In vitro analysis of riboswitch-spinach aptamer fusions as metabolite-sensing fluorescent biosensors

Methods in Enzymology

Volumn 550, Issue , 2015, Pages 147-172

  Kellenberger, Colleen A ^a   Hammond, Ming C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cyclic di GMP; Cyclic dinucleotide; Fluorescence imaging; Fluorescence microscopy; RNA biosensor; Spinach aptamer; Vc2 riboswitch

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; ACETIC ACID; ADENOSINE TRIPHOSPHATE; APTAMER; BASE; BORIC ACID; BROMOPHENOL BLUE; CYTIDINE TRIPHOSPHATE; DEOXYADENOSINE TRIPHOSPHATE; DEOXYCYTIDINE TRIPHOSPHATE; DEOXYGUANOSINE TRIPHOSPHATE; EDETIC ACID; GUANOSINE TRIPHOSPHATE; INORGANIC PYROPHOSPHATASE; LIGAND; MAGNESIUM CHLORIDE; PARATHION; POTASSIUM CHLORIDE; PROTEIN BASED RECEPTOR; RECEPTOR; RNA; RNA POLYMERASE; SODIUM CARBONATE; SODIUM CHLORIDE; SPINACH APTAMER; THYMIDINE TRIPHOSPHATE; TRIS BASE; UNCLASSIFIED DRUG; URIDINE TRIPHOSPHATE; XYLENE; XZYLENE CYANOL; RIBOSWITCH;

ARTICLE; BINDING AFFINITY; BINDING KINETICS; BIOSENSOR; DNA TEMPLATE; EQUIPMENT DESIGN; FLUORESCENCE; GENE EXPRESSION; GENERAL DEVICE; IN VITRO STUDY; METABOLITE; METABOLITE SENSING FLUORESCENT BIOSENSOR; MICROPIPETTOR; MICROPLATE READER; MILLIPORE WATER FILTER; MULTICHANNEL MICROPIPETTOR; PH; PRECIPITATION; PRIORITY JOURNAL; RIBOSWITCH; RNA BASED FLUORESCENT BIOSENSOR; RNA PURIFICATION; RNA SYNTHESIS; RNA TRANSCRIPTION; VORTEX MIXER; FLUORESCENCE MICROSCOPY; GENETIC PROCEDURES; GENETICS; PROCEDURES;

SPINACIA OLERACEA;

BIOSENSING TECHNIQUES; MICROSCOPY, FLUORESCENCE; RIBOSWITCH;

EID: 84921403852     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2014.10.045     Document Type: Chapter

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