A comparative LC-MS based profiling approach to analyze lipid composition in tissue culture systems

Methods in Molecular Biology

Volumn 1232, Issue , 2015, Pages 103-113

  Atilla Gokcumen, G Ekin ^a   Eggert, Ulrike S ^a  

^a NONE

Author keywords

LC MS based lipid profiling; Lipids; Untargeted lipid profiling

Indexed keywords

LIPID;

ARTICLE; CELL SUSPENSION; CONTROLLED STUDY; DATA PROCESSING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTERMETHOD COMPARISON; LIPID COMPOSITION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; PRIORITY JOURNAL; TISSUE CULTURE; TISSUE CULTURE CELL; ANIMAL; CHEMISTRY; ISOLATION AND PURIFICATION; LIQUID LIQUID EXTRACTION; PROCEDURES; REPRODUCIBILITY; TANDEM MASS SPECTROMETRY; TISSUE CULTURE TECHNIQUE;

ANIMALS; CHROMATOGRAPHY, LIQUID; LIPIDS; LIQUID-LIQUID EXTRACTION; MASS SPECTROMETRY; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY; TISSUE CULTURE TECHNIQUES;

EID: 84921782733     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1752-5_9     Document Type: Article

References (19)

1. Fahy E, Sud M, Cotter D, Subramaniam S (2007) LIPID MAPS online tools for lipid research. Nucleic Acids Res 35(Web Server issue):W606–W612
2. van Meer G, de Kroon AI (2011) Lipid map of the mammalian cell. J Cell Sci 124(Pt 1): 5–8
3. Atilla-Gokcumen GE, Bedigian AV, Sasse S, Eggert US (2011) Inhibition of glycosphingolipid biosynthesis induces cytokinesis failure. J Am Chem Soc 133(26):10010–10013
4. Echard A (2012) Phosphoinositides and cytokinesis: the "PIP" of the iceberg. Cytoskeleton (Hoboken) 69(11):893–912
5. Canals D et al (2010) Differential effects of ceramide and sphingosine 1-phosphate on ERM phosphorylation: probing sphingolipid signaling at the outer plasma membrane. J Biol Chem 285(42):32476–32485
6. Kunkel GT, Maceyka M, Milstien S, Spiegel S (2013) Targeting the sphingosine-1-phosphate axis in cancer, infl ammation and beyond. Nat Rev Drug Discov 12(9):688–702
7. Atilla-Gokcumen GE, Castoreno AB, Sasse S, Eggert US (2010) Making the cut: the chemical biology of cytokinesis. ACS Chem Biol 5(1): 79–90
8. Atilla-Gokcumen GE et al (2014) Dividing cells regulate their lipid composition and localization. Cell 156(3):428–439
9. Saghatelian A, McKinney MK, Bandell M, Patapoutian A, Cravatt BF (2006) A FAAHregulated class of N-acyl taurines that activates TRP ion channels. Biochemistry 45(30): 9007–9015
10. Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purifi cation of total lipides from animal tissues. J Biol Chem 226(1):497–509
11. Zhu ZJ et al (2013) Liquid chromatography quadrupole time-of-fl ight mass spectrometry characterization of metabolites guided by the METLIN database. Nat Protoc 8(3):451–460
12. Tagore R, Thomas HR, Homan EA, Munawar A, Saghatelian A (2008) A global metabolite profi ling approach to identify protein- metabolite interactions. J Am Chem Soc 130(43): 14111–14113
13. Lv H (2013) Mass spectrometry-based metabolomics towards understanding of gene functions with a diversity of biological contexts. Mass Spectrom Rev 32(2):118–128
14. Gerl MJ et al (2012) Quantitative analysis of the lipidomes of the infl uenza virus envelope and MDCK cell apical membrane. J Cell Biol 196(2):213–221
15. Saghatelian A et al (2004) Assignment of endogenous substrates to enzymes by global metabolite profi ling. Biochemistry 43(45): 14332–14339
16. Smith CA et al (2005) METLIN: a metabolite mass spectral database. Ther Drug Monit 27(6):747–751
17. Tautenhahn R et al (2012) An accelerated workfl ow for untargeted metabolomics using the METLIN database. Nat Biotechnol 30(9): 826–828
18. Patti GJ et al (2013) A view from above: cloud plots to visualize global metabolomic data. Anal Chem 85(2):798–804
19. Tautenhahn R, Patti GJ, Rinehart D, Siuzdak G (2012) XCMS online: a web-based platform to process untargeted metabolomic data. Anal Chem 84(11):5035–5039