Derivation of GMP-compliant integration-free hiPSCs using modified mRNAs

Methods in Molecular Biology

Volumn 1283, Issue , 2015, Pages 31-42

  Durruthy, Jens Durruthy ^a   Sebastiano, Vittorio ^a  

^a NONE

Author keywords

Cell therapy; Clinical use; Gene therapy; GMP; Modified mRNA; Regenerative medicine; Xeno free hiPSCs

Indexed keywords

MESSENGER RNA;

ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; CRYOPRESERVATION; GOOD MANUFACTURING PRACTICE; HUMAN; HUMAN CELL; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; SKIN FIBROBLAST; TARGET CELL; TISSUE CULTURE; BIOLOGICAL THERAPY; CELL DIFFERENTIATION; CULTURE TECHNIQUE; CYTOLOGY; FIBROBLAST; GENETIC TRANSFECTION; GENETICS; METABOLISM; NUCLEAR REPROGRAMMING; PROCEDURES; STANDARDS;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL- AND TISSUE-BASED THERAPY; CELLULAR REPROGRAMMING; CRYOPRESERVATION; FIBROBLASTS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; RNA, MESSENGER; TRANSFECTION;

EID: 84922971968     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/7651_2014_124     Document Type: Article

References (8)

1. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T et al (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872
2. Hacein-Bey-Abina S, Von Kalle C, Schmidt M, McCormack MP, Wulffraat N et al (2003) LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1. Science 302:415–419
3. Dowey SN, Huang X, Chou BK, Ye Z, Cheng L (2012) Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression. Nat Protoc 7:2013–2021
4. Jia F, Wilson KD, Sun N, Gupta DM, Huang M et al (2010) A nonviral minicircle vector for deriving human iPS cells. Nat Methods 7:197–199
5. Cho HJ, Lee CS, Kwon YW, Paek JS, Lee SH et al (2010) Induction of pluripotent stem cells from adult somatic cells by protein-based repro-gramming without genetic manipulation. Blood 116:386–395
6. Merling RK, Sweeney CL, Choi U, De Ravin SS, Myers TG et al (2013) Transgene-free iPSCs generated from small volume peripheral blood non-mobilized CD34+ cells. Blood 121:e98–e107
7. Yoshida Y, Takahashi K, Okita K, Ichisaka T, Yamanaka S (2009) Hypoxia enhances the generation of induced pluripotent stem cells. Cell Stem Cell 5:237–241
8. Durruthy Durruthy J, Ramathal C, Sukhwani M, Fang F, Cui J et al (2014) Fate of induced pluripotent stem cells following transplantation to murine seminiferous tubules. Hum Mol Genet 23:3071–3084