Dissociation of embryonic kidney followed by re-aggregation as a method for chimeric analysis

Methods in Molecular Biology

Volumn 886, Issue , 2012, Pages 135-146

  Davies, Jamie A ^a   Unbekandt, Mathieu ^a   Ineson, Jessica ^b   Lusis, Michael ^b   Little, Melissa H ^b  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Chimaera; Metanephros; Mosaic; Organ culture; Self organization; Tissue engineering

Indexed keywords

ANIMAL TISSUE; ARTICLE; BRISBANE METHOD; CELL AGGREGATION; CELL CULTURE; EDINBURGH METHOD; EMBRYO; IMMUNOHISTOCHEMISTRY; KIDNEY PARENCHYMA; MOUSE; MOUSE EMBRYO; MUTATION; NONHUMAN; PRIORITY JOURNAL; REVISED EDINBURGH METHOD; STEM CELL; ANIMAL; CELL STRAIN 3T3; CHIMERA; CYTOLOGY; GROWTH, DEVELOPMENT AND AGING; HISTOLOGY; KIDNEY; METHODOLOGY; MICROSCOPY; ORGAN CULTURE TECHNIQUE; PRENATAL DEVELOPMENT; TISSUE ENGINEERING;

CHIMAERIFORMES;

ANIMALS; CHIMERA; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; IMMUNOHISTOCHEMISTRY; KIDNEY; MICE; MICROSCOPY; NIH 3T3 CELLS; ORGAN CULTURE TECHNIQUES; TISSUE ENGINEERING;

EID: 84922124896     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-851-1_12     Document Type: Article

References (8)

1. Unbekandt M, Davies JA (2010) Dissociation of embryonic kidneys followed by reaggregation allows the formation of renal tissues. Kidney Int 77:407-416
2. Lusis M, Li J, Ineson J et al (2010) Isolation of clonogenic, long-term self renewing embryonic renal stem cells. Stem Cell Res 5:23-39
3. Schmidt-Ott KM (2010) ROCK inhibition facilitates tissue reconstitution from embryonic kidney cell suspensions. Kidney Int 77: 387-389
4. Abraham J, Keller C (2010) Renal stem cell biology starts to take spherical shape. Commentary on: Lusis et al., Isolation of clonogenic, long-term self renewing embryonic renal stem cells. Stem Cell Res 5:1-3
5. Siegel N, Rosner M, Unbekandt M et al (2010) Contribution of human amniotic fl uid stem cells to renal tissue formation depends on mTOR. Hum Mol Genet 19(17):3320-3331
6. Ganeva V, Unbekandt M, Davies JA (2011) An improved kidney dissociation and re aggregation culture system results in nephrons arranged organotypically around a single collecting duct system. Organogenesis 7:83-87
7. Davies JA (2010) The embryonic kidney: isolation, organ culture, immunostaining and RNA interference. Methods Mol Biol 633:57-69
8. Rumballe B, Georgas K, Little MH (2008) High-throughput paraf fi n section in situ hybridization and dual immunohistochemistry on mouse tissues. CSH Protoc Jul 1