Two cases of false-positive dengue non-structural protein 1 (NS1) antigen in patients with hematological malignancies and a review of the literature on the use of NS1 for the detection of dengue infection

American Journal of Tropical Medicine and Hygiene

Volumn 92, Issue 2, 2015, Pages 367-369

  Chung, Shimin J ^a   Krishnan, Prabha U ^a   Leo, Yee Sin ^a  

^a TAN TOCK SENG HOSPITAL   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE AMINOTRANSFERASE; ASPARTATE AMINOTRANSFERASE; FERRITIN; NONSTRUCTURAL PROTEIN 1; VIRUS PROTEIN;

ADULT; ALANINE AMINOTRANSFERASE BLOOD LEVEL; ARTICLE; ASPARTATE AMINOTRANSFERASE BLOOD LEVEL; CASE REPORT; CHILL; COMPUTER ASSISTED TOMOGRAPHY; DENGUE; DISSEMINATED INTRAVASCULAR CLOTTING; EARLY DIAGNOSIS; FALSE POSITIVE RESULT; FEMALE; FERRITIN BLOOD LEVEL; FEVER; HEMATOLOGIC MALIGNANCY; HUMAN; LETHARGY; MIDDLE AGED; MYELOPROLIFERATIVE DISORDER; NIGHT SWEAT; PALLOR; PANCYTOPENIA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEST STRIP; THROMBIN TIME; AGED; DENGUE VIRUS; FATALITY; IMMUNOLOGY; LABORATORY DIAGNOSIS; LEUKEMIA, MYELOID, ACUTE; LYMPHOMA; POLYMERASE CHAIN REACTION; THROMBOCYTOPENIA;

AGED; DENGUE; DENGUE VIRUS; FALSE POSITIVE REACTIONS; FATAL OUTCOME; FEMALE; FEVER; HUMANS; LEUKEMIA, MYELOID, ACUTE; LYMPHOMA; MIDDLE AGED; POLYMERASE CHAIN REACTION; THROMBOCYTOPENIA; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84922285636     PISSN: 00029637     EISSN: 14761645     Source Type: Journal    
DOI: 10.4269/ajtmh.14-0247     Document Type: Article

References (20)

1. Simmons CP, Farrar JJ, Nguyen vV, Wills B, 2012. Dengue. N Engl J Med 366:1423-1432.
2. World Health Organization, 2009. Dengue: Guidelines for Treatment, Prevention and Control. Geneva: World Health Organization.
3. Andries AC, Duong V, Ngan C, Ong S, Huy R, Sroin KK, Te V, Y B, Try PL, Buchy P, 2012. Field evaluation and impact on clinical management of a rapid diagnostic kit that detects dengue NS1, IgM and IgG. PLoS Negl Trop Dis 6:e1993.
4. Gan VC, Tan KL, Lye DC, Pok KY, Mok SQ, Chua RC, Leo YS, Ng LC, 2014. Diagnosing dengue at point-of-care: utility of a rapid combined diagnostic kit in Singapore. PLoS ONE 9:e90037.
5. Blacksell SD, Jarman RG, Bailey MS, Tanganuchitcharnchai A, Jenjaroen K, Gibbons RV, Paris DH, Premaratna R, de Silva HJ, Lalloo DG, Day NP, 2011. Evaluation of six commercial point-of-care tests for diagnosis of acute dengue infections: the need for combining NS1 antigen and IgM/IgG antibody detection to achieve acceptable levels of accuracy. Clin Vaccine Immunol 18:2095-2101.
6. Lai YL, Chung YK, Tan HC, Yap HF, Yap G, Ooi EE, Ng LC, 2007. Cost-effective real-time PCR (RT-PCR) to screen for dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus. J Clin Microbiol 45:935-941.
7. Santiago GA, Vergne E, Quiles Y, Cosme J, Vazquez J, Medina JF, Colón C, Margolis H, Muñoz-Jordán JL, 2013. Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus. PLoS Negl Trop Dis 7:e2311.
8. Singapore National Environmental Agency. Campaign Against Dengue. Available at: http://dengue.gov. sg/. Accessed March 4, 2014.
9. Alere, 2014. SD Bioline Dengue Duo NS1 Ag + Ab Combo. Available at: http://www.alere.com/ww/en/product-details/sdbiolinedengue-duo-ns1-ag-ab-combo.html. Accessed March 4, 2014.
10. Phuong HL, Thai KT, Nga TT, Giao PT, Hungle Q, Binh TQ, Nam NV, Groen J, de Vries PJ, 2009. Detection of dengue nonstructural 1 (NS1) protein in Vietnamese patients with fever. Diagn Microbiol Infect Dis 63:372-378.
11. Wang SM, Sekaran SD, 2010. Evaluation of a commercial SD dengue virus NS1 antigen capture enzyme-linked immunosorbent assay kit for early diagnosis of dengue virus infection. J Clin Microbiol 48:2793-2797.
12. Pok KY, Lai YL, Sng J, Ng LC, 2010. Evaluation of nonstructural 1 antigen assays for the diagnosis and surveillance of dengue in Singapore. Vector Borne Zoonotic Dis 10:1009-1016.
13. Colombo TE, Vedovello D, Araki CS, Cogo-Moreira H, dos Santos IN, Reis AF, Costa FR, Cruz LE, Casagrande L, Regatieri LJ, Junior JF, Bronzoni RV, Schmidt DJ, Nogueira ML, 2013. Dengue-4 false negative results by Panbiòdengue early ELISA assay in Brazil. J Clin Virol 58:710-712.
14. Felix AC, Romano CM, Centrone Cde C, Rodrigues CL, Villas-Boas L, Araújo ES, de Matos AM, Carvalho KI, Martelli CM, Kallas EG, Pannuti CS, Levi JE, 2012. Low sensitivity of NS1 protein tests evidenced during a dengue type 2 virus outbreak in Santos, Brazil in 2010. Clin Vaccine Immunol 19:1972-1976.
15. Chuansumrit A, Chaiyaratana W, Pongthanapisith V, Tangnararatchakit K, Lertwongrath S, Yoksan S, 2008. The use of dengue nonstructural protein 1 antigen for the early diagnosis during the febrile stage in patients with dengue infection. Pediatr Infect Dis J 27:43-48.
16. Fry SR, Meyer M, Semple MG, Simmons CP, Sekaran SD, Huang JX, McElnea C, Huang CY, Valks A, Young PR, Cooper MA, 2011. The diagnostic sensitivity of dengue rapid test assays is significantly enhanced by using a combined antigen and antibody testing approach. PLoS Negl Trop Dis 5:e1199.
17. Rueda E, Mendez A, Gonzalez G, 2002. Haemophagocytic syndrome associated with dengue hemorrhagic fever. Biomedica 22:160-166.
18. Ramanathan M, Duraisamy G, 1991. Haemophagocytosis in dengue haemorrhagic fever: a case report. Ann Acad Med Singapore 20:803-804.
19. Muller DA, Young PR, 2013. The flavivirus NS1 protein: molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic marker. Antiviral Res 98:192-208.
20. Osorio L, Ramirez M, Bonelo A, Villar LA, Parra B, 2010. Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection. Virol J 7:361.