Gene targeting of human pluripotent stem cells by homologous recombination

Methods in Molecular Biology

Volumn 1114, Issue , 2014, Pages 37-55

  Howden, Sara E ^a   Thomson, James A ^a  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

Bacterial artificial chromosome; Embryonic stem cells; Gene targeting; Homologous recombination; Induced pluripotent stem cells; Transfection

Indexed keywords

ARTICLE; COPY NUMBER VARIATION; FLUORESCENCE IN SITU HYBRIDIZATION; GENE TARGETING; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCREENING; SOUTHERN BLOTTING; STEM CELL EXPANSION; TISSUE CULTURE; BACTERIAL ARTIFICIAL CHROMOSOME; CELL THERAPY; CLONE; DNA MODIFICATION; GENE LOCUS; GENOME; HUMAN TISSUE;

BACTERIA (MICROORGANISMS);

GENE TARGETING; HOMOLOGOUS RECOMBINATION; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; PLURIPOTENT STEM CELLS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 84922017104     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-761-7_4     Document Type: Article

References (3)

1. Yu J, Vodyanik MA, Smuga-Otto K et al (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318:1917-1920
2. Takahashi K, Tanabe K, Ohnuki M et al (2007) Induction of pluripotent stem cells from adult human fi broblasts by defi ned factors. Cell 131: 861-872
3. Howden SE, Gore A, Li Z et al (2011) Genetic correction and analysis of induced pluripotent stem cells from a patient with gyrate atrophy. Proc Natl Acad Sci USA 108:6537-6542