The unphosphorylated EIIANtr protein represses the synthesis of Alkylresorcinols in Azotobacter vinelandii

PLoS ONE

Volumn 10, Issue 2, 2015, Pages

  Muriel Millán, Luis Felipe ^a   Moreno, Soledad ^a   Romero, Yanet ^a,c   Bedoya Pérez, Leidy Patricia ^a   Castañeda, Miguel ^b   Segura, Daniel ^a   Espín, Guadalupe ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^b BENEMÉRITA UNIVERSIDAD AUTÓNOMA DE PUEBLA   (Mexico)

^c UNIVERSIDAD AUTÓNOMA DE GUERRERO   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

ALKYLRESORCINOL; EIIA NTR PROTEIN; LIPID; NITROGEN REGULATORY PROTEIN; PHOSPHOTRANSFERASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; RESORCINOL; RESORCINOL DERIVATIVE;

ARPR GENE; ARTICLE; AZOTOBACTER VINELANDII; BACTERIAL STRAIN; CONTROLLED STUDY; GENE; GENE ACTIVATION; GENE EXPRESSION; GENETIC TRANSCRIPTION; LIPOGENESIS; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION; CHEMISTRY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MUTATION; PHOSPHORYLATION; TRANSCRIPTION INITIATION;

AZOTOBACTER VINELANDII; NEGIBACTERIA;

AZOTOBACTER VINELANDII; BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MUTATION; PHOSPHORYLATION; PHOSPHOTRANSFERASES; RESORCINOLS; TRANSCRIPTIONAL ACTIVATION;

EID: 84922513517     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0117184     Document Type: Article

References (32)

1. Sadoff HL (1975) Encystment and germination in Azotobacter vinelandii. Microbiol Mol Biol Rev 39: 516-539.
2. Reusch RN, Sadoff HL (1983) D-(-)-poly-beta-hydroxybutyrate in membranes of genetically competent bacteria. J Bacteriol 156: 778-788. PMID: 6415039
3. Lin LP, Sadoff HL (1968) Encystment and Polymer Production by Azotobacter vinelandii in the Presence of B-Hydroxybutyrate. J Bacteriol 95: 2336-2343. PMID: 5669905
4. Segura D, Vite O, Romero Y, Moreno S, Castaneda M, et al. (2009) Isolation and characterization of Azotobacter vinelandii mutants impaired in alkylresorcinol synthesis: alkylresorcinols are not essential for cyst desiccation resistance. J Bacteriol 191:3142-3148. doi: 10.1128/JB.01575-08 PMID: 19270099
5. Funa N, Ozawa H, Hirata A, Horinouchi S (2006) Phenolic lipid synthesis by type III polyketide synthases is essential for cyst formation in Azotobacter vinelandii. Proc. Natl. Acad. Sci. USA 103: 6356-6361. PMID: 16597676
6. Romero Y, Moreno S, Guzmán J, Espín G, Segura D (2013) Sigma factor RpoS controls alkylresorcinol synthesis through ArpR, a LysR-type regulatory protein, during encystment of Azotobacter vinelandii. J Bacteriol 195: 1834-1844. doi: 10.1128/JB.01946-12 PMID: 23378510
7. Cocotl-Yañez M, Sampieri A, Moreno S, Núñez C, Castañeda M, et al. (2011) Roles of RpoS and PsrA in cyst formation and alkylresorcinol synthesis in Azotobacter vinelandii. Microbiology 157: 1685-1693. doi: 10.1099/mic.0.046268-0 PMID: 21454367
8. Pflüger-Grau K, Görke B (2010) Regulatory roles of the bacterial nitrogen-related phosphotransferase system. Trends Microbiol 18: 205-214. doi: 10.1016/j.tim.2010.02.003 PMID: 20202847
9. Higa F, Edelstein PH (2001) Potential virulence role of the Legionella pneumophila ptsP ortholog. Infect Immun 69: 4782-4789. PMID: 11447151
10. Ntr under potassium limitation in Rhizobium leguminosarum 3841. PLOS ONE 8: e64682. doi: 10.1371/journal.pone.0064682 PMID: 23724079
11. Michiels J, Van Soom T, D'hooghe I, Dombrecht B, Benhassine T, et al. (1998) The Rhizobium etli rpoN locus: DNA sequence analysis and phenotypical characterization of rpoN,ptsN, and ptsA mutants. J Bacteriol 180: 1729-1740. PMID: 9537369
12. + transporter TrkA. Proc Natl Acad Sci USA 104: 4124-4129. PMID: 17289841
13. Ntr in Escherichia coli. Mol Microbiol 72: 978-994. doi: 10.1111/j.1365-2958.2009.06704.x PMID: 19400808
14. Ntr modulates the phosphate starvation response through interaction with histidine kinase PhoR in Escherichia coli. Mol Microbiol 86: 96-110. doi: 10.1111/j.1365-2958.2012.08176.x PMID: 22812494
15. Ntr are involved in regulation of the poly-β-Hydroxybutyrate biosynthetic genes in Azotobacter vinelandii. J Mol Microbiol Biotechnol 15: 244-254. PMID: 17878711
16. Bali A, Blanco G, Hill S, Kennedy C (1992) Excretion of ammonium by a nifL mutant of Azotobacter vinelandii fixing nitrogen. Appl Environ Microbiol 58: 1711-1718. PMID: 1622243
17. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. New York: Cold Spring Harbor Laboratory Press.
18. Barry T, Geary S, Hannify S, MacGearailt C, Shalloo M, et al. (1992) Rapid mini-preparations of total RNA from bacteria. Nucl Acids Res 20: 4940. PMID: 1383933
19. Cocotl-Yañez M, Moreno S, Encarnación S, López-Pliego L, Castañeda M, et al. (2014) A small heat-shock protein (Hsp20) regulated by RpoS is essential for cyst desiccation resistance in Azotobacter vinelandii. Microbiology 160: 479-487. doi: 10.1099/mic.0.073353-0 PMID: 24385478
20. Hernandez-Eligio A, Moreno S, Castellanos M, Castañeda M, Nuñez C, et al. (2012) RsmA post-transcriptionally controls PhbR expression and polyhydroxybutyrate biosynthesis in Azotobacter vinelandii. Microbiology 158: 11.
21. Alexeyev MF, Shokolenko IN, Croughan TP (1995) Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector construction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67. PMID: 7628718
22. Kovach ME, Elzer PH, Steven Hill D, Robertson GT, Farris MA, et al. (1995) Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166: 175-176. PMID: 8529885
23. -ΔΔC(T) Method. Methods 25: 402-408. PMID: 11846609
24. Segura D, Cruz T, Espin G (2003) Encystment and alkylresorcinol production by Azotobacter vinelandii strains impaired in poly-β-hydroxybutyrate synthesis. Arch Microbiol 179: 437-443. PMID: 12732928
25. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent J Biol Chem 193: 265-275. PMID: 14907713
26. Wilson KJ, Sessitsch A, Corbo JC, Giller KE, Akkermans ADL, et al. (1995) β-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other Gram-negative bacteria. Microbiology 141: 1691-1705. PMID: 7551037
27. Martínez-Salazar JM, Moreno S, Najera R, Boucher JC, Espín G, et al. (1996) Characterization of the genes coding for the putative sigma factor AlgU and its regulators MucA, MucB, MucC, and MucD in Azotobacter vinelandii and evaluation of their roles in alginate biosynthesis. J Bacteriol 178: 1800-1808. PMID: 8606151
28. Ntr-SsrB interaction is required for Salmonella virulence. Proc Natl Acad Sci USA 107: 20506-20511. doi: 10.1073/pnas.1000759107 PMID: 21059960
29. Ntr-dependent regulation of sigma factor selectivity. Mol Microbiol 78: 1468-1483. doi: 10.1111/j.1365-2958.2010.07419.x PMID: 21143318
30. Peralta-Gil M, Segura D, Guzman J, Servin-Gonzalez L, Espin G (2002) Expression of the Azotobacter vinelandii poly-β-hydroxybutirate biosynthetic phbBAC operon is driven by two overlapping promoters and is dependent on the transcriptional activator PhbR. J Bacteriol 184: 5672-5677. PMID: 12270825
31. Hernandez-Eligio A, Castellanos M, Moreno S, Espin G (2011) Transcriptional activation of the Azotobacter vinelandii polyhydroxybutyrate biosynthetic genes phbBAC by PhbR and RpoS. Microbiology 157:3014-3023. doi: 10.1099/mic.0.051649-0 PMID: 21778206
32. Ntr for derepression of Escherichia coli K-12 ilvBN expression. Mol Microbiol 58: 334-344. PMID: 16164569