Quantitative measurement of Ca2+ and Zn2+ in mammalian cells using genetically encoded fluorescent biosensors

Methods in Molecular Biology

Volumn 1071, Issue , 2014, Pages 29-47

  Park, J Genevieve ^a   Palmer, Amy E ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

Calcium; Fluorescence microscopy; F rster resonance energy transfer; Genetically encoded sensors; Live cell imaging; Quantitative; Ratiometric; Zinc

Indexed keywords

ALAMETHICIN; CALCIUM ION; CYSTEINE; DIGITONIN; EDETIC ACID; EGTAZIC ACID; PYRITHIONE; SAPONIN; ZINC ION; CALCIUM; CHELATING AGENT; ZINC;

ARTICLE; BINDING AFFINITY; BIOLOGY; BIOSENSOR; CALCIUM BINDING; CARBOXY TERMINAL SEQUENCE; CELL NUCLEUS; CELL VACUOLE; CYTOSOL; DISSOCIATION CONSTANT; DNA SEQUENCE; ENDOPLASMIC RETICULUM; FLUORESCENCE RESONANCE ENERGY TRANSFER; GOLGI COMPLEX; IN VITRO STUDY; MAMMAL CELL; METAL BINDING; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; CALIBRATION; CELL SURVIVAL; CHEMICAL STRUCTURE; FLUORESCENCE MICROSCOPY; GENETIC PROCEDURES; HELA CELL LINE; HUMAN; METABOLISM; MOLECULAR IMAGING; PROCEDURES; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

MAMMALIA;

BIOSENSING TECHNIQUES; CALCIUM; CALIBRATION; CELL SURVIVAL; CHELATING AGENTS; HELA CELLS; HUMANS; MICROSCOPY, FLUORESCENCE; MODELS, MOLECULAR; MOLECULAR IMAGING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; ZINC;

EID: 84923212007     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-622-1_3     Document Type: Article

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