A simple and rapid DNA extraction method from whole blood for highly sensitive detection and quantitation of HIV-1 proviral DNA by real-time PCR

Journal of Virological Methods

Volumn 214, Issue , 2015, Pages 37-42

  McFall, Sally M ^a   Wagner, Robin L ^a,b   Jangam, Sujit R ^a   Yamada, Douglas H ^a,c   Hardie, Diana ^d   Kelso, David M ^a,e  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CAPE TOWN   (South Africa)

^e Northwestern Global Health Foundation   (United States)

Author keywords

Early infant diagnosis; HIV; Polymerase chain reaction; Provirus

Indexed keywords

VIRUS DNA;

ARTICLE; BLOOD SAMPLING; BLOOD VOLUME; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DNA EXTRACTION; DNA ISOLATION; FILTRATION ISOLATION OF NUCLEIC ACID; GENE DOSAGE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; INFANT; INTERMETHOD COMPARISON; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MAJOR CLINICAL STUDY; NONHUMAN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOUTH AFRICA; BLOOD; EVALUATION STUDY; GENETICS; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; NEWBORN; PROCEDURES; PROVIRUS; STANDARD; STANDARDS; TIME; VIROLOGY; VIRUS LOAD;

HUMAN IMMUNODEFICIENCY VIRUS 1;

BLOOD; DNA, VIRAL; HIV INFECTIONS; HIV-1; HUMANS; INFANT; INFANT, NEWBORN; PROVIRUSES; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; TIME FACTORS; VIRAL LOAD;

EID: 84923097199     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2015.01.005     Document Type: Article

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