Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: Some remarks about wound infection

International Wound Journal

Volumn 12, Issue 1, 2015, Pages 47-52

  Bessa, Lucinda J ^a   Fazii, Paolo ^b   Di Giulio, Mara ^a   Cellini, Luigina ^a  

^a UNIVERSITY OF CHIETI   (Italy)

^b SPIRITO SANTO HOSPITAL   (Italy)

Author keywords

Antibiotic susceptibility; Gram positive and Gram negative bacteria; Resistance pattern; Wound infection

Indexed keywords

AMIKACIN; AMOXICILLIN; AMOXICILLIN PLUS CLAVULANIC ACID; AMPICILLIN; ANTIBIOTIC AGENT; CLINDAMYCIN; COTRIMOXAZOLE; DAPTOMYCIN; ERTAPENEM; ERYTHROMYCIN; FOSFOMYCIN; GENTAMICIN; LEVOFLOXACIN; LINEZOLID; MEROPENEM; MOXIFLOXACIN; OXACILLIN; PENICILLIN G; PRULIFLOXACIN; TEICOPLANIN; TETRACYCLINE; VANCOMYCIN; ANTIINFECTIVE AGENT;

ADULT; AGED; ALCALIGENES FAECALIS; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; ASPERGILLUS NIGER; BACTERIUM ISOLATE; CANDIDA ALBICANS; CANDIDA PARAPSILOSIS; CITROBACTER; CITROBACTER KOSERI; CONTROLLED STUDY; CORYNEBACTERIUM; ENTEROCOCCUS; ESCHERICHIA COLI; FEMALE; HUMAN; KLEBSIELLA OXYTOCA; KOCURIA; MAJOR CLINICAL STUDY; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEUS MIRABILIS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS STUTZERI; SPECIES; STAPHYLOCOCCUS AUREUS; WOUND CARE; WOUND INFECTION; ISOLATION AND PURIFICATION; ITALY; MICROBIAL SENSITIVITY TEST; MICROBIOLOGY; MIDDLE AGED; RETROSPECTIVE STUDY; VERY ELDERLY; YOUNG ADULT;

ADULT; AGED; AGED, 80 AND OVER; ANTI-BACTERIAL AGENTS; CORYNEBACTERIUM; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI; FEMALE; HUMANS; ITALY; MALE; MICROBIAL SENSITIVITY TESTS; MIDDLE AGED; PROTEUS MIRABILIS; PSEUDOMONAS AERUGINOSA; RETROSPECTIVE STUDIES; STAPHYLOCOCCUS AUREUS; WOUND INFECTION; YOUNG ADULT;

EID: 84921365456     PISSN: 17424801     EISSN: 1742481X     Source Type: Journal    
DOI: 10.1111/iwj.12049     Document Type: Article

References (34)

1. Meaume S, Keriheul JC, Fromantin I, Téot L. Workload and prevalence of open wounds in the community: French Vulnus initiative. J Wound Care 2012;21:62-6.
2. Cooper R, Lawrence JC. The isolation and identification of bacteria from wounds. J Wound Care 1996;5:335-40.
3. White RJ, Cooper R, Kingsley A. Wound colonization and infection: the role of topical antimicrobials. Br J Nurs 2001;10:563-78.
4. Edwards R, Harding KG. Bacteria and wound healing. Curr Opin Infect Dis 2004;17:91-6.
5. Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev 2001;14:244-69.
6. European Wound Management Association (EWMA). Position document: identifying criteria for wound infection. London: MEP Ltd, 2005.
7. Collier M. Recognition and management of wound infections. 2004. URL http://www.worldwidewounds.com/2004/january/Collier/Management-of-Wound-infections.html [accessed on 12 October 2012]
8. Beldon P. Recognising wound infection. Nurs Times 2001;97:3-4.
9. Howell-Jones RS, Wilson MJ, Hill KE, Howard AJ, Price PE, Thomas DW. A review of the microbiology, antibiotic usage and resistance in chronic skin wounds. J Antimicrob Chemother 2005;55:143-9.
10. Serralta VW, Harrison-Balestra C, Cazzaniga AL, Davis SC, Mertz PM. Lifestyles of bacteria in wounds: presence of biofilms? Wounds 2001;13:29-34.
11. Davies CE, Hill KE, Newcombe RG, Stephens P, Wilson MJ, Harding KG, Thomas DW. A prospective study of the microbiology of chronic venous leg ulcers to reevaluate the clinical predictive value of tissue biopsies and swabs. Wound Repair Regen 2007;15:17-22.
12. Dowd SE, Wolcott RD, Sun Y, McKeehan T, Smith E, Rhoads D. Polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP). PLoS One 2008;3:e3326.
13. Bowler PG, Davies BJ. The microbiology of infected and noninfected leg ulcers. Int J Dermatol 1999;38:573-8.
14. Brook I, Frazier EH. Aerobic and anaerobic microbiology of chronic venous ulcers. Int J Dermatol 1998;37:426-8.
15. Gjødsbøl K, Christensen JJ, Karlsmark T, Jørgensen B, Klein BM, Krogfelt KA. Multiple bacterial species reside in chronic wounds: a longitudinal study. Int Wound J 2006;3:225-31.
16. Davies CE, Hill KE, Wilson MJ, Stephens P, Hill CM, Harding KG, Thomas DW. Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and nonhealing chronic venous leg ulcers. J Clin Microbiol 2004;42:3549-57.
17. Urbancic-Rovan V, Gubina M. Bacteria in superficial diabetic foot ulcers. Diabet Med 2000;17:814-5.
18. Körber A, Schmid EN, Buer J, Klode J, Schadendorf D, Dissemond J. Bacterial colonization of chronic leg ulcers: current results compared with data 5 years ago in a specialized dermatology department. J Eur Acad Dermatol Venereol 2010;24:1017-25.
19. Halbert AR, Stacey MC, Rohr JB, Jopp-McKay A. The effect of bacterial colonization on venous leg ulcer healing. Australas J Dermatol 1992;33:75-80.
20. Madsen SM, Westh H, Danielsen L, Rosdahl VT. Bacterial colonization and healing of venous leg ulcers. APMIS 1996;104:895-9.
21. Burmølle M, Thomsen TR, Fazli M, Dige I, Christensen L, Homøe P, Tvede M, Nyvad B, Tolker-Nielsen T, Givskov M, Moser C, Kirketerp-Møller K, Johansen HK, Høiby N, Jensen PØ, Sørensen SJ, Bjarnsholt T. Biofilms in chronic infections - a matter of opportunity -monospecies biofilms in multispecies infections. FEMS Immunol Med Microbiol 2010;59:324-36.
22. Dissemond J. Methicillin resistant Staphylococcus aureus (MRSA): diagnostic, clinical relevance and therapy. J Dtsch Dermatol Ges 2009;6:544-51.
23. Schmidtchen A, Holst E, Tapper H, Bjorck L. Elastase-producing Pseudomonas aeruginosa degrade plasma proteins and extracellular products of human skin and fibroblasts, and inhibit fibroblast growth. Microb Pathog 2003;34:47-55.
24. Percival SL, Thomas JG, Williams DW. Biofilms and bacterial imbalances in chronic wounds: anti-Koch. Int Wound J 2010;7:169-75.
25. Kirketerp-Møller K, Jensen PØ, Fazli M, Madsen KG, Pedersen J, Moser C, Tolker-Nielsen T, Høiby N, Givskov M, Bjarnsholt T. Distribution, organization, and ecology of bacteria in chronic wounds. J Clin Microbiol 2008;46:2717-22.
26. Baffoni M, Bessa LJ, Grande R, Di Giulio M, Mongelli M, Ciarelli A, Cellini L. Laser irradiation effect on Staphylococcus aureus and Pseudomonas aeruginosa biofilms isolated from venous leg ulcer. Int Wound J 2012;9:517-24.
27. National Institute for Clinical Excellence. Type 2 diabetes. Prevention and management of foot problems. Clinical guideline 10. London: National Institute for Clinical Excellence, 2004.
28. European Wound Management Association (EWMA). Position document: management of wound infection. London: MEP Ltd, 2006.
29. Demling RH, Waterhouse B. The increasing problem of wound bacterial burden and infection in acute and chronic soft-tissue wounds caused by methicillin-resistant Staphylococcus aureus. J Burns Wounds 2007;7:86-98.
30. Nicoletti G, Schito G, Fadda G, Boros S, Nicolosi D, Marchese A, Spanu T, Pantosti A, Monaco M, Rezza G, Cassone A, Garaci E. Bacterial isolates from severe infections and their antibiotic susceptibility pattern in Italy: a nationwide study in the hospital setting. J Chemother 2006;18:589-602.
31. Ge Y, MacDonald D, Hait H, Lipsky B, Zasloff M, Holroyd K. Microbiological profile of infected diabetic foot ulcers. Diabet Med 2002;19:1032-4.
32. Trengove NJ, Stacey MC, McGechie DF, Mata S. Qualitative bacteriology and leg ulcer healing. J Wound Care 1996;5:277-80.
33. Bjarnsholt T, Kirketerp-Møller K, Jensen PØ, Madsen KG, Phipps R, Krogfelt K, Høiby N, Givskov M. Why chronic wounds will not heal: a novel hypothesis. Wound Repair Regen 2008;16:2-10.
34. Cooper RA, Ameen H, Price P, McCulloch DA, Harding KG. A clinical investigation into the microbiological status of "locally infected" leg ulcers. Int Wound J 2009;6:453-62.