Issue , 2015, Pages 123-128

  Blaess, Markus ^a   Claus, Ralf A ^a   Deigner, Hans Peter ^b  

^a JENA UNIVERSITY HOSPITAL   (Germany)

^b FURTWANGEN UNIVERSITY   (Germany)

Author keywords

Cellular ceramide quantification; Fluorescence labelling; Reversed phase HPLC

Indexed keywords

CHLORINE COMPOUNDS; CYANIDES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOBILE SECURITY; SEPARATION;

ALKALINE CATALYSTS; BENZOYL CHLORIDES; CELLULAR CERAMIDE QUANTIFICATION; FLUORESCENCE LABELLING; LIMIT OF DETECTION; NANOMOLAR CONCENTRATION; QUANTITATIVE METHOD; REVERSED PHASE HPLC;

CERAMIDES;

7 (DIETHYLAMINO)COUMARIN 3 CARBONYL AZIDE; 9 ANTHRACENECARBONYL CYANIDE; AZIDE; BENZOYL CHLORIDE; CERAMIDE DERIVATIVE; COUMARIN DERIVATIVE; CYANIDE; FLUORESCENT DYE; ORGANIC SOLVENT; UNCLASSIFIED DRUG; 7-DIETHYLAMINOCOUMARIN-3-CARBOHYDRAZIDE; CERAMIDE; HYDRAZINE DERIVATIVE;

ARTICLE; CHEMICAL LABELING; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DERIVATIZATION; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; LIMIT OF DETECTION; LIMIT OF QUANTITATION; OPTICS; PRIORITY JOURNAL; STORAGE; CELL CULTURE; CHEMISTRY; LEUKOCYTE; PROCEDURES; REPRODUCIBILITY; REVERSED PHASE LIQUID CHROMATOGRAPHY; SPECTROFLUOROMETRY;

CELLS, CULTURED; CERAMIDES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; COUMARINS; HUMANS; HYDRAZINES; LEUKOCYTES; LIMIT OF DETECTION; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, FLUORESCENCE;

EID: 84923537218     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2015.02.014     Document Type: Article

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