In vivo phosphorylation of WRKY transcription factor by MAPK

Methods in Molecular Biology

Volumn 1171, Issue , 2014, Pages 171-181

  Ishihama, Nobuaki ^a   Adachi, Hiroaki ^a   Yoshioka, Miki ^a   Yoshioka, Hirofumi ^a  

^a NONE

Author keywords

Anti phosphopeptide antibody; MAPK substrate; WRKY transcription factor

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; WRKY TRANSCRIPTION FACTOR; ANTIBODY; PHOSPHOPEPTIDE; VEGETABLE PROTEIN;

ANTIBODY SPECIFICITY; ARTICLE; GENETIC TRANSCRIPTION; IMMUNOBLOTTING; IN VIVO STUDY; NONHUMAN; PLANT IMMUNITY; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; AGROBACTERIUM; ENZYMOLOGY; GENETIC TRANSFORMATION; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PHOSPHORYLATION; PLANT LEAF; TOBACCO;

AGROBACTERIUM; ANTIBODIES; IMMUNOBLOTTING; MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHOPEPTIDES; PHOSPHORYLATION; PLANT LEAVES; PLANT PROTEINS; TOBACCO; TRANSCRIPTION FACTORS; TRANSFORMATION, GENETIC;

EID: 84922480620     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0922-3_14     Document Type: Article

References (13)

1. Nakagami H, Pitzschke A, Hirt H (2005) Emerging MAP kinase pathways in plant stress signalling. Trends Plant Sci 7: 339-346.
2. Asai S, Ohta K, Yoshioka H (2008) MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana. Plant Cell 20: 1390-1406.
3. Yang K-Y, Liu Y, Zhang S (2001) Activation of a mitogen-activated protein kinase pathway is involved in disease resistance in tobacco. Proc Natl Acad Sci U S A 98: 741-746.
4. Ishihama N, Yamada R, Yoshioka M, Katou S, Yoshioka H (2011) Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response. Plant Cell 23: 1153-1170.
5. Mao G, Meng X, Liu Y, Zheng Z, Chen Z, Zhang S (2011) Phosphorylation of a WRKY transcription factor by two pathogen-responsive MAPKs drives phytoalexin biosynthesis in Arabidopsis. Plant Cell 23: 1639-1653.
6. Andreasson E, Jenkins T, Brodersen P, Thorgrimsen S, Petersen NH, Zhu S, Qiu JL, Micheelsen P, Rocher A, Petersen M, Newman MA, Bjørn NH, Hirt H, Somssich I, Mattsson O, Mundy J (2005) The MAP kinase substrate MKS1 is a regulator of plant defense responses. EMBO J 24: 2579-2589.
7. Menke FL, Kang HG, Chen Z, Park JM, Kumar D, Klessig DF (2005) Tobacco transcription factor WRKY1 is phosphorylated by the MAP kinase SIPK and mediates HR-like cell death in tobacco. Mol Plant Microbe Interact 18: 1027-1034.
8. Ishihama N, Yoshioka H (2012) Posttranslational regulation of WRKY transcription factors in plant immunity. Curr Opin Plant Biol 15: 431-437.
9. Bombarely A, Rosli HG, Vrebalov J, Moffett P, Mueller LA, Martin GB (2012) A draft genome sequence of Nicotiana benthamiana to enhance molecular plant-microbe biology research. Mol Plant Microbe Interact 25: 1523-1530.
10. Popescu SC, Popescu GV, Bachan S, Zhang Z, Gerstein M, Snyder M, Dinesh-Kumar SP (2009) MAPK target networks in Arabidopsis thaliana revealed using functional protein microarrays. Genes Dev 23: 80-92.
11. Mitsuhara I, Ugaki M, Hirochika H, Ohshima M, Murakami T, Gotoh Y, Katayose Y, Nakamura S, Honkura R, Nishimiya S, Ueno K, Mochizuki A, Tanimoto H, Tsugawa H, Otsuki Y, Ohashi Y (1996) Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. Plant Cell Physiol 37: 49-59.
12. Voinnet O, Rivas S, Mestre P, Baulcombe DC (2003) An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 33: 949-956.
13. Cooper HM, Paterson Y (2008) Production of polyclonal antisera. Curr Protoc Mol Biol Chapter 11, Unit 11. 12.